Healthy births and ongoing pregnancies obtained by preimplantation genetic diagnosis in patients with advanced maternal age and recurrent implantation failure

Healthy births and ongoing pregnancies obtained by preimplantation genetic diagnosis in patients with advanced maternal age and recurrent implantation failure

Preimplantation genetic diagnosis (PGD) and subsequent embryo development was evaluated in 72 couples presenting at our centre for intracytoplasmic sperm injection (ICSI) due to severe male factor. The embryo biopsies were performed in Ca2+/Mg2+-free medium. These patients were further divided into...

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Bibliographic Details
Published in:Human reproduction (Oxford) Vol. 15; no. 9; pp. 2003 - 2007
Main Authors: Kahraman, S., Bahçe, M., Şamlı, H., İmirzalıoğlu, N., Yakısn, K., Cengiz, G., Dönmez, E.
Format: Journal Article
Language:English
Published: Oxford Oxford University Press 01-09-2000
Subjects:
Adult
Aneuploidy
Biological and medical sciences
Biopsy
Blastomeres - ultrastructure
Buffers
Ca2
Calcium
Chromosome Aberrations
chromosomes X
Embryo Implantation
Embryo Transfer
Female
FISH
Fundamental and applied biological sciences. Psychology
Humans
In Situ Hybridization, Fluorescence
Indicators and Reagents
Infertility, Male - therapy
Magnesium
Male
Maternal Age
Mg2+-free medium
Mother. Fetoplacental unit. Mammary gland. Milk
Pregnancy
Pregnancy Outcome
Pregnancy, High-Risk
Pregnancy. Parturition. Lactation
Preimplantation Diagnosis
preimplantation genetic diagnosis
Sperm Injections, Intracytoplasmic
Vertebrates: reproduction
chromosomes X, Y, 13, 18, 21
aneuploidy
FISH
free medium
Mg
preimplantation genetic diagnosis
Ca
Pregnancy
Chromosomal aberration
Human
Preimplantation diagnosis
Embryonic development
Maternal age
Ovum implantation
Medium without
Aneuploidy
Female
Magnesium Cations
Calcium Cations
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Summary:Preimplantation genetic diagnosis (PGD) and subsequent embryo development was evaluated in 72 couples presenting at our centre for intracytoplasmic sperm injection (ICSI) due to severe male factor. The embryo biopsies were performed in Ca2+/Mg2+-free medium. These patients were further divided into those with advanced maternal age (AMA, n = 49) and those with recurrent implantation failure (RIF, n = 23). Fluorescence in-situ hybridization (FISH) was carried out on 329 blastomeres (91.3%) with probes for the X, Y, 13, 18 and 21 chromosomes. The chromosomal abnormality rate was 41.3% with no significant difference between the AMA and RIF groups. Aneuploidy accounted for the majority (72.8%) of chromosomal abnormalities. Out of 329 embryos, 84.2% had cleaved after 24 h and 15.1% had arrested. Embryos were transferred in 70 patients and 22 pregnancies were achieved (31.4% with an ongoing pregnancy rate of 28.5%). There were no significant differences between the pregnancy rates of the AMA and RIF groups (32.5 and 30% respectively). Therefore PGD should be offered to patients with AMA and RIF. Furthermore, the use of Ca2+/Mg2+-free medium during the blastomere biopsy facilitates the procedure, while further embryo cleavage, ongoing pregnancies and healthy births are possible.
Bibliography:istex:17388CE8FD16D40C0E731EA0BD4037FF7C1207DC
local:0152003
ark:/67375/HXZ-PW4H003J-S
PII:1460-2350
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0268-1161
1460-2350
DOI:10.1093/humrep/15.9.2003