Nanodiamond conjugated fluorescein through ethylenediamine linker for cellular biomarking

Nanodiamond conjugated fluorescein through ethylenediamine linker for cellular biomarking

Nanodiamond (DND) has a wide range of applications in the field of biomedicine owing to its outstanding biocompatibility and low toxicity as well as optical properties. In this work, DND-fluorescein composite nanoparticles were obtained by using a novel and green strategy that is mechanochemistry ap...

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Bibliographic Details
Published in:Diamond and related materials Vol. 118; p. 108546
Main Authors: Ma, Mengdi, Guan, Pei, Mukerabigwi, Jean Felix, Yan, Faning, Chen, Didi, Sun, Yuyang, Huang, Xueying, Cao, Yu
Format: Journal Article
Language:English
Published: Amsterdam Elsevier B.V 01-10-2021
Elsevier BV
Subjects:
Aqueous solutions
Biocompatibility
Biomarker
Diamonds
Diffusion layers
Ethylenediamine
Fluorescein
Fluorescence
Medical imaging
Nanodiamond
Nanoparticles
Nanostructure
Optical properties
Skin permeation
Surface functionalization
Toxicity
Surface functionalization
Nanodiamond
Biomarker
Skin permeation
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Summary:Nanodiamond (DND) has a wide range of applications in the field of biomedicine owing to its outstanding biocompatibility and low toxicity as well as optical properties. In this work, DND-fluorescein composite nanoparticles were obtained by using a novel and green strategy that is mechanochemistry approach to achieve surface functionalization of DND with fluorescein using ethylenediamine as a linker. Particularly, our prepared DND-fluorescein composite can be dispersed stably in aqueous solution with a particle size that is about 138 nm. Moreover, the determined skin penetration property shows that, unlike free fluorescein, the fluorescent DND-fluorescein nanoparticles can only remain on the stratum corneum with no diffusion into dermal layer, and it can be easily washed off with water. DND-fluorescein particles will not affect the body and are basically non-toxic to human hepatocellular carcinoma cell line (HepG2) and human normal hepatocyte cell line (LO2) cell lines. These results suggested that DND-fluorescein can be used as long-term labelling agent with excellent properties that can be exploited for various applications such as in biolabeling and bioimaging. [Display omitted] •Has good dispersion stability•Remain on the stratum corneum•As long-term labelling agent
ISSN:0925-9635
1879-0062
DOI:10.1016/j.diamond.2021.108546