Sustained Release of Epidermal Growth Factor Accelerates Wound Repair

Epidermal growth factor (EGF) is a potent mitogen in vitro, but its biological role is less clear. The vulnerary effects of EGF were evaluated in a model of wound repair, the polyvinyl alcohol sponge implanted subcutaneously in rats. EGF was purified to homogeneity by reverse-phase HPLC and quantifi...

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Published in:	Proceedings of the National Academy of Sciences - PNAS Vol. 82; no. 21; pp. 7340 - 7344
Main Authors:	Buckley, Anne, Davidson, Jeffrey M., Kamerath, Craig D., Wolt, Terrill B., Woodward, Stephen C.
Format:	Journal Article
Language:	English
Published:	Washington, DC National Academy of Sciences of the United States of America 01-11-1985 National Acad Sciences
Subjects:	Amino acids Animals Biological and medical sciences Cell growth Cell physiology Collagen - biosynthesis Collagens Controlled release DNA DNA Replication - drug effects Drug Implants Epidermal Growth Factor - administration & dosage Epidermal Growth Factor - isolation & purification Epidermal Growth Factor - pharmacology Fibroblasts Fundamental and applied biological sciences. Psychology Granulation tissue Granulation Tissue - drug effects Granulation Tissue - pathology Male Mice Molecular and cellular biology Placebos Rats Rats, Inbred Strains Receptors Responses to growth factors, tumor promotors, other factors Sponges Wound Healing - drug effects Vertebrata Mammalia Epidermal growth factor Rat Animal Rodentia Models Acceleration Mechanism of action Repair Wound Biological activity
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Summary:	Epidermal growth factor (EGF) is a potent mitogen in vitro, but its biological role is less clear. The vulnerary effects of EGF were evaluated in a model of wound repair, the polyvinyl alcohol sponge implanted subcutaneously in rats. EGF was purified to homogeneity by reverse-phase HPLC and quantified by receptor binding assay and amino acid analysis. Preliminary data showed moderate promotion of granulation tissue formation by daily injections of 10 μ g of EGF. To test the hypothesis that long-term exposure to EGF is required for complete cellular response, the factor was incorporated into pellets releasing 10 or 20 μ g of biologically active EGF per day, and the pellets were embedded within the sponges. Slow release of EGF caused a dramatic increase in the extent and organization of the granulation tissue at day 7, a doubling in the DNA content, and 33% increases in protein content and wet weight, as compared with placebo controls. Although collagen content was also increased by almost 50%, the relative rate of collagen synthesis remained the same, suggesting that the morphological and biochemical increase in collagen resulted from increased numbers of fibroblasts rather than a specific stimulation of collagen synthesis. These results indicate that the local sustained presence of EGF accelerates the process of wound repair, specifically neovascularization, organization by fibroblasts, and accumulation of collagen.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0027-8424 1091-6490
DOI:	10.1073/pnas.82.21.7340