Influence of the sterilization technic and culture media components on the growth and dissociation of Brucella abortus strain 19 in submerged cultures

Submerged cultures of Brucella abortus strain 19 were studied in shaking flasks. The influence of the sterilization methods and the medium composition on the bacterial yield and cellular dissociation were studied. The selected medium was as follows (amounts in g/l): casein pancreatic hydrolizated 30...

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Bibliographic Details
Published in:	Revista de la Asociación Argentina de Microbiología Vol. 10; no. 3; p. 83
Main Authors:	Yantorno, O Y, Mazza, L A, Balatti, A P, Aguirre, W
Format:	Journal Article
Language:	Spanish
Published:	Argentina 01-09-1978
Subjects:	Bacteriological Techniques Brucella abortus - growth & development Brucella abortus - isolation & purification Brucella Vaccine - standards Cell Fractionation Culture Media Glucose Glycerol Nitrogen Peptones Sterilization - methods
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Summary:	Submerged cultures of Brucella abortus strain 19 were studied in shaking flasks. The influence of the sterilization methods and the medium composition on the bacterial yield and cellular dissociation were studied. The selected medium was as follows (amounts in g/l): casein pancreatic hydrolizated 30; yeast extract 10; glucose, 30; sodium phosphate dibasic anhydrous 3,3; sodium monobasic monohydrate 9. Cell concentration of 8 . 10(10) viable cell/ml was obtained after 48 hours when the medium components were separated and sterilized at 121 degrees C for 20 min in autoclave.
ISSN:	0325-1713