Search Results - "Wrzesinski, Krzysztof"

Determination of drug toxicity using 3D spheroids constructed from an immortal human hepatocyte cell line by Fey, Stephen J, Wrzesinski, Krzysztof

“…Numerous publications have documented that the immortal cells grown in three-dimensional (3D) cultures possess physiological behavior, which is more…”
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Anticancer Potential of Sutherlandia frutescens and Xysmalobium undulatum in LS180 Colorectal Cancer Mini-Tumors by Gouws, Chrisna, Smit, Tanya, Willers, Clarissa, Svitina, Hanna, Calitz, Carlemi, Wrzesinski, Krzysztof

“…Colorectal cancer remains to be one of the leading causes of death worldwide, with millions of patients diagnosed each year. Although chemotherapeutic drugs…”
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From 2D to 3D--a New Dimension for Modelling the Effect of Natural Products on Human Tissue by Wrzesinski, Krzysztof, Fey, Stephen J

“…Natural products, or their synthetic derivatives are a treasure trove to find potential candidates for novel drugs for human treatment. The selection of…”
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A Novel NCI-H69AR Drug-Resistant Small-Cell Lung Cancer Mini-Tumor Model for Anti-Cancer Treatment Screening by van Niekerk, Alandi, Wrzesinski, Krzysztof, Steyn, Dewald, Gouws, Chrisna

“…Small-cell lung cancer is a fast-growing carcinoma with a poor prognosis and a high level of relapse due to multi-drug resistance (MDR). Genetic mutations that…”
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Metabolic Reprogramming and the Recovery of Physiological Functionality in 3D Cultures in Micro-Bioreactors by Wrzesinski, Krzysztof, Fey, Stephen J

“…The recovery of physiological functionality, which is commonly seen in tissue mimetic three-dimensional (3D) cellular aggregates (organoids, spheroids, acini,…”
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The cultural divide: exponential growth in classical 2D and metabolic equilibrium in 3D environments by Wrzesinski, Krzysztof, Rogowska-Wrzesinska, Adelina, Kanlaya, Rattiyaporn, Borkowski, Kamil, Schwämmle, Veit, Dai, Jie, Joensen, Kira Eyd, Wojdyla, Katarzyna, Carvalho, Vasco Botelho, Fey, Stephen J

“…Cellular metabolism can be considered to have two extremes: one is characterized by exponential growth (in 2D cultures) and the other by a dynamic equilibrium…”
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Hepatocellular carcinoma (HepG2/C3A) cell-based 3D model for genotoxicity testing of chemicals by Štampar, Martina, Sedighi Frandsen, Helle, Rogowska-Wrzesinska, Adelina, Wrzesinski, Krzysztof, Filipič, Metka, Žegura, Bojana

“…The major weakness of the current in vitro genotoxicity test systems is the inability of the indicator cells to express metabolic enzymes needed for the…”
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A novel NCI‐H69V small cell lung cancer functional mini‐tumor model for future treatment screening applications by Merwe, Liezaan, Svitina, Hanna, Willers, Clarissa, Wrzesinski, Krzysztof, Gouws, Chrisna

“…Small cell lung cancer (SCLC) is aggressive and despite multiple clinical trials, its standard of care is unchanged for the past three decades. In vitro cancer…”
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In vitro evaluation of the anti-melanoma effects (A375 cell line) of the gel and whole leaf extracts from selected aloe species by Laux, Alex, Hamman, Josias, Svitina, Hanna, Wrzesinski, Krzysztof, Gouws, Chrisna

“…Melanoma is one of the most common occurring malignancies in humans. The melanoma response rate to the standard anticancer drug, dacarbazine, is relatively…”
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Phosphoproteome Analysis of Functional Mitochondria Isolated from Resting Human Muscle Reveals Extensive Phosphorylation of Inner Membrane Protein Complexes and Enzymes by Zhao, Xiaolu, León, Ileana R., Bak, Steffen, Mogensen, Martin, Wrzesinski, Krzysztof, Højlund, Kurt, Jensen, Ole Nørregaard

