Search Results - "Wu, Zhengliang L"

Fluorescent glycan fingerprinting of SARS2 spike proteins by Wu, Zhengliang L., Ertelt, James M.

“…Glycosylation is the most common post-translational modification and has myriad of biological functions. However, glycan analysis has always been a challenge…”
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Glycoengineering of E‐Selectin Ligands by Intracellular versus Extracellular Fucosylation Differentially Affects Osteotropism of Human Mesenchymal Stem Cells by Dykstra, Brad, Lee, Jungmin, Mortensen, Luke J., Yu, Haixiao, Wu, Zhengliang L., Lin, Charles P., Rossi, Derrick J., Sackstein, Robert

“…Human mesenchymal stem cells (MSCs) hold great promise in cellular therapeutics for skeletal diseases but lack expression of E‐selectin ligands that direct…”
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Endoglycosidase assay using enzymatically synthesized fluorophore-labeled glycans as substrates to uncover enzyme substrate specificities by Wu, Zhengliang L., Ertelt, James M.

“…Glycan synthesis and degradation are not template but enzyme only driven processes. Substrate specificities of glyco-enzymes determine the structures of…”
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Imaging specific cellular glycan structures using glycosyltransferases via click chemistry by Wu, Zhengliang L, Person, Anthony D, Anderson, Matthew, Burroughs, Barbara, Tatge, Timothy, Khatri, Kshitij, Zou, Yonglong, Wang, Lianchun, Geders, Todd, Zaia, Joseph, Sackstein, Robert

“…Abstract Heparan sulfate (HS) is a polysaccharide fundamentally important for biologically activities. T/Tn antigens are universal carbohydrate cancer markers…”
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Phosphatase-coupled universal kinase assay and kinetics for first-order-rate coupling reaction by Wu, Zhengliang L

“…Kinases use adenosine-5'-triphosphate (ATP) as the donor substrate and generate adenosine-5'-diphosphate (ADP) as a product. An ADP-based phosphatase-coupled…”
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Inhibition of SULT4A1 expression induces up-regulation of phototransduction gene expression in 72-hour postfertilization zebrafish larvae by Crittenden, Frank, Thomas, Holly, Ethen, Cheryl M, Wu, Zhengliang L, Chen, Dongquan, Kraft, Timothy W, Parant, John M, Falany, Charles N

“…Sulfotransferase (SULT) 4A1 is an orphan enzyme that shares distinct structure and sequence similarities with other cytosolic SULTs. SULT4A1 is primarily…”
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Enzymatic synthesis of antithrombin III-binding heparan sulfate pentasaccharide by Rosenberg, Robert D, Kuberan, Balagurunathan, Lech, Miroslaw Z, Beeler, David L, Wu, Zhengliang L

“…Heparan sulfate (HS) proteoglycans are crucial to numerous biological processes and pathological conditions, but to date only a few HS structures have been…”
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The Involvement of Heparan Sulfate (HS) in FGF1/HS/FGFR1 Signaling Complex by Wu, Zhengliang L., Zhang, Lijuan, Yabe, Tomio, Kuberan, B., Beeler, David L., Love, Andre, Rosenberg, Robert D.

“…Fibroblast growth factor (FGF) signaling begins with the formation of a ternary complex of FGF, FGF receptor (FGFR), and heparan sulfate (HS). Multiple models…”
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Fluorescent Detection of O‑GlcNAc via Tandem Glycan Labeling by Wu, Zhengliang L, Luo, Ang, Grill, Alex, Lao, Taotao, Zou, Yonglong, Chen, Yue

“…O-GlcNAcylation is a reversible serine/threonine glycosylation on cytosolic and nuclear proteins that are involved in various regulatory pathways. However, the…”
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Assays for hyaluronidases and heparanase using nonreducing end fluorophore-labeled hyaluronan and heparan sulfate proteoglycan by Wu, Zhengliang L, Ertelt, James M

“…Abstract Glycosaminoglycans (GAGs), such as hyaluronan (HA) and heparan sulfate (HS), are a large group of polysaccharides found in the extracellular matrix…”
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Characterizing the Non-reducing End Structure of Heparan Sulfate by Wu, Zhengliang L., Lech, Miroslaw

