Enzyme-linked immunosorbent assay based on monoclonal and polyclonal antibodies for the detection of Entamoeba histolytica antigens in faecal specimens

A murine monoclonal antibody (MAb) (Eh208C2-2) raised against crude lysate of the pathogenic HM-1:IMSS strain of Entamoeba histolytica was used in an enzyme-linked immunosorbent assay for the detection of E. histolytica antigens in faecal specimens. The detection limit of the assay was 110 and 280 a...

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Bibliographic Details
Published in:	Transactions of the Royal Society of Tropical Medicine and Hygiene Vol. 86; no. 2; p. 166
Main Authors:	Wonsit, R, Thammapalerd, N, Tharavanij, S, Radomyos, P, Bunnag, D
Format:	Journal Article
Language:	English
Published:	England 01-03-1992
Subjects:	Animals Antibodies, Protozoan - analysis Antigens, Protozoan - analysis Entamoeba histolytica - immunology Entamoeba histolytica - isolation & purification Entamoebiasis - diagnosis Enzyme-Linked Immunosorbent Assay Feces - parasitology Humans
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Summary:	A murine monoclonal antibody (MAb) (Eh208C2-2) raised against crude lysate of the pathogenic HM-1:IMSS strain of Entamoeba histolytica was used in an enzyme-linked immunosorbent assay for the detection of E. histolytica antigens in faecal specimens. The detection limit of the assay was 110 and 280 amoebae/ml of the HM-1:IMSS and HK-9 strains in phosphate-buffered saline, respectively. The assay was applied to single stool samples from 3 groups of individuals comprising 40 patients whose stools were positive for E. histolytica trophozoites and/or cysts (group I), 48 patients whose stools were negative for E. histolytica but positive for other parasites (group II), and 36 parasitologically-negative healthy controls (group III). Positivity rates of 77.5%, 2.1% and 2.7% were found in samples from groups I, II and III respectively. Specificity, positive and negative predictive values, and efficiency of the assay were 97.6%, 93.9%, 90.1% and 91.1% respectively. When group I samples were further divided into a trophozoite-positive subgroup IA (13 samples) and a cyst-positive subgroup IB (27 samples), the positive rates were 100% and 66.7%, respectively (P < 0.025).
ISSN:	0035-9203
DOI:	10.1016/0035-9203(92)90553-O