Search Results - "Yeeles, Joseph T P"

Reconstitution of translesion synthesis reveals a mechanism of eukaryotic DNA replication restart by Guilliam, Thomas A., Yeeles, Joseph T. P.

“…Leading-strand template aberrations cause helicase–polymerase uncoupling and impede replication fork progression, but the details of how uncoupled forks are…”
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A conserved mechanism for regulating replisome disassembly in eukaryotes by Jenkyn-Bedford, Michael, Jones, Morgan L., Baris, Yasemin, Labib, Karim P. M., Cannone, Giuseppe, Yeeles, Joseph T. P., Deegan, Tom D.

“…Replisome disassembly is the final step of eukaryotic DNA replication and is triggered by ubiquitylation of the CDC45–MCM–GINS (CMG) replicative helicase 1 – 3…”
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The Escherichia coli Replisome Is Inherently DNA Damage Tolerant by Yeeles, Joseph T. P., Marians, Kenneth J.

“…The Escherichia coli DNA replication machinery must frequently overcome template lesions under normal growth conditions. Yet, the outcome of a collision…”
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Regulated eukaryotic DNA replication origin firing with purified proteins by Yeeles, Joseph T. P., Deegan, Tom D., Janska, Agnieszka, Early, Anne, Diffley, John F. X.

“…Eukaryotic cells initiate DNA replication from multiple origins, which must be tightly regulated to promote precise genome duplication in every cell cycle. To…”
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Protein–DNA complexes are the primary sources of replication fork pausing in Escherichia coli by Gupta, Milind K., Guy, Colin P., Yeeles, Joseph T. P., Atkinson, John, Bell, Hazel, Lloyd, Robert G., Marians, Kenneth J., McGlynn, Peter

“…Replication fork pausing drives genome instability, because any loss of paused replisome activity creates a requirement for reloading of the replication…”
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An Iron-Sulfur Cluster Is Essential for the Binding of Broken DNA by AddAB-type Helicase-Nucleases by Yeeles, Joseph T.P., Cammack, Richard, Dillingham, Mark S.

“…The bacterial helicase-nuclease complex AddAB converts double-stranded DNA breaks into substrates for RecA-dependent recombinational repair. Here we show that…”
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The processing of double-stranded DNA breaks for recombinational repair by helicase–nuclease complexes by Yeeles, Joseph T.P., Dillingham, Mark S.

“…Double-stranded DNA breaks are prepared for recombinational repair by nucleolytic digestion to form single-stranded DNA overhangs that are substrates for…”
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How the Eukaryotic Replisome Achieves Rapid and Efficient DNA Replication by Yeeles, Joseph T.P., Janska, Agnieska, Early, Anne, Diffley, John F.X.

“…The eukaryotic replisome is a molecular machine that coordinates the Cdc45-MCM-GINS (CMG) replicative DNA helicase with DNA polymerases α, δ, and ε and other…”
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Structural basis for processive daughter-strand synthesis and proofreading by the human leading-strand DNA polymerase Pol ε by Roske, Johann J, Yeeles, Joseph T P

“…During chromosome replication, the nascent leading strand is synthesized by DNA polymerase epsilon (Pol ε), which associates with the sliding clamp…”
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Mechanism of Bidirectional Leading-Strand Synthesis Establishment at Eukaryotic DNA Replication Origins by Aria, Valentina, Yeeles, Joseph T.P.

“…DNA replication commences at eukaryotic replication origins following assembly and activation of bidirectional CMG helicases. Once activated, CMG unwinds the…”
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The AddAB helicase-nuclease catalyses rapid and processive DNA unwinding using a single Superfamily 1A motor domain by Yeeles, Joseph T P, Gwynn, Emma J, Webb, Martin R, Dillingham, Mark S

“…The oligomeric state of Superfamily I DNA helicases is the subject of considerable and ongoing debate. While models based on crystal structures imply that a…”
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Cryo-EM Structure of the Fork Protection Complex Bound to CMG at a Replication Fork by Baretić, Domagoj, Jenkyn-Bedford, Michael, Aria, Valentina, Cannone, Giuseppe, Skehel, Mark, Yeeles, Joseph T.P.

“…The eukaryotic replisome, organized around the Cdc45-MCM-GINS (CMG) helicase, orchestrates chromosome replication. Multiple factors associate directly with…”
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The Initial Response of a Eukaryotic Replisome to DNA Damage by Taylor, Martin R.G., Yeeles, Joseph T.P.

“…The replisome must overcome DNA damage to ensure complete chromosome replication. Here, we describe the earliest events in this process by reconstituting…”
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Fast and efficient DNA replication with purified human proteins by Baris, Yasemin, Taylor, Martin R. G., Aria, Valentina, Yeeles, Joseph T. P.

“…Chromosome replication is performed by a complex and intricate ensemble of proteins termed the replisome, where the DNA polymerases Polδ and Polε, DNA…”
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Structure of a human replisome shows the organisation and interactions of a DNA replication machine by Jones, Morgan L, Baris, Yasemin, Taylor, Martin R G, Yeeles, Joseph T P

“…The human replisome is an elaborate arrangement of molecular machines responsible for accurate chromosome replication. At its heart is the CDC45‐MCM‐GINS (CMG)…”
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How Pol α-primase is targeted to replisomes to prime eukaryotic DNA replication by Jones, Morgan L, Aria, Valentina, Baris, Yasemin, Yeeles, Joseph T P

“…During eukaryotic DNA replication, Pol α-primase generates primers at replication origins to start leading-strand synthesis and every few hundred nucleotides…”
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CST–polymerase α-primase solves a second telomere end-replication problem by Takai, Hiroyuki, Aria, Valentina, Borges, Pamela, Yeeles, Joseph T. P., de Lange, Titia

“…Telomerase adds G-rich telomeric repeats to the 3′ ends of telomeres 1 , counteracting telomere shortening caused by loss of telomeric 3′ overhangs during…”
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Mechanisms controlling replication fork stalling and collapse at topoisomerase 1 cleavage complexes by Westhorpe, Rose, Roske, Johann J, Yeeles, Joseph T P

“…Topoisomerase 1 cleavage complexes (Top1-ccs) comprise a DNA-protein crosslink and a single-stranded DNA break that can significantly impact the DNA…”
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An updated perspective on the polymerase division of labor during eukaryotic DNA replication by Guilliam, Thomas A, Yeeles, Joseph T P

“…In eukaryotes three DNA polymerases (Pols), α, δ, and ε, are tasked with bulk DNA synthesis of nascent strands during genome duplication. Most evidence…”
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Visualizing helicases unwinding DNA at the single molecule level by Fili, Natali, Mashanov, Gregory I, Toseland, Christopher P, Batters, Christopher, Wallace, Mark I, Yeeles, Joseph T P, Dillingham, Mark S, Webb, Martin R, Molloy, Justin E

“…DNA helicases are motor proteins that catalyze the unwinding of double-stranded DNA into single-stranded DNA using the free energy from ATP hydrolysis. Single…”
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