Evolving role of Antennapedia protein in arthropod limb patterning

Evolutional changes in homeotic gene functions have contributed to segmental diversification of arthropodan limbs, but crucial molecular changes have not been identified to date. The first leg of the crustacean Daphnia lacks a prominent ventral branch found in the second to fourth legs. We show here...

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Bibliographic Details
Published in:	Development (Cambridge) Vol. 129; no. 15; pp. 3555 - 3561
Main Authors:	Shiga, Yasuhiro, Yasumoto, Ryusuke, Yamagata, Hideo, Hayashi, Shigeo
Format:	Journal Article
Language:	English
Published:	England The Company of Biologists Limited 01-08-2002
Subjects:	Amino Acid Sequence Animals Antennapedia Homeodomain Protein Antennapedia protein Biological Evolution Body Patterning Crustacea Daphnia Daphnia - anatomy & histology Daphnia - embryology Daphnia - genetics Distal-less gene Drosophila melanogaster Drosophila melanogaster - embryology Drosophila melanogaster - genetics Drosophila Proteins Extremities - embryology Genes, Homeobox Homeodomain Proteins - genetics Homeodomain Proteins - metabolism Immunohistochemistry Molecular Sequence Data Nuclear Proteins - metabolism Protein Structure, Tertiary RNA Processing, Post-Transcriptional Sequence Alignment Transcription Factors - metabolism
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Summary:	Evolutional changes in homeotic gene functions have contributed to segmental diversification of arthropodan limbs, but crucial molecular changes have not been identified to date. The first leg of the crustacean Daphnia lacks a prominent ventral branch found in the second to fourth legs. We show here that this phenotype correlates with the loss of Distal-less and concomitant expression of Antennapedia in the limb primordium. Unlike its Drosophila counterpart, Daphnia Antennapedia represses Distal-less in Drosophila assays, and the protein region conferring this activity was mapped to the N terminal region of the protein. The results imply that Dapnia Antennapedia specifies leg morphology by repressing Distal-less , and this activity was acquired through a change in protein structure after separation of crustaceans and insects.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Feature-1
ISSN:	0950-1991 1477-9129
DOI:	10.1242/dev.129.15.3555