Orally delivered foot-and-mouth disease virus capsid protomer vaccine displayed on T4 bacteriophage surface: 100% protection from potency challenge in mice

Summary An orally delivered foot-and-mouth disease (FMD) vaccine has not previously been reported. By using a T4 bacteriophage nanoparticle surface gene-protein display system (T4-S-GPDS), we created a foot-and-mouth disease virus (FMDV) entire capsid protein vaccine candidate. On the T4 phage surfa...

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Bibliographic Details
Published in:	Vaccine Vol. 26; no. 11; pp. 1471 - 1481
Main Authors:	Ren, Z.J, Tian, C.J, Zhu, Q.S, Zhao, M.Y, Xin, A.G, Nie, W.X, Ling, S.R, Zhu, M.W, Wu, J.Y, Lan, H.Y, Cao, Y.C, Bi, Y.Z
Format:	Journal Article
Language:	English
Published:	Oxford Elsevier Ltd 10-03-2008 Elsevier Elsevier Limited
Subjects:	Allergy and Immunology Animals Animals, Newborn Applied microbiology Bacteria Bacteriophage T4 Bacteriophage T4 - immunology Biological and medical sciences Capsid - immunology Deoxyribonucleic acid DNA DNA, Viral - biosynthesis DNA, Viral - genetics DNA, Viral - immunology Electrophoresis, Polyacrylamide Gel Enzyme-Linked Immunosorbent Assay Escherichia coli - ultrastructure Escherichia coli - virology FMDV Foot & mouth disease Foot-and-Mouth Disease - immunology Foot-and-Mouth Disease - prevention & control Foot-and-Mouth Disease - virology Foot-and-mouth disease virus Foot-and-Mouth Disease Virus - genetics Foot-and-Mouth Disease Virus - immunology Foot-and-Mouth Disease Virus - pathogenicity Fundamental and applied biological sciences. Psychology Immunization Injection Injections, Subcutaneous Male Mice Mice, Inbred BALB C Microbiology Microscopy, Electron Miscellaneous Peptide Library Phage T4 platform vaccine Plasmids Potency challenge Promoter Regions, Genetic - genetics Protein Engineering Proteins Serotyping Swine T4 phage surface gene-protein display system Vaccines Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) Vaccines, Synthetic - therapeutic use Viral Vaccines - immunology Virology FMDV T4 phage surface gene-protein display system Phage T4 platform vaccine Bacteriophage T4 Potency challenge Picornaviridae Rodentia Vaccine Myoviridae Protein Virus Aphthovirus T even phage Vertebrata Phage display Mammalia Gene Mouse Veterinary Capsid Phage Phage T4
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Summary:	Summary An orally delivered foot-and-mouth disease (FMD) vaccine has not previously been reported. By using a T4 bacteriophage nanoparticle surface gene-protein display system (T4-S-GPDS), we created a foot-and-mouth disease virus (FMDV) entire capsid protein vaccine candidate. On the T4 phage surface SOC site, a full length FMDV capsid precursor polyprotein (P1, 755 aa) and proteinase 3C (213 aa) derived from an infected pig of serotype O strain GD-10 (1999), were separately displayed on different T4 phage particle surfaces through inserting their coding region DNAs into the T4 phage genome, yielding phage strains T4-P1 and T4-3C. We also constructed a series of FMDV sub-full length capsid structural protein (subunit) containing T4 phage recombinant vaccines. Both sucking and young BALB/c mice were used as two kinds of FMDV vaccine potency evaluation models. Many groups of both model mice were vaccinated orally or by subcutaneous injection with varying FMDV-T4 phage recombinant vaccines, with and without addition of adjuvant, then challenged with a lethal dose of cattle source virulent FMDV. In the case of immunization with a mixture of phage T4-P1 and phage T4-3C particles without any adjuvant added, all mice were 100% protected following either oral or injection immunization, whereas 100% of the control, non-immunized mice and mice immunized with only T4 phage vector Z1/Zh− or wild-type T4+ D phage died; in contrast, with FMDV subunit vaccine, less than 75% protection followed the same potency challenge in both mice model groups. In addition, two pigs immunized with a phage T4-P1 and phage T4-3C mix were protected upon housing together with infected pigs. This study represents a clear example of how FMD and other pathogenic disease vaccines can be prepared by a simple and efficient bacteriophage route.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0264-410X 1873-2518
DOI:	10.1016/j.vaccine.2007.12.053