“…The reducing end of heparan sulfate has been known for a long time, but information on the non-reducing end has been lacking. Recent studies indicate that the…”
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Detecting and Imaging O-GlcNAc Sites Using Glycosyltransferases: A Systematic Approach to Study O-GlcNAc by Wu, Zhengliang L, Tatge, Timothy J, Grill, Alex E, Zou, Yonglong

“…O-GlcNAcylation is a reversible serine/threonine glycosylation for regulating protein activity and availability inside cells. In a given protein,…”
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Detecting substrate glycans of fucosyltransferases with fluorophore-conjugated fucose and methods for glycan electrophoresis by Wu, Zhengliang L, Whittaker, Mark, Ertelt, James M, Person, Anthony D, Kalabokis, Vassili

“…Abstract Like sialylation, fucose usually locates at the nonreducing ends of various glycans on glycoproteins and constitutes important glycan epitopes…”
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Chemoenzymatic Synthesis of Classical and Non-classical Anticoagulant Heparan Sulfate Polysaccharides by Kuberan, Balagurunathan, Beeler, David L., Lech, Miroslaw, Wu, Zhengliang L., Rosenberg, Robert D.

“…Heparan sulfate (HS) polysaccharides interact with numerous proteins at the cell surface and orchestrate many different biological functions. Though many…”
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A versatile polyacrylamide gel electrophoresis based sulfotransferase assay by Wu, Zhengliang L, Ethen, Cheryl M, Larson, Sara, Prather, Brittany, Jiang, Weiping

“…Sulfotransferases are a large group of enzymes that regulate the biological activity or availability of a wide spectrum of substrates through sulfation with…”
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Modification degrees at specific sites on heparan sulphate: an approach to measure chemical modifications on biological molecules with stable isotope labelling by Wu, Zhengliang L, Lech, Miroslaw

“…Chemical modification of biological molecules is a general mechanism for cellular regulation. A quantitative approach has been developed to measure the extent…”
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A Liquid Chromatography-Mass Spectrometry-based Approach to Characterize the Substrate Specificity of Mammalian Heparanase by Mao, Yang, Huang, Yu, Buczek-Thomas, Jo Ann, Ethen, Cheryl M., Nugent, Matthew A., Wu, Zhengliang L., Zaia, Joseph

“…Extracellular heparanase activity releases growth factors and angiogenic factors from heparan sulfate (HS) storage sites and alters the integrity of the…”
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Direct fluorescent glycan labeling with recombinant sialyltransferases by Wu, Zhengliang L, Person, Anthony D, Burton, Andrew J, Singh, Ravinder, Burroughs, Barbara, Fryxell, Dan, Tatge, Timothy J, Manning, Timothy, Wu, Guoping, Swift, Karl A D, Kalabokis, Vassili

“…Abstract Glycosylation is a common modification found on numerous proteins and lipids. However, direct detection of glycans on these intact biomolecules has…”
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Non-reducing end labeling of heparan sulfate via click chemistry and a high throughput ELISA assay for heparanase by Wu, Zhengliang L, Huang, Xinyi, Ethen, Cheryl M, Tatge, Timothy, Pasek, Marta, Zaia, Joseph

“…Heparan sulfate (HS) is a linear polysaccharide found in the extracellular matrix (ECM) and on the cell membrane. It plays numerous roles in cellular events,…”
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Differential distribution of N- and O-Glycans and variable expression of sialyl-T antigen on HeLa cells—Revealed by direct fluorescent glycan imaging by Wu, Zhengliang L, Person, Anthony D, Zou, Yonglong, Burton, Andrew J, Singh, Ravinder, Burroughs, Barbara, Fryxell, Dan, Tatge, Timothy J, Manning, Timothy, Wu, Guoping, Swift, Karl A D, Kalabokis, Vassili

“…Abstract Cells are covered with glycans. The expression and distribution of specific glycans on the surface of a cell are important for various cellular…”
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