Fructose improves titanium dioxide nanoparticles induced alterations in developmental competence of mouse oocytes

We investigated the effects of intraperitoneal injections of titanium dioxide nanoparticles (TiO NPs, 100 mg/kg) for 5 consecutive days on the developmental competence of murine oocytes. Furthermore, study the effects of TiO NPs on antioxidant and oxidative stress biomarkers, as well as their effect...

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Published in:	BMC veterinary research Vol. 20; no. 1; p. 135
Main Authors:	Elmetwally, Mohammed A, Helmy, Amal, Balboula, Ahmed, Eladl, Mohamed, Hamed, Basma, Lashen, Samah, Rezk, Shaymaa, Yaseen, Amira, Sharawy, Heba, Hussien, Mamdouh, Zabel, Samy, Montaser, Abdelmonem, Halawa, Amal
Format:	Journal Article
Language:	English
Published:	England BioMed Central Ltd 03-04-2024 BioMed Central BMC
Subjects:	Antioxidant Antioxidants Apoptosis Biomarkers Body weight Caspase-3 Cell surface Chemical properties Embryos Females Fertility Fructose Growth Hypoxia In vitro fertilization Influence Murine oocytes Nanoparticles Oocytes Ovaries Oxidative stress p53 Protein Physiological aspects Statistical data Structure Testes TiO2 NPs Titanium Titanium dioxide Toxicity Transmission electron microscopy Tumor proteins Egypt Oxidative stress TiO2 NPs Antioxidant Murine oocytes Fructose Apoptosis
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Summary:	We investigated the effects of intraperitoneal injections of titanium dioxide nanoparticles (TiO NPs, 100 mg/kg) for 5 consecutive days on the developmental competence of murine oocytes. Furthermore, study the effects of TiO NPs on antioxidant and oxidative stress biomarkers, as well as their effects on expression of apoptotic and hypoxia inducing factor-1α (HIF1A) protein translation. Moreover, the possible ameliorating effects of intraperitoneal injections of fructose (2.75 mM/ml) was examined. Thirty sexually mature (8-12 weeks old; ~ 25 g body weight) female mice were used for the current study. The female mice were assigned randomly to three treatment groups: Group1 (G1) mice were injected intraperitoneal (ip) with deionized water for 5 consecutive days; Group 2 (G2) mice were injected ip with TiO NPs (100 mg/kg BW) for 5 consecutive days; Group 3 (G3) mice were injected ip with TiO NPs (100 mg/kg BW + fructose (2.75 mM) for 5 consecutive days. Nano-titanium significantly decreased expression of GSH, GPx, and NO, expression of MDA and TAC increased. The rates of MI, MII, GVBD and degenerated oocytes were significantly less for nano-titanium treated mice, but the rate of activated oocytes was significantly greater than those in control oocytes. TiO NPs significantly increased expression of apoptotic genes (BAX, Caspase 3 and P53) and HIF1A. Intraperitoneal injection of fructose (2.75 mM/kg) significantly alleviated the detrimental effects of TiO NPs. Transmission electron microscopy indicated that fructose mitigated adverse effects of TiO2 NPs to alter the cell surface of murine oocytes. Results of this study suggest that the i/p infusion of fructose for consecutive 5 days enhances development of murine oocytes and decreases toxic effects of TiO NPs through positive effects on oxidative and antioxidant biomarkers in cumulus-oocyte complexes and effects to inhibit TiO2-induced increases in expression of apoptotic and hypoxia inducing factors.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1746-6148 1746-6148
DOI:	10.1186/s12917-024-03963-7