Search Results - "Wu, Wen Y."

Genome editing by natural and engineered CRISPR-associated nucleases by Wu, Wen Y., Lebbink, Joyce H. G., Kanaar, Roland, Geijsen, Niels, van der Oost, John

“…Over the last decade, research on distinct types of CRISPR systems has revealed many structural and functional variations. Recently, several novel types of…”
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Cryo-EM structure of the transposon-associated TnpB enzyme by Nakagawa, Ryoya, Hirano, Hisato, Omura, Satoshi N., Nety, Suchita, Kannan, Soumya, Altae-Tran, Han, Yao, Xiao, Sakaguchi, Yuriko, Ohira, Takayuki, Wu, Wen Y., Nakayama, Hiroshi, Shuto, Yutaro, Tanaka, Tatsuki, Sano, Fumiya K., Kusakizako, Tsukasa, Kise, Yoshiaki, Itoh, Yuzuru, Dohmae, Naoshi, van der Oost, John, Suzuki, Tsutomu, Zhang, Feng, Nureki, Osamu

“…The class 2 type V CRISPR effector Cas12 is thought to have evolved from the IS200/IS605 superfamily of transposon-associated TnpB proteins 1 . Recent studies…”
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The miniature CRISPR-Cas12m effector binds DNA to block transcription by Wu, Wen Y., Mohanraju, Prarthana, Liao, Chunyu, Adiego-Pérez, Belén, Creutzburg, Sjoerd C.A., Makarova, Kira S., Keessen, Karlijn, Lindeboom, Timon A., Khan, Tahseen S., Prinsen, Stijn, Joosten, Rob, Yan, Winston X., Migur, Anzhela, Laffeber, Charlie, Scott, David A., Lebbink, Joyce H.G., Koonin, Eugene V., Beisel, Chase L., van der Oost, John

“…CRISPR-Cas are prokaryotic adaptive immune systems. Cas nucleases generally use CRISPR-derived RNA guides to specifically bind and cleave DNA or RNA targets…”
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Live-cell imaging reveals the trade-off between target search flexibility and efficiency for Cas9 and Cas12a by Olivi, Lorenzo, Bagchus, Cleo, Pool, Victor, Bekkering, Ezra, Speckner, Konstantin, Offerhaus, Hidde, Wu, Wen Y, Depken, Martin, Martens, Koen J A, Staals, Raymond H J, Hohlbein, Johannes

“…CRISPR-Cas systems have widely been adopted as genome editing tools, with two frequently employed Cas nucleases being SpyCas9 and LbCas12a. Although both…”
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Host niche, genotype, and field location shape the diversity and composition of the soybean microbiome by YANG, Hong-jun, YE, Wen-wu, YU, Ze, SHEN, Wei-liang, LI, Su-zhen, WANG, Xing, CHEN, Jia-jia, WANG, Yuan-chao, ZHENG, Xiao-bo

“…Plant-associated microbes represent a key determinant of plant fitness through acquiring nutrients, promoting growth, and resisting to abiotic and biotic…”
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TldR: TnpB’s evolutionary shift from transposon nucleases to RNA-guided transcriptional regulators by Mohanraju, Prarthana, Wu, Wen Y.

“…TnpB proteins are transposon-encoded nucleases involved in transposon DNA propagation. Wiegand et al. identified a new class of TnpB-derived proteins, called…”
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A LAMP-assay-based specific microbiota analysis reveals community dynamics and potential interactions of 13 major soybean root pathogens by YE, Wen-wu, ZENG, Dan-dan, XU, Miao, YANG, Jin, MA, Jia-xin, WANG, Yuan-chao, ZHENG, Xiao-bo

“…Soybean root diseases are associated with numerous fungal and oomycete pathogens; however, the community dynamics and interactions of these pathogens are…”
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Multiplex gene editing by CRISPR–Cpf1 using a single crRNA array by Zetsche, Bernd, Heidenreich, Matthias, Mohanraju, Prarthana, Fedorova, Iana, Kneppers, Jeroen, DeGennaro, Ellen M, Winblad, Nerges, Choudhury, Sourav R, Abudayyeh, Omar O, Gootenberg, Jonathan S, Wu, Wen Y, Scott, David A, Severinov, Konstantin, van der Oost, John, Zhang, Feng

“…Multiplexed genome editing is simplified by harnessing the ability of Cpf1 to process its own pre-crRNA. Targeting of multiple genomic loci with Cas9 is…”
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Good guide, bad guide: spacer sequence-dependent cleavage efficiency of Cas12a by Creutzburg, Sjoerd C A, Wu, Wen Y, Mohanraju, Prarthana, Swartjes, Thomas, Alkan, Ferhat, Gorodkin, Jan, Staals, Raymond H J, van der Oost, John

