Cloning of a new antenna gene cluster and expression analysis of the antenna gene family of Rhodopseudomonas palustris

The genome of Rhodopseudomonas palustris contains five antenna gene clusters, αβa, αβb, αβc, αβd and αβe, which encode the light‐harvesting peripheral antenna complex II polypeptides. The isolation and characterisation of the gene which encodes the αe and βe polypeptides are reported. The primary st...

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Bibliographic Details
Published in:	European journal of biochemistry Vol. 217; no. 3; pp. 867 - 875
Main Authors:	TADROS, Monier H., KATSIOU, Eleni, HOON, Mark A., YURKOVA, Natalie, RAMJI, Dipak P.
Format:	Journal Article
Language:	English
Published:	Oxford, UK Blackwell Publishing Ltd 01-11-1993 Blackwell
Subjects:	Amino Acid Sequence Bacteriology Base Sequence Biological and medical sciences Blotting, Northern Cloning, Molecular DNA, Bacterial Fundamental and applied biological sciences. Psychology Genes, Bacterial Genetics Microbiology Molecular Sequence Data Multigene Family Photosynthetic Reaction Center Complex Proteins - genetics Promoter Regions, Genetic Rhodopseudomonas - genetics Rhodopseudomonas palustris RNA - metabolism Sequence Analysis Sequence Homology, Amino Acid Spectrum Analysis Light effect Gene cluster Light harvesting system Nucleotide sequence Transcription promoter Rhodopseudomonas palustris Rhodospirillaceae Gene expression Subunit Rhodospirillales Gene Restriction map Bacteria Photosynthesis Aminoacid sequence
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Summary:	The genome of Rhodopseudomonas palustris contains five antenna gene clusters, αβa, αβb, αβc, αβd and αβe, which encode the light‐harvesting peripheral antenna complex II polypeptides. The isolation and characterisation of the gene which encodes the αe and βe polypeptides are reported. The primary structure of the βe polypeptide is identical to that of βb whilst the structure of αe is different from the other α subunits so far characterised. All five of the gene clusters were transcribed under high‐light conditions while under low‐light conditions only three were transcribed (αβb, αβd and αβe). Furthermore, Northern‐blot analysis showed that the gene clusters encode RNA transcripts of either 500 or 650 nucleotides. Individual members of the gene family showed a differential response in terms of the regulation of abundance of mRNA upon growth under either high‐light or low‐light intensities. Possible promoter sequences and operator sites upstream of the αβb, αβd and αβe genes were located. Furthermore using puc‐lacZ fusions in trans in R. palustris, we were able to examine the positions of the promoter of the gene clusters. The significance of these observations with respect to the regulation, organization and role of the peripheral antenna is discussed.
Bibliography:	The novel nucleotide sequence data published here have been submitted to the EMBL/GenBank/DNA Data Bank of Japan under the accession number X63347. Note. ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0014-2956 1432-1033
DOI:	10.1111/j.1432-1033.1993.tb18315.x