Interactions between Verapamil and Digoxin in Langendorff‐Perfused Rat Hearts: The Role of Inhibition of P‐glycoprotein in the Heart

: P‐glycoprotein (P‐gp) is expressed in tumour cells as well as normal tissues including heart. Modulation of P‐gp transport in vivo may lead to increased drug penetrance to tissues with resulting increases in toxicity. We aimed to investigate the effects of P‐gp on the isolated heart by digoxin in...

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Published in:	Basic & clinical pharmacology & toxicology Vol. 107; no. 5; pp. 847 - 852
Main Authors:	Aylin Arici, Mualla, Kilinc, Emrah, Demir, Omer, Ates, Mehmet, Yesilyurt, Alaattin, Gelal, Ayse
Format:	Journal Article
Language:	English
Published:	Oxford, UK Blackwell Publishing Ltd 01-11-2010 Blackwell
Subjects:	Animals ATP-Binding Cassette, Sub-Family B, Member 1 - antagonists & inhibitors Biological and medical sciences Chromatography, High Pressure Liquid digoxin Digoxin - pharmacokinetics Digoxin - pharmacology Drug Interactions Drugs Female Heart Heart Rate - drug effects Heart Ventricles - drug effects Heart Ventricles - metabolism In Vitro Techniques Medical sciences P-Glycoprotein Perfusion Pharmacology. Drug treatments Pressure Rats Rats, Wistar Toxicity Ventricle Ventricular Function, Left - drug effects Verapamil Verapamil - pharmacokinetics Verapamil - pharmacology Heart Cardiotonic agent Digoxin Rat Interaction Calcium antagonist Rodentia P Glycoprotein Antiarrhythmic agent Vertebrata Mammalia Animal Aralkylamine Verapamil Glycoside Inhibitor Circulatory system Digitalis drug
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Summary:	: P‐glycoprotein (P‐gp) is expressed in tumour cells as well as normal tissues including heart. Modulation of P‐gp transport in vivo may lead to increased drug penetrance to tissues with resulting increases in toxicity. We aimed to investigate the effects of P‐gp on the isolated heart by digoxin infusion in the absence and presence of verapamil. The study was performed in Langendorff isolated perfused rat hearts. After a 20 min. stabilisation period with Tyrode Buffer, digoxin (125 μg/5 mL) was infused for 10 min. in the control group (n = 7). The same dose of digoxin was infused during perfusion with verapamil (1 nm) containing Tyrode Buffer (n = 8) in the study group. Outflow concentration and cardiac parameters of digoxin were measured at frequent intervals for 40 min. AUEC(0–40 min) for left ventricular developed pressure was significantly increased in the presence of verapamil (4260 ± 39.37 mmHg min versus 4607 ± 98.09 mmHg min; 95% CI ‐587.7 to −105.8; p = 0.0083). The significant increases in left ventricular developed pressure were at 20, 25, 30, 35 and 40 min. AUC(0–40 min) value for outflow digoxin concentration‐time curve was significantly lower in the presence of verapamil. Verapamil increased the positive inotropic effect of digoxin, probably through the inhibition of P‐gp, which effluxes digoxin out of cardiac cells.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Feature-1
ISSN:	1742-7835 1742-7843
DOI:	10.1111/j.1742-7843.2010.00574.x