“…Mitochondria play a central role in energy metabolism and cellular survival, and consequently mitochondrial dysfunction is associated with a number of human…”
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Top-down and Middle-down Protein Analysis Reveals that Intact and Clipped Human Histones Differ in Post-translational Modification Patterns[S] by Tvardovskiy, Andrey, Wrzesinski, Krzysztof, Sidoli, Simone, Fey, StephenJ, Rogowska-Wrzesinska, Adelina, Jensen, OleN

“…Post-translational modifications (PTMs) of histone proteins play a fundamental role in regulation of DNA-templated processes. There is also growing evidence…”
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Response to and recovery from treatment in human liver-mimetic clinostat spheroids: a model for assessing repeated-dose drug toxicity by Fey, Stephen J, Korzeniowska, Barbara, Wrzesinski, Krzysztof

“…Abstract Medicines are usually prescribed for repeated use over shorter or longer times. Unfortunately, repeated-dose animal toxicity studies do not correlate…”
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Microgravity spheroids as a reliable, long-term tool for predictive toxicology by Fey, Stephen J., Wrzesinski, Krzysztof

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Characterization of an Alginate Encapsulated LS180 Spheroid Model for Anti-colorectal Cancer Compound Screening by Smit, Tanya, Calitz, Carlemi, Willers, Clarissa, Svitina, Hanna, Hamman, Josias, Fey, Stephen J, Gouws, Chrisna, Wrzesinski, Krzysztof

“…Colorectal cancer is one of the leading causes of cancer-related deaths. A main problem for its treatment is resistance to chemotherapy, requiring the…”
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Clinostat 3D Cell Culture: Protocols for the Preparation and Functional Analysis of Highly Reproducible, Large, Uniform Spheroids and Organoids by Wrzesinski, Krzysztof, Frandsen, Helle Sedighi, Calitz, Carlemi, Gouws, Chrisna, Korzeniowska, Barbara, Fey, Stephen J

“…Growing cells as 3D structures need not be difficult. Often, it is not necessary to change cell type, additives or growth media used. All that needs to be…”
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A sub-chronic Xysmalobium undulatum hepatotoxicity investigation in HepG2/C3A spheroid cultures compared to an in vivo model by Calitz, Carlemi, Hamman, Josias H., Fey, Stephen J., Viljoen, Alvaro M., Gouws, Chrisna, Wrzesinski, Krzysztof

“…Traditional herbal medicines are utilized by 27 million South Africans. Xysmalobium undulatum (Uzara) is one of the most widely used traditional medicinal…”
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Cell-free DNA in a three-dimensional spheroid cell culture model: A preliminary study by Aucamp, Janine, Calitz, Carlemi, Bronkhorst, Abel J., Wrzesinski, Krzysztof, Hamman, Sias, Gouws, Chrisna, Pretorius, Piet J.

“…Investigating the biological functions of cell-free DNA (cfDNA) is limited by the interference of vast numbers of putative sources and causes of DNA release…”
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The role of reversible cysteine oxidations in intracellular signaling – mass spectrometry based study of acetaminophen driven redox imbalance by Rogowska-Wrzesinska, Adelina, Wrzesinski, Krzysztof, Wojdyla, Katarzyna, Williamson, James, Fey, Steven J.

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P-49 - The role of reversible cysteine oxidations in intracellular signaling – mass spectrometry based study of acetaminophen driven redox imbalance by Rogowska-Wrzesinska, Adelina, Wrzesinski, Krzysztof, Wojdyla, Katarzyna, Williamson, James, Fey, Steven J.

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Recent advances in three-dimensional cell culturing to assess liver function and dysfunction: from a drug biotransformation and toxicity perspective by Calitz, Carlemi, Hamman, Josias H, Fey, Stephen J, Wrzesinski, Krzysztof, Gouws, Chrisna

“…The liver is a vital organ fulfilling a central role in over 500 major metabolic functions, including serving as the most essential site for drug…”
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