In vitro evaluation of anticancer effects of different probiotic strains on HCT‐116 cell line

In vitro evaluation of anticancer effects of different probiotic strains on HCT‐116 cell line

Aim Since the evolution of man, microbes are associated with humans, playing a vital role in the maintenance of good health. However, an imbalance in the gut microbial ecosystem is associated with several diseases including colorectal cancer (CRC). The supplementation with probiotics has been proven...

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Bibliographic Details
Published in:Journal of applied microbiology Vol. 131; no. 4; pp. 1958 - 1969
Main Authors: Yenuganti, V.R., Yadala, R., Azad, R., Singh, S., Chiluka, V., Ahire, J., Reddanna, P.
Format: Journal Article
Language:English
Published: England Oxford University Press 01-10-2021
Subjects:
Anticancer properties
Apoptosis
Bcl-6 protein
Cancer
CASP9 and BCL6
Cell death
Colorectal cancer
Colorectal carcinoma
Cytotoxicity
Dietary supplements
Gene expression
Lactobacillus
Microorganisms
Probiotics
Toxicity
CASP9 and BCL6
colorectal cancer
cell death
Lactobacillus
probiotics
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Summary:Aim Since the evolution of man, microbes are associated with humans, playing a vital role in the maintenance of good health. However, an imbalance in the gut microbial ecosystem is associated with several diseases including colorectal cancer (CRC). The supplementation with probiotics has been proven to be beneficial in improving CRC. In this study, we have evaluated the anticancer effects of 11 probiotic strains on human colorectal carcinoma cell line (HCT‐116). Methods and Results In this study, HCT‐116 cells were treated with various concentrations (0·5, 5, 10, 20 and 200 million CFU per ml) of probiotic strains. The viability was analysed using a MTT assay and IC50 values were determined. Besides this, we evaluated the expression of multiple genes involved in the apoptosis and stress tolerance by real‐time PCR. Lactobacillus reuteri (UBLRu‐87), Saccharomyces boulardii (Unique‐28), Bacillus clausii (UBBC‐07), Bacillus coagulans (Unique‐IS2), Streptococcus salivarius (UBSS‐01), Lactobacillus fermentum (UBLF‐31), Lactobacillus salivarius (UBLS‐22), Bifidobacterium bifidum (UBBB‐55) and Lactobacillus plantarum (UBLP‐40) exhibited potent cytotoxicity on HCT 116 cells. Furthermore, UBLF‐31 and Unique‐28 induced the expression of CJUN, CFOS and CASP‐9, and downregulated the expression of BCL6. UBLRu‐87 and UBBB‐55 induced the expression of CJUN, CFOS and CASP‐9 but not BCL‐6. UBLP‐40, UBBC‐07, UBLS‐22, and Unique‐IS2 induced the expression of CJUN and CASP‐9 and downregulated the expression of BCL‐6. Conclusion These studies indicate the anticancer effects of selected probiotic strains by inducing apoptosis. Significance and Impact of the Study The probiotic strains with the anticancer effects identified in this study can be proposed as potential candidates in the treatment of CRCs.
Bibliography:Vengala Rao Yenuganti and Rajesh Yadala authors contributed equally.
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ISSN:1364-5072
1365-2672
DOI:10.1111/jam.15060