B1500, a small membrane protein, connects the two-component systems EvgS/EvgA and PhoQ/PhoP in Escherichia coli

Two-component signal-transduction systems (TCSs) of bacteria are considered to form an intricate signal network to cope with various environmental stresses. One example of such a network in Escherichia coli is the signal transduction cascade from the EvgS/EvgA system to the PhoQ/PhoP system, where a...

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Published in:	Proceedings of the National Academy of Sciences - PNAS Vol. 104; no. 47; pp. 18712 - 18717
Main Authors:	Eguchi, Yoko, Itou, Junji, Yamane, Masatake, Demizu, Ryo, Yamato, Fumiyuki, Okada, Ario, Mori, Hirotada, Kato, Akinori, Utsumi, Ryutaro
Format:	Journal Article
Language:	English
Published:	United States National Academy of Sciences 20-11-2007 National Acad Sciences
Subjects:	Amino Acid Sequence Antibodies Bacterial Proteins - genetics Bacterial Proteins - metabolism Base Sequence Biological Sciences Cell Membrane - metabolism Cloning, Molecular DNA E coli Escherichia coli Escherichia coli Proteins - genetics Escherichia coli Proteins - metabolism Gene expression Gene expression regulation Gene Expression Regulation, Bacterial Genes Membrane proteins Membrane Proteins - genetics Membrane Proteins - metabolism Membranes Molecular Sequence Data Open reading frames Plasmids Protein Binding Protein Kinases - genetics Protein Kinases - metabolism Proteins Sensors Signal Transduction Transcription Factors - genetics Transcription Factors - metabolism
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Summary:	Two-component signal-transduction systems (TCSs) of bacteria are considered to form an intricate signal network to cope with various environmental stresses. One example of such a network in Escherichia coli is the signal transduction cascade from the EvgS/EvgA system to the PhoQ/PhoP system, where activation of the EvgS/EvgA system promotes expression of PhoP-activated genes. As a factor connecting this signal transduction cascade, we have identified a small inner membrane protein (65 aa), B1500. Expression of the b1500 gene is directly regulated by the EvgS/EvgA system, and b1500 expression from a heterologous promoter simultaneously activated the expression of mgtA and other PhoP regulon genes. This activation was PhoQ/PhoP-dependent and EvgS/EvgA-independent. Furthermore, deletion of b1500 from an EvgS-activated strain suppressed mgtA expression. B1500 is localized in the inner membrane, and bacterial two-hybrid data showed that B1500 formed a complex with the sensor PhoQ. These results indicate that the small membrane protein, B1500, connected the signal transduction between EvgS/EvgA and PhoQ/PhoP systems by directly interacting with PhoQ, thus activating the PhoQ/PhoP system.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Author contributions: Y.E. and J.I. contributed equally to this work; R.U. designed research; Y.E., J.I., M.Y., R.D., F.Y., A.O., H.M., and A.K. performed research; and Y.E. and R.U. wrote the paper. Edited by Eduardo A. Groisman, Washington University, St. Louis, MO, and accepted by the Editorial Board October 3, 2007
ISSN:	0027-8424 1091-6490
DOI:	10.1073/pnas.0705768104