Search Results - "YOUNG, Lei"
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Enzymatic assembly of DNA molecules up to several hundred kilobases
Published in Nature methods (01-05-2009)“…We describe an isothermal, single-reaction method for assembling multiple overlapping DNA molecules by the concerted action of a 5′ exonuclease, a DNA…”
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Creation of a Bacterial Cell Controlled by a Chemically Synthesized Genome
Published in Science (American Association for the Advancement of Science) (02-07-2010)“…We report the design, synthesis, and assembly of the 1.08-mega-base pair Mycoplasma mycoides JCVI-syn1.0 genome starting from digitized genome sequence…”
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RecA mediates MgpB and MgpC phase and antigenic variation in Mycoplasma genitalium, but plays a minor role in DNA repair
Published in Molecular microbiology (01-08-2012)“…Summary Mycoplasma genitalium, a sexually transmitted human pathogen, encodes MgpB and MgpC adhesins that undergo phase and antigenic variation through…”
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Unusual Bacterioplankton Community Structure in Ultra-Oligotrophic Crater Lake
Published in Limnology and oceanography (01-05-2001)“…The bacterioplankton assemblage in Crater Lake, Oregon (U.S.A.), is different from communities found in other oxygenated lakes, as demonstrated by four small…”
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Development of a murine prostatespecific 2-in-1 inducible bicistronic expression vector
Published in Biotechnology letters (01-06-2005)“…Transgenic research often suffers from the lack of strong tissue specific promoters and the lack of suitable antibodies for transgene detection. We have now…”
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Cloning of TC-1 (C8orf4), a Novel Gene Found to Be Overexpressed in Thyroid Cancer
Published in Genomics (San Diego, Calif.) (01-11-2000)“…A novel gene highly expressed in thyroid cancer, designated TC-1 (thyroid cancer-1), was cloned from suppression subtractive hybridization between papillary…”
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Complete Chemical Synthesis, Assembly, and Cloning of a Mycoplasma genitalium Genome
Published in Science (American Association for the Advancement of Science) (29-02-2008)“…We have synthesized a 582,970-base pair Mycoplasma genitalium genome. This synthetic genome, named M. genitalium JCVI-1.0, contains all the genes of wild-type…”
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Two‐step total gene synthesis method
Published in Nucleic acids research (2004)“…In the post‐genomic era, the ability to synthesize any arbitrary DNA sequence is increasingly in demand. A bottleneck in current gene synthesis technologies is…”
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Assembly of Large, High G+C Bacterial DNA Fragments in Yeast
Published in ACS synthetic biology (20-07-2012)“…The ability to assemble large pieces of prokaryotic DNA by yeast recombination has great application in synthetic biology, but cloning large pieces of high G+C…”
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Development of a murine prostate-specific 2-in-1 inducible bicistronic expression vector
Published in Biotechnology letters (01-06-2005)“…Transgenic research often suffers from the lack of strong tissue specific promoters and the lack of suitable antibodies for transgene detection. We have now…”
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Probasin Promoter-driven Expression of ID1 Is not Sufficient for Carcinogenesis in Rodent Prostate
Published in The journal of histochemistry and cytochemistry (01-06-2009)“…Inhibitor of DNA-binding-1 (ID1) negatively regulates cell differentiation and senescence, and enhances cellular proliferation and angiogenesis. Elevated…”
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TC-1 is a novel tumorigenic and natively disordered protein associated with thyroid cancer
Published in Cancer research (Chicago, Ill.) (15-04-2004)“…A novel gene, thyroid cancer 1 (TC-1), was found recently to be overexpressed in thyroid cancer. TC-1 shows no homology to any of the known thyroid…”
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Creation of a Bacterial Cell Controlled by a Chemically Synthesized
Published in Science (American Association for the Advancement of Science) (02-07-2010)“…We report the design, synthesis, and assembly of the 1.08-mega-base pair…”
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Ornithine Decarboxylase (ODC) Expression Pattern in Human Prostate Tissues and ODC Transgenic Mice
Published in The journal of histochemistry and cytochemistry (01-02-2006)“…Ornithine decarboxylase (ODC) is the key enzyme in the polyamine synthesis pathway and is overexpressed in a variety of cancers. We have performed a detailed…”
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Targeted amplification of mutant strands for efficient site-directed mutagenesis and mutant screening
Published in Methods in molecular biology (Clifton, N.J.) (2010)“…Site-directed mutagenesis is an invaluable tool for functional studies and genetic engineering. However, most current protocols require the target DNA to be…”
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TAMS technology for simple and efficient in vitro site‐directed mutagenesis and mutant screening
Published in Nucleic acids research (01-02-2003)“…Site‐directed mutagenesis is an invaluable tool for functional studies and genetic engineering. However, most current protocols require the target DNA to be…”
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Molecular cloning and characterization of a novel androgen repressible gene expressed in the prostate epithelium
Published in Gene (28-03-2005)“…Prostate cancer deaths are due to functional escape of prostate cancer cells from their original androgen-dependent growth. To better understand the origin and…”
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Prostate Epithelial Expression of a Novel Androgen Target Gene
Published in Journal of andrology (01-09-2002)“…ABSTRACT To better understand the role of androgens in prostate development and disease it is important to characterize androgen‐regulated genes in the…”
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Genomic change in hepatitis B virus associated with development of hepatocellular carcinoma
Published in World journal of gastroenterology : WJG (21-06-2016)“…AIM: To determine the genomic changes in hepatitis B virus(HBV) and evaluate their role in the development of hepatocellular carcinoma(HCC) in patients…”
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