Relevance of Flow Cytometric Auto-Crossmatch to the Post-transplant Course of Kidney Transplant Recipients

Abstract Introduction The crossmatch test is essential prior to kidney transplantation (tx) to confirm compatibility between the donor and the recipient. However, its results can be misleading due to “undetectable antibodies” in the recipient's serum. To establish if undetectable autoantibodies...

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Bibliographic Details
Published in:	Transplantation proceedings Vol. 49; no. 3; pp. 477 - 480
Main Authors:	Demir, E, Yeğit, O, Erol, A, Akgül, S.U, Çalışkan, B, Bayraktar, A, Çalışkan, Y, Türkmen, A, Savran, F.O, Sever, M.S
Format:	Journal Article
Language:	English
Published:	United States Elsevier Inc 01-04-2017
Subjects:	Adult Female Flow Cytometry - methods Graft Rejection - epidemiology Graft Rejection - immunology Graft Survival - immunology Histocompatibility Testing - methods Humans Incidence Kidney Transplantation - methods Male Surgery
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Summary:	Abstract Introduction The crossmatch test is essential prior to kidney transplantation (tx) to confirm compatibility between the donor and the recipient. However, its results can be misleading due to “undetectable antibodies” in the recipient's serum. To establish if undetectable autoantibodies are responsible for a positive result, an auto-crossmatch test can be performed. In this study, we aim to determine the long-term prognostic value of auto-flow cytometric auto-crossmatch (FCXM) test on kidney survival in kidney tx recipients. Materials and Methods The primary outcome variable was reduced renal function. Secondary endpoints were incidence of biopsy-confirmed chronic antibody-mediated rejection (CAMR) and recurrent glomerulonephritis (GN). Results There were no differences regarding initial serum creatinine levels between the study and control groups ( P = .441). Patients who had positive auto-B FCXM had a significantly reduced renal function compared with the control group ( P = .016). Four patients developed biopsy-confirmed CAMR in the study group and 1 patient in the control group ( P = .047). Five patients had biopsy-confirmed recurrent GN in the GN study group, and only 1 patient had recurrent GN in the GN control group ( P = .026). Discussion Kidney transplant recipients with positive auto-FCXM test had significantly reduced renal function and a higher incidence of recurrent GN and CAMR compared with the control group. The findings of this study suggest a potential role of auto-antibody causing positive auto-FCXM test result, meanwhile increasing the risk of CAMR, recurrent GN, and new-onset diabetes after tx.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0041-1345 1873-2623
DOI:	10.1016/j.transproceed.2017.01.023