Search Results - "Wrenbeck, Emily E"

Single-mutation fitness landscapes for an enzyme on multiple substrates reveal specificity is globally encoded by Wrenbeck, Emily E., Azouz, Laura R., Whitehead, Timothy A.

“…Our lack of total understanding of the intricacies of how enzymes behave has constrained our ability to robustly engineer substrate specificity. Furthermore,…”
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Trade-offs between enzyme fitness and solubility illuminated by deep mutational scanning by Klesmith, Justin R., Bacik, John-Paul, Wrenbeck, Emily E., Michalczyk, Ryszard, Whitehead, Timothy A.

“…Proteins are marginally stable, and an understanding of the sequence determinants for improved protein solubility is highly desired. For enzymes, it is well…”
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Deep sequencing methods for protein engineering and design by Wrenbeck, Emily E, Faber, Matthew S, Whitehead, Timothy A

“…•Deep sequencing permits examination of local protein fitness landscapes.•NGS aids in engineering affinity and specificity for protein molecular…”
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Impact of In Vivo Protein Folding Probability on Local Fitness Landscapes by Faber, Matthew S, Wrenbeck, Emily E, Azouz, Laura R, Steiner, Paul J, Whitehead, Timothy A

“…Abstract It is incompletely understood how biophysical properties like protein stability impact molecular evolution and epistasis. Epistasis is defined as…”
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Plasmid-based one-pot saturation mutagenesis by Wrenbeck, Emily E, Klesmith, Justin R, Stapleton, James A, Adeniran, Adebola, Tyo, Keith E J, Whitehead, Timothy A

“…Nicking mutagenesis is a streamlined protocol for plasmid-based single-pot comprehensive saturation mutagenesis. Deep mutational scanning is a foundational…”
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An Automated Data-Driven Pipeline for Improving Heterologous Enzyme Expression by Wrenbeck, Emily E, Bedewitz, Matthew A, Klesmith, Justin R, Noshin, Syeda, Barry, Cornelius S, Whitehead, Timothy A

“…Enzymes are the ultimate entities responsible for chemical transformations in natural and engineered biosynthetic pathways. However, many natural enzymes…”
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