Effects of glucose and trehalose on tris-citric acid-egg yolk-fructose diluents for semen cryopreservation in goat

This study aimed to examine the impacts of the wide range of concentrations of glucose and trehalose on the tris-citric acid-egg yolk-fructose (TCEF) extenders for cryopreservation of goat semen. The sperm sample was pooled, washed, and diluted in control (TCEF without glucose and trehalose), TCEF +...

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Published in:	Journal of advanced veterinary and animal research Vol. 10; no. 2; pp. 169 - 177
Main Authors:	Kamal, Md Mostofa, Alam, Md Emtiaj, Das, Sunny Kumar, Yeasmin, Most Shorifa, Ahmed, Soshe, Rahman, Mst Afroza, Das, Dipak Kumar, Gofur, Md Royhan, Masum, Md Abdul
Format:	Journal Article
Language:	English
Published:	Bangladesh Network for the Veterinarians of Bangladesh Bangladesh Agricultural Universityת Faculty of Veterinary Science 01-06-2023 A periodical of the Network for the Veterinarians of Bangladesh (BDvetNET) Network for the Veterinarians of Bangladesh
Subjects:	Acids Balancing buck; cryopreservation; glucose; sperm; sperm parameter; trehalose Citric acid Cryopreservation Diluents Freezing Fructose Glucose Glycerol Goats Integrity Motility Original Phase transitions Plasma Semen Sperm Thawing Trehalose Viability Yolk glucose sperm trehalose Buck cryopreservation sperm parameter
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Summary:	This study aimed to examine the impacts of the wide range of concentrations of glucose and trehalose on the tris-citric acid-egg yolk-fructose (TCEF) extenders for cryopreservation of goat semen. The sperm sample was pooled, washed, and diluted in control (TCEF without glucose and trehalose), TCEF + glucose (75, 150, 450, and 900 mm), and TCEF + trehalose (75, 150, 450, and 900 mm). After equilibrations, the semen straws were frozen under LN2 in the LN2 tank. After LN2 storage, the straws were thawed at 37°C for 30 seconds. The sperm parameters of all study groups were checked after equilibration and freezing. After equilibration, the progressive motility (PM), total motility (TM), and viability of sperm in G-75, G-150, G-450, T-75, T-150, and T-450 were not significantly different ( < 0.05) from those in control. After cryopreservation and thawing, the PM, TM, and plasma membrane integrity (PMI) of T-150 were significantly higher ( < 0.05) than in control, G-75, G-900, T-75, and T-900. The viability of sperm in T-150 was substantially higher ( < 0.05) than in the control, whereas there was no significant difference among the control, G-75, G-900, T-75, and T-900. However, the acrosome integrity (AI) of sperm in G-900 was significantly decreased ( < 0.05) compared to the control, G-75, G-150, G-450, T-75, T-150, and T-450. According to the findings, the supplementation of 150 mm trehalose in the TCEF diluent was more efficient for sperm cryopreservation in the buck as reflected by PM, TM, viability, PMI, and AI.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	2311-7710 2311-7710
DOI:	10.5455/javar.2023.j666