High-Performance Liquid Chromatography Determination of Carvedilol in Pig Serum

A simple and sensitive analytical method for quantification of carvedilol in pig serum was developed and validated. Carvedilol and internal standard (IS) were extracted into n-hexane-dichloromethane solvent system and separated using an isocratic mobile phase on a Phenomenex C18 column. The eluent w...

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Bibliographic Details
Published in:	Journal of chromatographic science Vol. 48; no. 5; pp. 348 - 352
Main Authors:	Yamsani, Vamshi Vishnu, Gannu, Ramesh, Yamsani, Madhsudan Rao, Veerabrahma, Kishan
Format:	Journal Article
Language:	English
Published:	Niles, IL Oxford University Press 01-05-2010 Preston Publications
Subjects:	Analysis Animals Antihypertensive Agents - blood Antihypertensive Agents - pharmacokinetics Biological and medical sciences Carbazoles - blood Carbazoles - pharmacokinetics Carvedilol Chromatography, High Pressure Liquid - methods General pharmacology Male Medical sciences Pharmacology. Drug treatments Propanolamines - blood Propanolamines - pharmacokinetics Swine Biological fluid Chemical analysis Vasodilator agent α1-Adrenergic receptor HPLC chromatography β2-Adrenergic receptor Blood Alpha blocking agent β1-Adrenergic receptor Hydroxyether Antagonist Ungulata Quantitative analysis Validation Fluorescence detector Aminoalcohol Carbazole derivatives Oral administration Pig Vertebrata Reversed phase chromatography Mammalia Animal Carvedilol Antihypertensive agent Artiodactyla Pharmacokinetics Blood serum Beta blocking agent
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Summary:	A simple and sensitive analytical method for quantification of carvedilol in pig serum was developed and validated. Carvedilol and internal standard (IS) were extracted into n-hexane-dichloromethane solvent system and separated using an isocratic mobile phase on a Phenomenex C18 column. The eluent was monitored by spectroflourimetric detector at a flow rate of 1.0 mL/min. The linearity range of proposed method was 1-1000 ng/mL. The intra-day and inter-day coefficient of variation and percent error values of the assay method were less than 15%, and mean recovery was more than 89.95 and 94.27 for carvedilol and IS, respectively. The method is applicable for use in the pharmacokinetic characterization of carvedilol after administration of buccal patch (6.25 mg) in pigs.
Bibliography:	istex:92D15C01D78883E3C6740946C47F31EBE87F5178 ark:/67375/HXZ-3J8BLV1T-X ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0021-9665 1945-239X
DOI:	10.1093/chromsci/48.5.348