Analysis of the vacA, cagA, cagE, iceA, and babA2 genes in Helicobacter pylori from sixty-one pediatric patients from the Midwestern United States

This study was designed to characterize H. pylori from pediatric gastric biopsy specimens in terms of several genes (vacA, cagA, cagE, iceA1, iceA2, and babA2) proposed to be involved in the pathogenesis of this organism. Many of these genes have been studied in adult H. pylori isolates, however, th...

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Bibliographic Details
Published in:	Diagnostic microbiology and infectious disease Vol. 46; no. 2; pp. 83 - 88
Main Authors:	Podzorski, Raymond P., Podzorski, Diane S., Wuerth, Ann, Tolia, Vasundhara
Format:	Journal Article
Language:	English
Published:	New York, NY Elsevier Inc 01-06-2003 Elsevier
Subjects:	Adhesins, Bacterial Antigens, Bacterial - genetics Bacterial Outer Membrane Proteins - genetics Bacterial Proteins - genetics Bacteriology Base Sequence Biological and medical sciences Biopsy, Needle Carrier Proteins - genetics Child Child, Preschool Cohort Studies DNA, Bacterial - analysis Female Fundamental and applied biological sciences. Psychology Genetic Predisposition to Disease Genetics Genotype Helicobacter Infections - diagnosis Helicobacter Infections - epidemiology Helicobacter Infections - genetics Helicobacter pylori Helicobacter pylori - genetics Helicobacter pylori - isolation & purification Helicobacter pylori - pathogenicity Humans Incidence Male Microbiology Midwestern United States - epidemiology Molecular Sequence Data Polymerase Chain Reaction - methods Risk Factors Sensitivity and Specificity Virulence United States North America America Midwestern United States USA Human Stomach Spirillales Digestive system Pathogenesis Spirillaceae Infection Helicobacter pylori Biopsy Bacteriosis Digestive diseases Bacteria Child
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Summary:	This study was designed to characterize H. pylori from pediatric gastric biopsy specimens in terms of several genes (vacA, cagA, cagE, iceA1, iceA2, and babA2) proposed to be involved in the pathogenesis of this organism. Many of these genes have been studied in adult H. pylori isolates, however, these genes have not been well characterized in H. pylori from children. Using PCR we observed that 44% of the H. pylori in our biopsies shared two common genotypes ( vacA s1b m1, cagA, cagE, iceA2 ± babA2). While 26% of the H. pylori had unique genotypes. The cag pathogenicity island associated genes, cagA and cagE, were found together in 64% or our H. pylori, while 84% were iceA2 positive. The presence of the babA2 gene has been proposed to be associated with a higher risk of H. pylori related diseases, however, we found that only 36% of our H. pylori contained this gene.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0732-8893 1879-0070
DOI:	10.1016/S0732-8893(03)00034-8