Perchloric acid-soluble protein regulates cell proliferation and differentiation in the spinal cord of chick embryos

The role of perchloric acid-soluble protein (PSP) was investigated in chick embryos. Fluorescently labeled anti-chick liver (CL)-PSP IgG was injected into the yolk sac in ovo at embryonic day 3, and became localized in neuroepithelial cells. Within 12h, morphological changes were observed in 37.5% o...

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Bibliographic Details
Published in:	FEBS letters Vol. 579; no. 11; pp. 2416 - 2420
Main Authors:	Himeno, Emi, Yamazaki, Kunitaka, Kanouchi, Hiroaki, Matsumoto, Mitsuharu, Sugimoto, Yasushi, Oka, Tatsuzo
Format:	Journal Article
Language:	English
Published:	England Elsevier B.V 25-04-2005
Subjects:	Animals Cell differentiation Cell Differentiation - drug effects Cell proliferation Cell Proliferation - drug effects Chick Embryo Gene Expression Regulation, Developmental Heat-Shock Proteins - antagonists & inhibitors Heat-Shock Proteins - immunology Heat-Shock Proteins - metabolism Immunoglobulin G - immunology Immunoglobulin G - pharmacology Perchlorates - pharmacology Perchloric acid-soluble protein (PSP) Ribonucleases - antagonists & inhibitors Ribonucleases - immunology Ribonucleases - metabolism Solubility - drug effects Spinal cord Spinal Cord - cytology Spinal Cord - drug effects Spinal Cord - embryology Spinal Cord - metabolism Cell proliferation Chick embryo Spinal cord Cell differentiation Perchloric acid-soluble protein (PSP)
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Summary:	The role of perchloric acid-soluble protein (PSP) was investigated in chick embryos. Fluorescently labeled anti-chick liver (CL)-PSP IgG was injected into the yolk sac in ovo at embryonic day 3, and became localized in neuroepithelial cells. Within 12h, morphological changes were observed in 37.5% of anti-CL-PSP IgG-injected embryos, and the neuroepithelial cells formed a wavy line. No significant changes were observed in embryos injected with non-immune IgG or PBS. Increased expression of PCNA and decreased expression of neuronal class III β-tubulin were observed in the spinal cord after anti-CL-PSP IgG injection. These results suggest that PSP controls the proliferation and differentiation of neuroepithelial cells in chick embryos.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0014-5793 1873-3468
DOI:	10.1016/j.febslet.2005.02.081