A tyrosine sulfation-dependent HLA-I modification identifies memory B cells and plasma cells

Memory B cells and plasma cells are antigen-experienced cells tasked with the maintenance of humoral protection. Despite these prominent functions, definitive cell surface markers have not been identified for these cells. We report here the isolation and characterization of the monoclonal variable l...

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Published in:	Science advances Vol. 4; no. 11; p. eaar7653
Main Authors:	Chan, Justin T H, Liu, Yanling, Khan, Srijit, St-Germain, Jonathan R, Zou, Chunxia, Leung, Leslie Y T, Yang, Judi, Shi, Mengyao, Grunebaum, Eyal, Campisi, Paolo, Propst, Evan J, Holler, Theresa, Bar-Or, Amit, Wither, Joan E, Cairo, Christopher W, Moran, Michael F, Palazzo, Alexander F, Cooper, Max D, Ehrhardt, Götz R A
Format:	Journal Article
Language:	English
Published:	United States American Association for the Advancement of Science 01-11-2018
Subjects:	Animals Antibodies, Monoclonal - blood Antibodies, Monoclonal - immunology B-Lymphocytes - immunology B-Lymphocytes - metabolism Cells, Cultured Histocompatibility Antigens Class I - immunology Histocompatibility Antigens Class I - metabolism Humans Immunoglobulin Variable Region - immunology Immunoglobulin Variable Region - metabolism Immunologic Memory - immunology Immunology Lampreys - immunology Lupus Erythematosus, Systemic - immunology Lupus Erythematosus, Systemic - metabolism Multiple Sclerosis - immunology Multiple Sclerosis - metabolism Plasma Cells - immunology Plasma Cells - metabolism Receptors, Antigen - immunology Receptors, Antigen - metabolism SciAdv r-articles Tyrosine - analogs & derivatives Tyrosine - chemistry
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Summary:	Memory B cells and plasma cells are antigen-experienced cells tasked with the maintenance of humoral protection. Despite these prominent functions, definitive cell surface markers have not been identified for these cells. We report here the isolation and characterization of the monoclonal variable lymphocyte receptor B (VLRB) N8 antibody from the evolutionarily distant sea lamprey that specifically recognizes memory B cells and plasma cells in humans. Unexpectedly, we determined that VLRB N8 recognizes the human leukocyte antigen-I (HLA-I) antigen in a tyrosine sulfation-dependent manner. Furthermore, we observed increased binding of VLRB N8 to memory B cells in individuals with autoimmune disorders multiple sclerosis and systemic lupus erythematosus. Our study indicates that lamprey VLR antibodies uniquely recognize a memory B cell- and plasma cell-specific posttranslational modification of HLA-I, the expression of which is up-regulated during B cell activation.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 These authors contributed equally to this work.
ISSN:	2375-2548 2375-2548
DOI:	10.1126/sciadv.aar7653