Expression of Antioxidant Molecules and Heat Shock Protein 27 in Thyroid Tumors

ABSTRACT Oxidative stress‐induced DNA damage is a known causing factor for many types of tumors, but information on the role of oxidants and antioxidants in thyroid tumors is limited. The aim of this study was to determine antioxidant levels in thyroid tumors. In this study, tumor and its matched no...

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Published in:	Journal of cellular biochemistry Vol. 117; no. 11; pp. 2473 - 2481
Main Authors:	Wang, Shanshan, Yang, Shucui, Vlantis, Alexander C., Liu, Shirley Y.W., Ng, Enders K.W., Chan, Amy B.W., Wu, Juekun, Du, Jing, Wei, Wei, Liu, Xiaoling, Liu, Zhimin, Ng, Siu Kwan, van Hasselt, C. Andrew, Tong, Michael C.F., Chen, George G.
Format:	Journal Article
Language:	English
Published:	United States Blackwell Publishing Ltd 01-11-2016 Wiley Subscription Services, Inc
Subjects:	27 kd HEAT-SHOCK PROTEIN Adenocarcinoma, Follicular - metabolism Adenocarcinoma, Follicular - pathology ANTIOXIDANTS Antioxidants - metabolism Biomarkers, Tumor - metabolism Carcinoma, Papillary - metabolism Carcinoma, Papillary - pathology Cells, Cultured Gene Expression Regulation, Neoplastic HSP27 Heat-Shock Proteins - metabolism Humans Immunoenzyme Techniques OXIDANTS Oxidative Stress Thyroid Gland - metabolism Thyroid Neoplasms - metabolism Thyroid Neoplasms - pathology THYROID TUMORS ANTIOXIDANTS THYROID TUMORS 27 kd HEAT-SHOCK PROTEIN OXIDANTS
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Summary:	ABSTRACT Oxidative stress‐induced DNA damage is a known causing factor for many types of tumors, but information on the role of oxidants and antioxidants in thyroid tumors is limited. The aim of this study was to determine antioxidant levels in thyroid tumors. In this study, tumor and its matched non‐tumor thyroid tissue samples were obtained from 53 patients with thyroid tumors. The levels of manganese superoxide dismutase (MnSOD), thioredoxin reductase 2 (TXNRD2), glutathione (GSH), glutathione peroxidase (Gpx), catalase (CAT), and 27 kd heat‐shock protein (hsp27) were determined in both thyroid tissue samples and cultured thyroid cells by immunohistochemical staining and western blot. Hydrogen peroxide (H2O2) was used to generate oxidant stress in the cell culture experiments. We found that the levels of MnSOD, TXNRD2, GSH, Gpx, and Hsp27 were increased in both malignant and benign tumors, while the level of CAT was decreased. To verify the results of the tissue study, we treated cultured thyroid cells with H2O2 and found the same pattern of antioxidant changes. Hsp27 was also increased after H2O2 treatment. The expression of hsp27 was upregulated by 8.24‐, 6.96‐, and 3.09‐fold in thyroid cancer, follicular adenoma, multinodular goiter, respectively. Collectively, our study demonstrated that the levels of hsp27 together with MnSOD, TXNRD2, GSH, and Gpx were significantly upregulated by H2O2 in thyroid tumors. The increase of these antioxidants is observed in both malignant and benign tumors, particularly in the former. The upregulation of antioxidants is likely a protective mechanism of tumor cells to maintain their survival and growth. J. Cell. Biochem. 117: 2473–2481, 2016. © 2016 Wiley Periodicals, Inc. The levels of hsp27 together with MnSOD, TXNRD2, GSH, and Gpx were significantly upregulated by hydrogen peroxide in thyroid tumors. The upregulation of antioxidants is likely a protective mechanism of tumor cells to maintain their survival and growth. The findings present an opportunity to develop novel therapeutics for this common tumor.
Bibliography:	istex:1E957D1F12D9D2761E307B3A98AC13177F357EBE Chinese University of Hong Kong - No. 2013.2.007 ArticleID:JCB25539 ark:/67375/WNG-XMZQB1WB-B ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0730-2312 1097-4644
DOI:	10.1002/jcb.25539