Tetrandrine inhibits hepatocellular carcinoma cell growth through the caspase pathway and G2/M phase

Activation of p53-independent pathways plays an important role in phytochemical-induced apoptosis and is considered to be a crucial factor in the invasion and metastasis of cancer. Previous studies have shown that combined effects of Stephania tetrandra with medicinal herbs exhibit beneficial effect...

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Bibliographic Details
Published in:	Oncology reports Vol. 29; no. 6; pp. 2205 - 2210
Main Authors:	YU, VIVIAN W.L, HO, WING SHING
Format:	Journal Article
Language:	English
Published:	Greece D.A. Spandidos 01-06-2013 Spandidos Publications UK Ltd
Subjects:	Antineoplastic Agents, Phytogenic - pharmacology Apoptosis Benzylisoquinolines - pharmacology cancer Cancer therapies Carcinoma, Hepatocellular - drug therapy Caspase 9 - metabolism cell cycle Cell growth Cell Line, Tumor - drug effects Cell Survival - drug effects Cyclin-Dependent Kinase Inhibitor p21 - metabolism Enzyme Activation - drug effects G2 Phase Cell Cycle Checkpoints - drug effects Huh-7 Humans inhibition Inhibitory Concentration 50 Liver cancer Liver Neoplasms - drug therapy Signal Transduction tetrandrine
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Summary:	Activation of p53-independent pathways plays an important role in phytochemical-induced apoptosis and is considered to be a crucial factor in the invasion and metastasis of cancer. Previous studies have shown that combined effects of Stephania tetrandra with medicinal herbs exhibit beneficial effects in cancer patients. Tetrandrine, an active component of Stephania tetrandra has been reported to have anticancer properties in cancer cells. However, the mechanism(s) of action of tetrandrine in liver cancer have yet to be fully elucidated. In this study, we investigated the effects of tetrandrine in hepatocellular carcinoma (HCC) cells. The results showed that tetrandrine inhibited HCC cell proliferation by suppression of cell cycle progression at the G2/M phase. Changes in the expression levels of Bax, Bcl, p53, survivin, PCNA, PARP and p21 were observed. In addition, tetrandrine increased caspase-3 expression and induced DNA fragmentation in Huh-7 cells. The results suggest that the anti-cancer effect of tetrandrine in Huh-7 cells may be mediated by p53-independent pathways.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1021-335X 1791-2431
DOI:	10.3892/or.2013.2352