“…Abstract Genome editing has recently made a revolutionary development with the introduction of the CRISPR–Cas technology. The programmable CRISPR-associated…”
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Mechanistic and evolutionary insights into a type V-M CRISPR–Cas effector enzyme by Omura, Satoshi N., Nakagawa, Ryoya, Südfeld, Christian, Villegas Warren, Ricardo, Wu, Wen Y., Hirano, Hisato, Laffeber, Charlie, Kusakizako, Tsukasa, Kise, Yoshiaki, Lebbink, Joyce H. G., Itoh, Yuzuru, van der Oost, John, Nureki, Osamu

“…RNA-guided type V CRISPR–Cas12 effectors provide adaptive immunity against mobile genetic elements (MGEs) in bacteria and archaea. Among diverse Cas12 enzymes,…”
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Cryo-EM structure of the transposonassociated TnpB enzyme by Nakagawa, Ryoya, Hirano, Hisato, Omura, Satoshi N, Nety, Suchita, Kannan, Soumya, Altae-Tran, Han, Yao, Xiao, Sakaguchi, Yuriko, Ohira, Takayuki, Wu, Wen Y, Nakayama, Hiroshi, Shuto, Yutaro, Tanaka, Tatsuki, Sano, Fumiya K, Kusakizako, Tsukasa, Kise, Yoshiaki, Itoh, Yuzuru, Dohmae, Naoshi, van der Oost, John, Suzuki, Tsutomu, Zhang, Feng, Nureki, Osamu

“…The class 2 type V CRISPR effector Cas12 is thought to have evolved from the 1S200/ 1S605 superfamily of transposon-associated TnpB proteins1. Recent studies…”
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Functional and Structural Outcomes Following Lensectomy for Ectopia Lentis by Wu-Chen, Wen Y., Letson, Robert D., Summers, C. Gail

“…Purpose: High refractive errors and optical aberrations reduce vision when the lens edge bisects the pupil. We studied outcomes of eyes with ectopia lentis…”
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Erratum: Multiplex gene editing by CRISPR–Cpf1 using a single crRNA array by Zetsche, Bernd, Heidenreich, Matthias, Mohanraju, Prarthana, Fedorova, Iana, Kneppers, Jeroen, DeGennaro, Ellen M, Winblad, Nerges, Choudhury, Sourav R, Abudayyeh, Omar O, Gootenberg, Jonathan S, Wu, Wen Y, Scott, David A, Severinov, Konstantin, van der Oost, John, Zhang, Feng

“…Nat. Biotechnol. 35, 31–34 (2017); published online 5 December 2016; corrected after print 12 January 2017 In the version of this article initially published,…”
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Multiplex gene editing by CRISPR-Cpf1 through autonomous processing of a single crRNA array by Zetsche, Bernd, Heidenreich, Matthias, Mohanraju, Prarthana, Fedorova, Iana, Kneppers, Jeroen, DeGennaro, Ellen M., Winblad, Nerges, Choudhury, Sourav R., Abudayyeh, Omar O., Gootenberg, Jonathan S., Wu, Wen Y., Scott, David A., Severinov, Konstantin, van der Oost, John, Zhang, Feng

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Ophthalmic manifestations of Wolf–Hirschhorn syndrome by Wu-Chen, Wen Y., Christiansen, Stephen P., Berry, Susan A., Engel, W.Keith, Fray, Katherine J., Summers, C.Gail

“…Wolf–Hirschhorn syndrome is caused by partial deletion of the short arm of chromosome 4 (4p−). Common features include developmental delay, microcephaly,…”
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Folliculitis Decalvans Developing 20 Years After Hair Restoration Surgery in Punch Grafts: Case Report by OTBERG, NINA, WU, WEN‐YU, KANG, HOON, MARTINKA, MAGDALENA, ALZOLIBANI, ABDULLATEEF A., RESTREPO, ISABEL, SHAPIRO, JERRY

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Uniportal versus biportal video-assisted thoracoscopic sympathectomy for palmar hyperhidrosis by Chen, Yong-bing, Ye, Wu, Yang, Wen-tao, Shi, Li, Guo, Xu-feng, Xu, Zhong-hua, Qian, Yong-yue

“…Background Video-assisted thoracoscopic sympathectomy had replaced open surgery. The aim of this study was to compare the outcomes of using a single port and…”
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Successful Treatment of Temporal Triangular Alopecia by Hair Restoration Surgery Using Follicular Unit Transplantation by WU, WEN‐YU, OTBERG, NINA, KANG, HOON, ZANET, LUCIANNA, SHAPIRO, JERRY

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