Cytochrome P450 and Glutathione-S-Transferase Activity are Altered Following Environmentally Relevant Atrazine Exposures in Crayfish (Faxoniusvirilis)

Cytochrome P450 and Glutathione-S-Transferase Activity are Altered Following Environmentally Relevant Atrazine Exposures in Crayfish (Faxoniusvirilis)

The herbicide atrazine is heavily applied in the U.S. Midwest to control broadleaf weeds. It enters local streams and rivers through runoff and seepage, and exposure can affect non-target aquatic organisms, like crayfish. We examined sublethal effects of atrazine on the expression and activity of th...

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Bibliographic Details
Published in:Bulletin of environmental contamination and toxicology Vol. 103; no. 4; pp. 579 - 584
Main Authors: Awali, Saamera, Abdulelah, Sara A., Crile, Karen G., Yacoo, Kathrine E., Almouseli, Abdrhman, Torres, Victoria C., Dayfield, Daniel J., Evans, Kendra R., Belanger, Rachelle M.
Format: Journal Article
Language:English
Published: New York Springer US 01-10-2019
Springer Nature B.V
Subjects:
Aquatic organisms
Aquatic Pollution
Atrazine
Crayfish
Cytochrome
Cytochrome P450
Cytochromes P450
Detoxification
Earth and Environmental Science
Ecotoxicology
Environment
Environmental Chemistry
Environmental Health
Exposure
Frogs
Glutathione
Glutathione transferase
Hepatopancreas
Herbicides
Pollution
Rivers
Runoff
Seepage
Soil Science & Conservation
Sublethal effects
Waste Water Technology
Water Management
Water Pollution Control
Crayfish
Glutathione-S-transferase
Cytochrome P450
Hepatopancreas
Atrazine
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Summary:The herbicide atrazine is heavily applied in the U.S. Midwest to control broadleaf weeds. It enters local streams and rivers through runoff and seepage, and exposure can affect non-target aquatic organisms, like crayfish. We examined sublethal effects of atrazine on the expression and activity of the detoxification enzymes cytochrome P450 (CYP450) and glutathione-S-transferase (GST) in crayfish. Crayfish were exposed to 0, 10, 40, 80, 100 and 300 ppb atrazine for 1, 2, 4, 7 and 10 days. Their hepatopancreas was collected and CYP450 expression and GST activity was analyzed. Atrazine exposure caused differential expression and activity of CYP450 and GST. CYP450 expression varied over exposure concentrations and time. Further, GST activity significantly increased following a 2 day, 10 ppb exposure to atrazine and a 300 ppb atrazine exposure for all days tested. We found that atrazine detoxification is a dynamic process that changes with the length and intensity of atrazine exposure.
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ISSN:0007-4861
1432-0800
DOI:10.1007/s00128-019-02674-2