Search Results - "Wood, Jay F"

Conformational stability, folding, and ligand-binding affinity of single-chain Fv immunoglobulin fragments expressed in Escherichia coli by Pantoliano, Michael W, Bird, Robert E, Johnson, Syd, Asel, Eric D, Dodd, Steven W, Wood, Jay F, Hardman, Karl D

“…A fluorescein-binding single-chain Fv (scFv) was chosen as a model for the study of the physicochemical parameters associated with synthetic IgG fragments…”
Get full text

Large increases in general stability for subtilisin BPN' through incremental changes in the free energy of unfolding by Pantoliano, Michael W, Whitlow, Marc, Wood, Jay F, Dodd, Steven W, Hardman, Karl D, Rollence, Michele L, Bryan, Philip N

“…Six individual amino acid substitutions at separate positions in the tertiary structure of subtilisin BPN' (EC 3.4.21.14) were found to increase the stability…”
Get full text

The engineering of binding affinity at metal ion binding sites for the stabilization of proteins: subtilisin as a test case by Pantoliano, Michael W, Whitlow, Marc, Wood, Jay F, Rollence, Michele L, Finzel, Barry C, Gilliland, Gary L, Poulos, Thomas L, Bryan, Philip N

“…A weak Ca2+ binding site in the bacterial serine protease subtilisin BPN' (EC 3.4.21.14) was chosen as a model to explore the feasibility of stabilizing a…”
Get full text

Protein engineering of subtilisin BPN': enhanced stabilization through the introduction of two cysteines to form a disulfide bond by Pantoliano, Michael W, Ladner, Robert C, Bryan, Philip N, Rollence, Michele L, Wood, Jay F, Poulos, Thomas L

“…Introduction of a disulfide bond by site-directed mutagenesis was found to enhance the stability of subtilisin BPN' (EC 3.4.21.14) under a variety of…”
Get full text