Molecular surveillance of rotavirus infection in Bangui, Central African Republic, October 2011–September 2013

Molecular surveillance of rotavirus infection in Bangui, Central African Republic, October 2011–September 2013

•Molecular surveillance of rotavirus strains in Bangui, Central African Republic 2011–2013.•47% of children under 5years were positive for rotavirus antigen by EIA testing.•The highest detection rates were observed in children less than 5months old (56%).•Different rotavirus strains were co-circulat...

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Published in:Infection, genetics and evolution Vol. 28; pp. 476 - 479
Main Authors: Banga-Mingo, Virginie, Waku-Kouomou, Diane, Gody, Jean Chrysostome, Esona, Mathew D., Yetimbi, Jean Fandema, Mbary-Daba, Regis, Dahl, Benjamin A., Dimanche, Leon, Koyazegbe, Thomas d’Aquin, Tricou, Vianney, Cavallaro, Kathleen F., Guifara, Gilbert, Bowen, Michael D., Gouandjika-Vasilache, Ionela
Format: Journal Article
Language:English
Published: Netherlands Elsevier B.V 01-12-2014
Subjects:
Central African Republic
Central African Republic - epidemiology
Child, Preschool
Gastroenteritis
Gastroenteritis - epidemiology
Gastroenteritis - history
Gastroenteritis - virology
Genotype
History, 21st Century
Humans
Infant
Infant, Newborn
Pediatrics
Population Surveillance
Rotavirus
Rotavirus - classification
Rotavirus - genetics
Rotavirus Infections - epidemiology
Rotavirus Infections - history
Rotavirus Infections - virology
Surveillance
Central African Republic
Pediatrics
Central African Republic
Rotavirus
Surveillance
Gastroenteritis
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Summary:•Molecular surveillance of rotavirus strains in Bangui, Central African Republic 2011–2013.•47% of children under 5years were positive for rotavirus antigen by EIA testing.•The highest detection rates were observed in children less than 5months old (56%).•Different rotavirus strains were co-circulating, 8 G/P combinations identified. The World Health Organization (WHO) recommends the introduction of rotavirus vaccine in the immunization program of all countries. In the Central African Republic (CAR), sentinel surveillance for rotavirus gastroenteritis was established in 2011 by the Ministry of Health, with the support of the Surveillance en Afrique Centrale Project (SURVAC). The purpose of this study was to assess the burden of rotavirus gastroenteritis and to identify rotavirus strains circulating in CAR before the introduction of rotavirus vaccine planned for this year, 2014. One sentinel site and one laboratory at the national level were designated by the CAR Ministry of Health to participate in this surveillance system. Stool samples were collected from children who met the WHO rotavirus gastroenteritis case definition (WHO, 2006). The samples were first screened for group A rotavirus antigen by enzyme immunoassay (EIA), and genotyping assays performed using a multiplex reverse transcriptase PCR (RT-PCR) technique. Between October 2011 and September 2013, 438 stool samples were collected and analyzed for detection of rotavirus antigen; 206 (47%) were positive. Among the 160 (78%) that could be genotyped, G2P[6] was the predominant strain (47%) followed by G1P[8] (25%) and G2P[4] (13%). Almost half of stool samples obtained from children hospitalized with gastroenteritis were positive for rotavirus. These baseline rotavirus surveillance data will be useful to health authorities considering rotavirus vaccine introduction and for evaluating the efficacy of rotavirus vaccine once it is introduced into the routine immunization system.
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ISSN:1567-1348
1567-7257
DOI:10.1016/j.meegid.2014.08.023