Association of interleukin-1 beta (+3954) gene polymorphism and gingival crevicular fluid levels in patients with aggressive and chronic periodontitis

The immune mechanisms and genetic variations that regulate genetic expression, production and biological activity of IL-1beta, are thought to play an important role in the pathogenesis of periodontal disease. The aims of the present study were to analyse interleukin (IL)-1beta (+3954) genotype and a...

Full description

Saved in:

Bibliographic Details
Published in:	Genetic counseling Vol. 24; no. 1; p. 21
Main Authors:	Yücel, O Ozer, Berker, E, Mescil, L, Eratalay, K, Tepe, E, Tezcan, I
Format:	Journal Article
Language:	English
Published:	Switzerland 2013
Subjects:	Adolescent Adult Aggressive Periodontitis - genetics Aggressive Periodontitis - metabolism Analysis of Variance Chronic Periodontitis - genetics Chronic Periodontitis - metabolism Enzyme-Linked Immunosorbent Assay - methods Female Gene Frequency - genetics Gingival Crevicular Fluid - metabolism Humans Interleukin-1beta - genetics Male Middle Aged Periodontal Index Polymerase Chain Reaction - methods Polymorphism, Genetic - genetics Polymorphism, Restriction Fragment Length - genetics Polymorphism, Single Nucleotide - genetics Severity of Illness Index Turkey Young Adult Turkey
Online Access:	Get more information
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	The immune mechanisms and genetic variations that regulate genetic expression, production and biological activity of IL-1beta, are thought to play an important role in the pathogenesis of periodontal disease. The aims of the present study were to analyse interleukin (IL)-1beta (+3954) genotype and allele frequency in both chronic and aggressive periodontitis patients, and also to investigate whether this polymorphism is associated with gingival crevicular fluid (GCF) IL-1beta levels, periodontal disease severity and clinical parameters in subjects of Turkish origin. A total of 147 individuals were enrolled in the study including 56 aggressive periodontitis (AP), 44 chronic periodontitis (CP) patients and 47 healthy controls (C). Single nucleotide polymorphism at IL-1beta (+3954) is analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). GCF samples were analyzed for IL-1beta, using enzyme linked immunosorbent assay (ELISA). The distribution of genotypes and allele frequencies for IL-1beta (+3954) were similar among the groups, in spite of a trend toward a higher frequency of allele 2 in the patient groups. The genotype distribution and allele frequencies were also not different after stratification of subjects according to the clinical attachment level (CAL < 4 mm and CAL > 4mm). No differences were found between the GCF IL-1beta levels of the different genotypes. Allele 2 was associated with increased bleeding on probing (BOP) sites in chronic periodontitis patients. The results of this study do not support that genetic polymorphism in the IL-1beta (+3954) could be identified as a susceptibility or severity factor in aggressive periodontitis, in the present population. The association of allele 2 frequency and higher percentage of BOP sites in chronic periodontitis suggest that IL-1beta (+3954) potentially play a significant but not major role in the clinical outcome.
ISSN:	1015-8146