Search Results - "Yamauchi, Kevin"

Single cell–resolution western blotting by Kang, Chi-Chih, Yamauchi, Kevin A, Vlassakis, Julea, Sinkala, Elly, Duncombe, Todd A, Herr, Amy E

“…Single-cell-resolution western blotting concatenates an electrophoretic separation to downstream immunoprobing of unfixed mammalian cells to detect proteins,…”
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Profiling protein expression in circulating tumour cells using microfluidic western blotting by Sinkala, Elly, Sollier-Christen, Elodie, Renier, Corinne, Rosàs-Canyelles, Elisabet, Che, James, Heirich, Kyra, Duncombe, Todd A., Vlassakis, Julea, Yamauchi, Kevin A., Huang, Haiyan, Jeffrey, Stefanie S., Herr, Amy E.

“…Circulating tumour cells (CTCs) are rare tumour cells found in the circulatory system of certain cancer patients. The clinical and functional significance of…”
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Therapy-Induced Evolution of Human Lung Cancer Revealed by Single-Cell RNA Sequencing by Maynard, Ashley, McCoach, Caroline E., Rotow, Julia K., Harris, Lincoln, Haderk, Franziska, Kerr, D. Lucas, Yu, Elizabeth A., Schenk, Erin L., Tan, Weilun, Zee, Alexander, Tan, Michelle, Gui, Philippe, Lea, Tasha, Wu, Wei, Urisman, Anatoly, Jones, Kirk, Sit, Rene, Kolli, Pallav K., Seeley, Eric, Gesthalter, Yaron, Le, Daniel D., Yamauchi, Kevin A., Naeger, David M., Bandyopadhyay, Sourav, Shah, Khyati, Cech, Lauren, Thomas, Nicholas J., Gupta, Anshal, Gonzalez, Mayra, Do, Hien, Tan, Lisa, Bacaltos, Bianca, Gomez-Sjoberg, Rafael, Gubens, Matthew, Jahan, Thierry, Kratz, Johannes R., Jablons, David, Neff, Norma, Doebele, Robert C., Weissman, Jonathan, Blakely, Collin M., Darmanis, Spyros, Bivona, Trever G.

“…Lung cancer, the leading cause of cancer mortality, exhibits heterogeneity that enables adaptability, limits therapeutic success, and remains incompletely…”
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Controlling Dispersion during Single-Cell Polyacrylamide-Gel Electrophoresis in Open Microfluidic Devices by Pan, Qiong, Yamauchi, Kevin A, Herr, Amy E

“…New tools for measuring protein expression in individual cells complement single-cell genomics and transcriptomics. To characterize a population of individual…”
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Laterally Aggregated Polyacrylamide Gels for Immunoprobed Isoelectric Focusing by Jeeawoody, Shaheen, Yamauchi, Kevin A, Su, Alison, Herr, Amy E

“…Immunoprobed isoelectric focusing (IEF) resolves proteins based on differences in isoelectric point (pI) and then identifies protein targets through…”
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Detection of Isoforms Differing by a Single Charge Unit in Individual Cells by Tentori, Augusto M., Yamauchi, Kevin A., Herr, Amy E.

“…To measure protein isoforms in individual mammalian cells, we report single‐cell resolution isoelectric focusing (scIEF) and high‐selectivity immunoprobing…”
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SpatialData: an open and universal data framework for spatial omics by Marconato, Luca, Palla, Giovanni, Yamauchi, Kevin A, Virshup, Isaac, Heidari, Elyas, Treis, Tim, Vierdag, Wouter-Michiel, Toth, Marcella, Stockhaus, Sonja, Shrestha, Rahul B, Rombaut, Benjamin, Pollaris, Lotte, Lehner, Laurens, Vöhringer, Harald, Kats, Ilia, Saeys, Yvan, Saka, Sinem K, Huber, Wolfgang, Gerstung, Moritz, Moore, Josh, Theis, Fabian J, Stegle, Oliver

“…Spatially resolved omics technologies are transforming our understanding of biological tissues. However, the handling of uni- and multimodal spatial omics…”
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Validation and tuning of in situ transcriptomics image processing workflows with crowdsourced annotations by Vo-Phamhi, Jenny M, Yamauchi, Kevin A, Gómez-Sjöberg, Rafael

“…Recent advancements in in situ methods, such as multiplexed in situ RNA hybridization and in situ RNA sequencing, have deepened our understanding of the way…”
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Fabrication of an Open Microfluidic Device for Immunoblotting by Abdel-Sayed, Philippe, Yamauchi, Kevin A, Gerver, Rachel E, Herr, Amy E

“…Given the wide adoption of polydimethylsiloxane (PDMS) for the rapid fabrication of microfluidic networks and the utility of polyacrylamide gel electrophoresis…”
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Subcellular western blotting of single cells by Yamauchi, Kevin A., Herr, Amy E.

“…Although immunoassays are the de facto standard for determining subcellular protein localization in individual cells, antibody probe cross-reactivity and…”
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Arrayed isoelectric focusing using photopatterned multi‐domain hydrogels by Yamauchi, Kevin A., Tentori, Augusto M., Herr, Amy E.

