Sucrose transporter plays a role in phloem loading in CMV-infected melon plants that are defined as symplastic loaders

Based on the high density of plasmodesmata interconnecting the intermediary cells and their neighboring phloem parenchyma or bundle-sheath cells, and based on the insensitivity to the sucrose transport inhibitor p-chloromercuribenzenesulfonic acid (PCMBS), cucurbits have been concluded to be symplas...

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Bibliographic Details
Published in:	The Plant journal : for cell and molecular biology Vol. 66; no. 2; pp. 366 - 374
Main Authors:	Gil, Lidor, Yaron, Imry, Shalitin, Dror, Sauer, Norbert, Turgeon, Robert, Wolf, Shmuel
Format:	Journal Article
Language:	English
Published:	Oxford, UK Blackwell Publishing Ltd 01-04-2011 Blackwell
Subjects:	bakers yeast Biological and medical sciences Cucumber mosaic virus Cucumis melo Cucumis melo - genetics Cucumis melo - metabolism Cucumis melo - virology Cucumovirus - pathogenicity cucurbit Fruits Fundamental and applied biological sciences. Psychology genes in vitro studies Membrane Transport Proteins - metabolism parenchyma petioles phloem Phloem - metabolism phloem loading Phylogeny Plant biology Plant diseases Plant Leaves - genetics Plant Leaves - metabolism Plant Leaves - virology Plant physiology and development Plant Proteins - metabolism sap Sucrose Sucrose - metabolism sucrose transporter vascular bundles Vegetals Sucrose Biological transport Cucurbitaceae phloem loading sucrose transporter Virus Plant Phloem Loading Dicotyledones Cucumber mosaic virus Angiospermae Bromoviridae Cucumovirus Spermatophyta Cucumis melo cucurbit
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Summary:	Based on the high density of plasmodesmata interconnecting the intermediary cells and their neighboring phloem parenchyma or bundle-sheath cells, and based on the insensitivity to the sucrose transport inhibitor p-chloromercuribenzenesulfonic acid (PCMBS), cucurbits have been concluded to be symplastic loaders. In the present study, we identified and characterized the full-length sequence of sucrose transporter gene (CmSUT1) from melon (Cucumis melo L. cv. Hale's best jumbo). In vitro experiments confirmed that the identified gene product has sucrose transporter activity in baker's yeast. Healthy and cucumber mosaic virus (CMV)-infected melon plants were employed to examine sucrose transporter activity in planta. Pretreatment with PCMBS inhibited loading of newly fixed ¹⁴CO₂ into minor veins of CMV-infected plants. Moreover, CMV infection caused significant increase in CmSUT1 transcripts expression, mainly in vascular bundles of minor veins, which was associated with elevated sucrose content in phloem sap collected from source-leaf petioles. We propose that cucurbit plants contain the machinery for apoplastic phloem loading and that CMV infection causes a quantitative shift in the mode by which photoassimilates are loaded into the sieve tube.
Bibliography:	http://dx.doi.org/10.1111/j.1365-313X.2011.04498.x ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0960-7412 1365-313X
DOI:	10.1111/j.1365-313X.2011.04498.x