Characterization of newly isolated thermotolerant bacterium Cupriavidus sp. CB15 from composting and its ability to produce polyhydroxyalkanoate from glycerol

This study aimed to isolate a novel thermotolerant bacterium that is capable of synthesizing polyhydroxyalkanoate from glycerol under high temperature conditions. A newly thermotolerant polyhydroxyalkanoate (PHA) producing bacterium, Cupriavidus sp. strain CB15, was isolated from corncob compost. Th...

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Bibliographic Details
Published in:	Microbial cell factories Vol. 22; no. 1; p. 68
Main Authors:	Yootoum, Anuyut, Jantanasakulwong, Kittisak, Rachtanapun, Pornchai, Moukamnerd, Churairat, Chaiyaso, Thanongsak, Pumas, Chayakorn, Tanadchangsaeng, Nuttapol, Watanabe, Masanori, Fukui, Toshiaki, Insomphun, Chayatip
Format:	Journal Article
Language:	English
Published:	England BioMed Central Ltd 12-04-2023 BioMed Central BMC
Subjects:	Ammonium chloride Bacteria Biosynthesis Carbon Carbon sources Chemical properties Composting Cooling Cupriavidus Cupriavidus - genetics Cupriavidus - metabolism Cupriavidus necator - genetics Cupriavidus necator - metabolism Cupriavidus sp Dry cells Genomes Glycerin Glycerol Glycerol - metabolism Gram-negative bacteria Growth conditions Heat tolerance (Biology) High temperature Identification and classification Microbiological research Microbiological synthesis Nitrogen NMR Nuclear magnetic resonance Optimization Physiological aspects Poly-3-hydroxybutyrate Polyhydroxyalkanoate Polyhydroxyalkanoates Polyhydroxyalkanoic acid Polyhydroxybutyrate Polymers Production processes Productivity Response surface methodology Shaking Temperature Thermotolerant bacterium Thailand United States > US Germany Taiwan India Glycerol Cupriavidus sp Polyhydroxyalkanoate Thermotolerant bacterium
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Summary:	This study aimed to isolate a novel thermotolerant bacterium that is capable of synthesizing polyhydroxyalkanoate from glycerol under high temperature conditions. A newly thermotolerant polyhydroxyalkanoate (PHA) producing bacterium, Cupriavidus sp. strain CB15, was isolated from corncob compost. The potential ability to synthesize PHA was confirmed by detection of PHA synthase (phaC) gene in the genome. This strain could produce poly(3-hydroxybutyrate) [P(3HB)] with 0.95 g/L (PHA content 75.3 wt% of dry cell weight 1.24 g/L) using glycerol as a carbon source. The concentration of PHA was enhanced and optimized based on one-factor-at-a-time (OFAT) experiments and response surface methodology (RSM). The optimum conditions for growth and PHA biosynthesis were 10 g/L glycerol, 0.78 g/L NH Cl, shaking speed at 175 rpm, temperature at 45 °C, and cultivation time at 72 h. Under the optimized conditions, PHA production was enhanced to 2.09 g/L (PHA content of 74.4 wt% and dry cell weight of 2.81 g/L), which is 2.12-fold compared with non-optimized conditions. Nuclear magnetic resonance (NMR) analysis confirmed that the extracted PHA was a homopolyester of 3-hydyoxybutyrate. Cupriavidus sp. strain CB15 exhibited potential for cost-effective production of PHA from glycerol.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1475-2859 1475-2859
DOI:	10.1186/s12934-023-02059-5