RNAi-mediated Ghrelin affects gastric H+ –K+ -ATPase activity and expression of GOAT–Ghrelin system in vitro

Highlights • shGhrelin inhibited ghrelin mRNA expression via an RNAi mechanism. • shGhrelin reduced total and acylated ghrelin production and secretion ( P < 0.05). • GOAT mRNA expression was down-regulated ( P < 0.05) in shGhrelin group. • Ghrelin gene silencing inhibited the activity of H+ –...

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Published in:	General and comparative endocrinology Vol. 228; pp. 48 - 52
Main Authors:	Du, Gai M, Wu, Jie G, Luo, Bi P, Hu, Zhi H, Li, Liu A, Liu, Mao J
Format:	Journal Article
Language:	English
Published:	United States Elsevier Inc 01-03-2016
Subjects:	Acylated ghrelin Acyltransferases - genetics Acyltransferases - metabolism Animals Cells, Cultured Endocrinology & Metabolism Gastric Mucosa - metabolism Gene Expression Regulation Ghrelin - antagonists & inhibitors Ghrelin - genetics Ghrelin - metabolism GOAT H(+)-K(+)-Exchanging ATPase - genetics H(+)-K(+)-Exchanging ATPase - metabolism H+–K+-ATPase activity In Vitro Techniques Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction RNA Interference RNA, Messenger - genetics RNA, Small Interfering - genetics shGhrelin Swine shGhrelin H +–K +-ATPase activity GOAT Acylated ghrelin H+–K+-ATPase activity H(+)–K(+)-ATPase activity
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Summary:	Highlights • shGhrelin inhibited ghrelin mRNA expression via an RNAi mechanism. • shGhrelin reduced total and acylated ghrelin production and secretion ( P < 0.05). • GOAT mRNA expression was down-regulated ( P < 0.05) in shGhrelin group. • Ghrelin gene silencing inhibited the activity of H+ –K+ -ATPase and pepsin ( P < 0.05).
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0016-6480 1095-6840
DOI:	10.1016/j.ygcen.2016.02.009