“…Isoelectric focusing (IEF) is a powerful separation method, useful for resolving subtle changes in the isoelectric point of unlabeled proteins. While…”
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OME-Zarr: a cloud-optimized bioimaging file format with international community support by Moore, Josh, Basurto-Lozada, Daniela, Besson, Sébastien, Bogovic, John, Bragantini, Jordão, Brown, Eva M., Burel, Jean-Marie, Casas Moreno, Xavier, de Medeiros, Gustavo, Diel, Erin E., Gault, David, Ghosh, Satrajit S., Gold, Ilan, Halchenko, Yaroslav O., Hartley, Matthew, Horsfall, Dave, Keller, Mark S., Kittisopikul, Mark, Kovacs, Gabor, Küpcü Yoldaş, Aybüke, Kyoda, Koji, le Tournoulx de la Villegeorges, Albane, Li, Tong, Liberali, Prisca, Lindner, Dominik, Linkert, Melissa, Lüthi, Joel, Maitin-Shepard, Jeremy, Manz, Trevor, Marconato, Luca, McCormick, Matthew, Lange, Merlin, Mohamed, Khaled, Moore, William, Norlin, Nils, Ouyang, Wei, Özdemir, Bugra, Palla, Giovanni, Pape, Constantin, Pelkmans, Lucas, Pietzsch, Tobias, Preibisch, Stephan, Prete, Martin, Rzepka, Norman, Samee, Sameeul, Schaub, Nicholas, Sidky, Hythem, Solak, Ahmet Can, Stirling, David R., Striebel, Jonathan, Tischer, Christian, Toloudis, Daniel, Virshup, Isaac, Walczysko, Petr, Watson, Alan M., Weisbart, Erin, Wong, Frances, Yamauchi, Kevin A., Bayraktar, Omer, Cimini, Beth A., Gehlenborg, Nils, Haniffa, Muzlifah, Hotaling, Nathan, Onami, Shuichi, Royer, Loic A., Saalfeld, Stephan, Stegle, Oliver, Theis, Fabian J., Swedlow, Jason R.

“…A growing community is constructing a next-generation file format (NGFF) for bioimaging to overcome problems of scalability and heterogeneity. Organized by the…”
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ReScan, a Multiplex Diagnostic Pipeline, Pans Human Sera for SARS-CoV-2 Antigens by Zamecnik, Colin R., Rajan, Jayant V., Yamauchi, Kevin A., Mann, Sabrina A., Loudermilk, Rita P., Sowa, Gavin M., Zorn, Kelsey C., Alvarenga, Bonny D., Gaebler, Christian, Caskey, Marina, Stone, Mars, Norris, Philip J., Gu, Wei, Chiu, Charles Y., Ng, Dianna, Byrnes, James R., Zhou, Xin X., Wells, James A., Robbiani, Davide F., Nussenzweig, Michel C., DeRisi, Joseph L., Wilson, Michael R.

“…Comprehensive understanding of the serological response to SARS-CoV-2 infection is important for both pathophysiologic insight and diagnostic development…”
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Summit: Automated Analysis of Arrayed Single-Cell Gel Electrophoresis by Vlassakis, Julea, Yamauchi, Kevin A, Herr, Amy E

“…New pipelines are required to automate the quantitation of emerging high-throughput electrophoretic (EP) assessment of DNA damage, or proteoform expression in…”
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Corrigendum: Detection of Isoforms Differing by a Single Charge Unit in Individual Cells by Tentori, Augusto M., Yamauchi, Kevin A., Herr, Amy E.

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Integrating qPLM and biomechanical test data with an anisotropic fiber distribution model and predictions of TGF-1 and IGF-1 regulation of articular cartilage fiber modulus by Stender, Michael E., Raub, Christopher B., Yamauchi, Kevin A., Shirazi, Reza, Vena, Pasquale, Sah, Robert L., Hazelwood, Scott J., Klisch, Stephen M.

“…A continuum mixture model with distinct collagen (COL) and glycosaminoglycan elastic constituents was developed for the solid matrix of immature bovine…”
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Detection of Isoforms Differing by a Single Charge Unit in Individual Cells by Tentori, Augusto M., Yamauchi, Kevin A., Herr, Amy E.

“…To measure protein isoforms in individual mammalian cells, we report single‐cell resolution isoelectric focusing (scIEF) and high‐selectivity immunoprobing…”
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Integrating qPLM and biomechanical test data with an anisotropic fiber distribution model and predictions of TGF-β1 and IGF-1 regulation of articular cartilage fiber modulus by Stender, Michael E, Raub, Christopher B, Yamauchi, Kevin A, Shirazi, Reza, Vena, Pasquale, Sah, Robert L, Hazelwood, Scott J, Klisch, Stephen M

“…A continuum mixture model with distinct collagen (COL) and glycosaminoglycan elastic constituents was developed for the solid matrix of immature bovine…”
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Berichtigung: Detection of Isoforms Differing by a Single Charge Unit in Individual Cells by Tentori, Augusto M., Yamauchi, Kevin A., Herr, Amy E.

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Ageing hallmarks exhibit organ-specific temporal signatures by Schaum, Nicholas, Lehallier, Benoit, Hahn, Oliver, Pálovics, Róbert, Hosseinzadeh, Shayan, Lee, Song E., Sit, Rene, Lee, Davis P., Losada, Patricia Morán, Zardeneta, Macy E., Fehlmann, Tobias, Webber, James T., McGeever, Aaron, Calcuttawala, Kruti, Zhang, Hui, Berdnik, Daniela, Mathur, Vidhu, Tan, Weilun, Zee, Alexander, Tan, Michelle, Pisco, Angela Oliveira, Karkanias, Jim, Neff, Norma F., Keller, Andreas, Darmanis, Spyros, Quake, Stephen R., Wyss-Coray, Tony

“…Ageing is the single greatest cause of disease and death worldwide, and understanding the associated processes could vastly improve quality of life. Although…”
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