Search Results - "Wohlschlager, Therese"

Native mass spectrometry combined with enzymatic dissection unravels glycoform heterogeneity of biopharmaceuticals by Wohlschlager, Therese, Scheffler, Kai, Forstenlehner, Ines C., Skala, Wolfgang, Senn, Stefan, Damoc, Eugen, Holzmann, Johann, Huber, Christian G.

“…Robust manufacturing processes resulting in consistent glycosylation are critical for the efficacy and safety of biopharmaceuticals. Information on…”
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Exploring sample preparation and data evaluation strategies for enhanced identification of host cell proteins in drug products of therapeutic antibodies and Fc-fusion proteins by Esser-Skala, Wolfgang, Segl, Marius, Wohlschlager, Therese, Reisinger, Veronika, Holzmann, Johann, Huber, Christian G.

“…Manufacturing of biopharmaceuticals involves recombinant protein expression in host cells followed by extensive purification of the target protein. Yet, host…”
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Towards middle-up analysis of polyclonal antibodies: subclass-specific N-glycosylation profiling of murine immunoglobulin G (IgG) by means of HPLC-MS by Blöchl, Constantin, Regl, Christof, Huber, Christian G., Winter, Petra, Weiss, Richard, Wohlschlager, Therese

“…In recent years, advanced HPLC-MS strategies based on intact protein (“top-down”) or protein subunit (“middle-up/middle-down”) analysis have been implemented…”
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Methylated glycans as conserved targets of animal and fungal innate defense by Wohlschlager, Therese, Butschi, Alex, Grassi, Paola, Sutov, Grigorij, Gauss, Robert, Hauck, Dirk, Schmieder, Stefanie S, Knobel, Martin, Titz, Alexander, Dell, Anne, Haslam, Stuart M, Hengartner, Michael O, Aebi, Markus, Künzler, Markus

“…Effector proteins of innate immune systems recognize specific non-self epitopes. Tectonins are a family of β-propeller lectins conserved from bacteria to…”
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“Small is beautiful” – Examining reliable determination of low-abundant therapeutic antibody glycovariants by Böttinger, Katharina, Regl, Christof, Schäpertöns, Veronika, Rapp, Erdmann, Wohlschlager, Therese, Huber, Christian G.

“…Glycans associated with biopharmaceutical drugs play crucial roles in drug safety and efficacy, and therefore, their reliable detection and quantification is…”
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Membrane cholesterol and sphingomyelin, and ostreolysin A are obligatory for pore-formation by a MACPF/CDC-like pore-forming protein, pleurotolysin B by Ota, Katja, Leonardi, Adrijana, Mikelj, Miha, Skočaj, Matej, Wohlschlager, Therese, Künzler, Markus, Aebi, Markus, Narat, Mojca, Križaj, Igor, Anderluh, Gregor, Sepčić, Kristina, Maček, Peter

“…The mushroom Pleurotus ostreatus has been reported to produce the hemolytic proteins ostreolysin (OlyA), pleurotolysin A (PlyA) and pleurotolysin B (PlyB). The…”
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How many human proteoforms are there? by Aebersold, Ruedi, Agar, Jeffrey N, Amster, I Jonathan, Baker, Mark S, Bertozzi, Carolyn R, Boja, Emily S, Costello, Catherine E, Cravatt, Benjamin F, Fenselau, Catherine, Garcia, Benjamin A, Ge, Ying, Gunawardena, Jeremy, Hendrickson, Ronald C, Hergenrother, Paul J, Huber, Christian G, Ivanov, Alexander R, Jensen, Ole N, Jewett, Michael C, Kelleher, Neil L, Kiessling, Laura L, Krogan, Nevan J, Larsen, Martin R, Loo, Joseph A, Ogorzalek Loo, Rachel R, Lundberg, Emma, MacCoss, Michael J, Mallick, Parag, Mootha, Vamsi K, Mrksich, Milan, Muir, Tom W, Patrie, Steven M, Pesavento, James J, Pitteri, Sharon J, Rodriguez, Henry, Saghatelian, Alan, Sandoval, Wendy, Schlüter, Hartmut, Sechi, Salvatore, Slavoff, Sarah A, Smith, Lloyd M, Snyder, Michael P, Thomas, Paul M, Uhlén, Mathias, Van Eyk, Jennifer E, Vidal, Marc, Walt, David R, White, Forest M, Williams, Evan R, Wohlschlager, Therese, Wysocki, Vicki H, Yates, Nathan A, Young, Nicolas L, Zhang, Bing

“…Despite decades of accumulated knowledge about proteins and their post-translational modifications (PTMs), numerous questions remain regarding their molecular…”
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Expression, Purification, and Functional Characterization of Tectonin 2 from Laccaria bicolor: A Six-Bladed Beta-Propeller Lectin Specific for O-Methylated Glycans by Wohlschlager, Therese, Titz, Alexander, Künzler, Markus, Varrot, Annabelle

“…Tectonins are conserved defense proteins of innate immune systems featuring a β-propeller fold. Tectonin 2 from Laccaria bicolor, Lb-Tec2, is the first fungal…”
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A Generic HPLC Method for Absolute Quantification of Oxidation in Monoclonal Antibodies and Fc-Fusion Proteins Using UV and MS Detection by Regl, Christof, Wohlschlager, Therese, Holzmann, Johann, Huber, Christian G

“…Oxidation of biopharmaceuticals may affect their bioactivity, serum half-life, and (bio)­chemical stability. The Fc domain of IgG monoclonal antibodies (mAbs)…”
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Glycoproteomics of a Single Protein: Revealing Tens of Thousands of Myozyme Glycoforms by Hybrid HPLC-MS Approaches by Di Marco, Fiammetta, Blöchl, Constantin, Esser-Skala, Wolfgang, Schäpertöns, Veronika, Zhang, Tao, Wuhrer, Manfred, Sandra, Koen, Wohlschlager, Therese, Huber, Christian G.

