Time Series Analysis of the Microbiota of Children Suffering From Acute Infectious Diarrhea and Their Recovery After Treatment

Gut microbiota is closely related to acute infectious diarrhea, one of the leading causes of mortality and morbidity in children worldwide. Understanding the dynamics of the recovery from this disease is of clinical interest. This work aims to correlate the dynamics of gut microbiota with the evolut...

Full description

Saved in:

Bibliographic Details
Published in:	Frontiers in microbiology Vol. 9; p. 1230
Main Authors:	Dinleyici, Ener C, Martínez-Martínez, Daniel, Kara, Ates, Karbuz, Adem, Dalgic, Nazan, Metin, Ozge, Yazar, Ahmet S, Guven, Sirin, Kurugol, Zafer, Turel, Ozden, Kucukkoc, Mehmet, Yasa, Olcay, Eren, Makbule, Ozen, Metehan, Martí, Jose Manuel, P Garay, Carlos, Vandenplas, Yvan, Moya, Andrés
Format:	Journal Article
Language:	English
Published:	Switzerland Frontiers Media S.A 12-06-2018
Subjects:	acute infectious diarrhea Microbiology microbiota rotavirus systems biology temporal analysis microbiota systems biology acute infectious diarrhea temporal analysis rotavirus
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Gut microbiota is closely related to acute infectious diarrhea, one of the leading causes of mortality and morbidity in children worldwide. Understanding the dynamics of the recovery from this disease is of clinical interest. This work aims to correlate the dynamics of gut microbiota with the evolution of children who were suffering from acute infectious diarrhea caused by a rotavirus, and their recovery after the administration of a probiotic, CNCM I-745. The experiment involved 10 children with acute infectious diarrhea caused by a rotavirus, and six healthy children, all aged between 3 and 4 years. The children who suffered the rotavirus infection received CNCM I-745 twice daily for the first 5 days of the experiment. Fecal samples were collected from each participant at 0, 3, 5, 10, and 30 days after probiotic administration. Microbial composition was characterized by 16S rRNA gene sequencing. Alpha and beta diversity were calculated, along with dynamical analysis based on Taylor's law to assess the temporal stability of the microbiota. All children infected with the rotavirus stopped having diarrhea at day 3 after the intervention. We observed low alpha diversities in the first 5 days ( -value < 0.05, Wilcoxon test), larger at 10 and 30 days after probiotic treatment. Canonical correspondence analysis (CCA) showed differences in the gut microbiota of healthy children and of those who suffered from acute diarrhea in the first days ( -value < 0.05, ADONIS test), but not in the last days of the experiment. Temporal variability was larger in children infected with the rotavirus than in healthy ones. In particular, class was found to be abundant in children with acute diarrhea. We identified the microbiota transition from a diseased state to a healthy one with time, whose characterization may lead to relevant clinical data. This work highlights the importance of using time series for the study of dysbiosis related to diarrhea.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Edited by: Andrea Masotti, Bambino Gesù Ospedale Pediatrico (IRCCS), Italy This article was submitted to Systems Microbiology, a section of the journal Frontiers in Microbiology These authors have contributed equally to this work. Reviewed by: Francisco José Pérez-Cano, Universitat de Barcelona, Spain; Antonella Celluzzi, Bambino Gesù Ospedale Pediatrico (IRCCS), Italy
ISSN:	1664-302X 1664-302X
DOI:	10.3389/fmicb.2018.01230