Responses of Frog Skin,Rana pipiens, Calcium Ion Transport to Parathyroid Hormone, Calcitonin, and Vitamin D3

Responses of Frog Skin,Rana pipiens, Calcium Ion Transport to Parathyroid Hormone, Calcitonin, and Vitamin D3

The responses of cutaneous Ca2+transport to parathyroid hormone, calcitonin, and vitamin D3were evaluated in intactRana pipiensand isolated skins from this species. Parathyroid hormone (12 pmol g−1) stimulated Ca2+influx in intact frogs but not in isolated skin (10–100 pmol ml−1) unless the frogs ha...

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Bibliographic Details
Published in:General and comparative endocrinology Vol. 112; no. 2; pp. 191 - 199
Main Authors: Stiffler, Daniel F., Yee, J.Christopher, Tefft, J.Denise
Format: Journal Article
Language:English
Published: United States Elsevier Inc 01-11-1998
Subjects:
Animals
Biological Transport - drug effects
Calcitonin - administration & dosage
Calcitonin - pharmacology
Calcium - metabolism
Cholecalciferol - administration & dosage
Cholecalciferol - pharmacology
Dose-Response Relationship, Drug
Drug Synergism
In Vitro Techniques
Parathyroid Hormone - administration & dosage
Parathyroid Hormone - pharmacology
permissive effect of vitamin D3
PTH
Rana pipiens
Skin - drug effects
Skin - metabolism
CT
PTH
permissive effect of vitamin D3
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Summary:The responses of cutaneous Ca2+transport to parathyroid hormone, calcitonin, and vitamin D3were evaluated in intactRana pipiensand isolated skins from this species. Parathyroid hormone (12 pmol g−1) stimulated Ca2+influx in intact frogs but not in isolated skin (10–100 pmol ml−1) unless the frogs had been pretreated with vitamin D3injections (100–300 μg day−1) for 3 days prior to isolating the skins for flux measurements. Pretreatment of intact frogs with vitamin D3for 3 days (100 μg day−1) also resulted in greater responses to PTH (6–12 pmol g−1). Vitamin D3(most likely after conversion to 1,25(OH)2D3) had a direct stimulatory effect on Ca2+transport in isolated skins after three daily doses of 500 μg of D3. Calcitonin also stimulated Ca2+influx in both intact frogs (24 pmol g−1) and isolated skins (25–100 pmol ml−1). We conclude that parathyroid hormone, facilitated by vitamin D, stimulates Ca2+influx in the skin ofR. pipiens. We also conclude that calcitonin is stimulatory for cutaneous Ca2+influx in this species.
ISSN:0016-6480
1095-6840
DOI:10.1006/gcen.1998.7161