“…Characterization of highly glycosylated biopharma-ceuticals by mass spectrometry is challenging because of the huge chemical space of coexistent glycoforms…”
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MoFi: A Software Tool for Annotating Glycoprotein Mass Spectra by Integrating Hybrid Data from the Intact Protein and Glycopeptide Level by Skala, Wolfgang, Wohlschlager, Therese, Senn, Stefan, Huber, Gabriel E, Huber, Christian G

“…Hybrid mass spectrometry (MS) is an emerging technique for characterizing glycoproteins, which typically display pronounced microheterogeneity. Since hybrid MS…”
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A Simple Strategy to Eliminate Hexosylation Bias in the Relative Quantification of N‐Glycosylation in Biopharmaceuticals by Esser‐Skala, Wolfgang, Wohlschlager, Therese, Regl, Christof, Huber, Christian G.

“…N‐glycosylation may affect the safety and efficacy of biopharmaceuticals and is thus monitored during manufacturing. Mass spectrometry of the intact protein is…”
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Exploring the Chemical Space of Protein Glycosylation in Noncovalent Protein Complexes: An Expedition along Different Structural Levels of Human Chorionic Gonadotropin by Employing Mass Spectrometry by Lebede, Maximilian, Di Marco, Fiammetta, Esser-Skala, Wolfgang, Hennig, René, Wohlschlager, Therese, Huber, Christian G

“…Modern analytical approaches employing high-resolution mass spectrometry (MS) facilitate the generation of a vast amount of structural data of highly complex…”
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Crystal Structures of Fungal Tectonin in Complex with O-Methylated Glycans Suggest Key Role in Innate Immune Defense by Sommer, Roman, Makshakova, Olga N., Wohlschlager, Therese, Hutin, Stephanie, Marsh, May, Titz, Alexander, Künzler, Markus, Varrot, Annabelle

“…Innate immunity is the first line of defense against pathogens and predators. To initiate a response, it relies on the detection of invaders, where…”
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Dilute-and-shoot analysis of therapeutic monoclonal antibody variants in fermentation broth: a method capability study by Regl, Christof, Wohlschlager, Therese, Esser-Skala, Wolfgang, Wagner, Iris, Samonig, Martin, Holzmann, Johann, Huber, Christian G

“…Monoclonal antibodies (mAbs) are widely applied as highly specific and efficient therapeutic agents for various medical conditions, including cancer,…”
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Eine einfache Strategie zur Korrektur des Fehlers aufgrund von Hexosylierung bei relativer Quantifizierung der N‐Glykosylierungsvarianten von Biopharmazeutika by Esser‐Skala, Wolfgang, Wohlschlager, Therese, Regl, Christof, Huber, Christian G.

“…N‐Glykane können sich auf die Sicherheit und Wirksamkeit von Biopharmazeutika auswirken, weswegen sie bei deren Herstellung kontrolliert werden. Zur…”
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Structure–function relationship of a novel fucoside-binding fruiting body lectin from Coprinopsis cinerea exhibiting nematotoxic activity by Bleuler-Martinez, Silvia, Varrot, Annabelle, Olieric, Vincent, Schubert, Mario, Vogt, Eva, Fetz, Céline, Wohlschlager, Therese, Plaza, David Fernando, Wälti, Martin, Duport, Yannick, Capitani, Guido, Aebi, Markus, Künzler, Markus

“…Abstract Lectins are non-immunoglobulin-type proteins that bind to specific carbohydrate epitopes and play important roles in intra- and inter-organismic…”
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Nematotoxicity of Marasmius oreades Agglutinin (MOA) Depends on Glycolipid Binding and Cysteine Protease Activity by Wohlschlager, Therese, Butschi, Alex, Zurfluh, Katrin, Vonesch, Sibylle C., auf dem Keller, Ulrich, Gehrig, Peter, Bleuler-Martinez, Silvia, Hengartner, Michael O., Aebi, Markus, Künzler, Markus

“…Fruiting body lectins have been proposed to act as effector proteins in the defense of fungi against parasites and predators. The Marasmius oreades agglutinin…”
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Burkavidin: A novel secreted biotin-binding protein from the human pathogen Burkholderia pseudomallei by Sardo, Alessia, Wohlschlager, Therese, Lo, Cheikh, Zoller, Heinz, Ward, Thomas R., Creus, Marc

“…The avidin–biotin technology has many applications, including molecular detection; immobilization; protein purification; construction of supramolecular…”
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Inhibition of Aspergillus fumigatus conidia binding to extracellular matrix proteins by sialic acids: a pH effect? by Tiralongo, Joe, Wohlschlager, Therese, Tiralongo, Evelin, Kiefel, Milton J

“…1 Institute for Glycomics, Gold Coast Campus, Griffith University, QLD 4222, Australia 2 School of Pharmacy, Gold Coast Campus, Griffith University, QLD 4222,…”
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