The Effect of miR-98 and miR-214 on Apoptotic and Angiogenic Pathways in Hepatocellular Carcinoma HepG2 Cells

The Effect of miR-98 and miR-214 on Apoptotic and Angiogenic Pathways in Hepatocellular Carcinoma HepG2 Cells

Hepatocellular carcinoma (HCC) is one of the foremost causes of cancer related morbidity worldwide. An increasing number of studies have confirmed that microRNAs play an important role in the development, progression and metastasis of HCC. From those important miRNAs are miR-98 and miR-214. This stu...

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Bibliographic Details
Published in:Indian journal of clinical biochemistry Vol. 35; no. 3; pp. 353 - 358
Main Authors: Yahya, Shaymaa M. M., Yahya, Shereen M. M.
Format: Journal Article
Language:English
Published: New Delhi Springer India 01-07-2020
Springer
Springer Nature B.V
Subjects:
Analysis
Angiogenesis
Apoptosis
Bcl-2 protein
Biochemistry
Biomedical and Life Sciences
Cdc2 protein
Chemistry/Food Science
Ethylenediaminetetraacetic acid
Fibroblast growth factor 1
Gelatinase A
Gelatinase B
Gene expression
Genes
Genetic research
Health aspects
Hepatocellular carcinoma
Hepatoma
Life Sciences
Liver cancer
Metastases
Metastasis
Microbiology
MicroRNA
MicroRNAs
miRNA
Morbidity
Original
Original Research Article
p53 Protein
Pathology
Survivin
Transfection
Tumor proteins
Vascular endothelial growth factor
Angiogenesis
Hepatocellular carcinoma
miR-214
miR-98
Apoptosis
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Summary:Hepatocellular carcinoma (HCC) is one of the foremost causes of cancer related morbidity worldwide. An increasing number of studies have confirmed that microRNAs play an important role in the development, progression and metastasis of HCC. From those important miRNAs are miR-98 and miR-214. This study were conducted to explore the effect of these two miRNAs on some apoptotic and angiogenic genes namely, BCL-2, survivin, CCND1, CDC2, P53 and P21, VEGF, Hif-1α, MMP-2, MMP-9, Ang-1, Ang-2, and FGF-1. miRNAs mimics and inhibitors transfection was used to investigate the role of both studied molecules in apoptosis and angiogenesis in HepG2 cells. QRT-PCR was used for Quantitative gene and miRNA expression analyses. The study revealed that miR-98 could serve as a pro-apoptotic factor through the upregulation of P53 gene expression levels. Besides, the anti-angiogenic effect of this miRNA was evident through the down regulation of Ang-1 and FGF-1 genes. Meanwhile, miR-214 showed a pro-apoptotic role and anti-angiogenic effects. These effects were verified through the significant down regulation of BCL-2, CDC2, VEGF, Ang-1 and MMP-2. These results introduced a possible positive role played by both miR-98 and miR-214 on some pro-apoptotic and anti-angiogenic genes.
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ISSN:0970-1915
0974-0422
DOI:10.1007/s12291-019-00824-1