Search Results - "Wittwer, Carl"

High-resolution DNA melting analysis: advancements and limitations by Wittwer, Carl T

“…Recent advances in fluorescent dyes, methods, instruments and software for DNA melting analysis have created versatile new tools for variant scanning and…”
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Rapid Cycle and Extreme Polymerase Chain Reaction by Wittwer, Carl T

“…Rapid cycle polymerase chain reaction (PCR) amplifies DNA in 10-30 min, while extreme PCR is complete in less than 1 min. These methods do not sacrifice…”
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Extreme PCR: efficient and specific DNA amplification in 15-60 seconds by Farrar, Jared S, Wittwer, Carl T

“…PCR is a key technology in molecular biology and diagnostics that typically amplifies and quantifies specific DNA fragments in about an hour. However, the…”
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uMELT: prediction of high-resolution melting curves and dynamic melting profiles of PCR products in a rich web application by DWIGHT, Zachary, PALAIS, Robert, WITTWER, Carl T

“…uMelt(SM) is a flexible web-based tool for predicting DNA melting curves and denaturation profiles of PCR products. The user defines an amplicon sequence and…”
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MIQE: A Step Toward More Robust and Reproducible Quantitative PCR by Bustin, Stephen A, Wittwer, Carl T

“…A detailed examination of the qPCR evidence revealed poor assay design, widespread disregard of control results suggesting contamination issues, inadequate…”
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The Digital MIQE Guidelines Update: Minimum Information for Publication of Quantitative Digital PCR Experiments for 2020 by Huggett, Jim F

“…Abstract Digital PCR (dPCR) has developed considerably since the publication of the Minimum Information for Publication of Digital PCR Experiments (dMIQE)…”
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The digital MIQE guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments by Huggett, Jim F, Foy, Carole A, Benes, Vladimir, Emslie, Kerry, Garson, Jeremy A, Haynes, Ross, Hellemans, Jan, Kubista, Mikael, Mueller, Reinhold D, Nolan, Tania, Pfaffl, Michael W, Shipley, Gregory L, Vandesompele, Jo, Wittwer, Carl T, Bustin, Stephen A

“…There is growing interest in digital PCR (dPCR) because technological progress makes it a practical and increasingly affordable technology. dPCR allows the…”
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High resolution melting analysis for gene scanning by Erali, Maria, Wittwer, Carl T.

“…High resolution melting is a new method of genotyping and variant scanning that can be seamlessly appended to PCR amplification. Limitations of genotyping by…”
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The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments by Bustin, Stephen A, Benes, Vladimir, Garson, Jeremy A, Hellemans, Jan, Huggett, Jim, Kubista, Mikael, Mueller, Reinhold, Nolan, Tania, Pfaffl, Michael W, Shipley, Gregory L, Vandesompele, Jo, Wittwer, Carl T

“…Currently, a lack of consensus exists on how best to perform and interpret quantitative real-time PCR (qPCR) experiments. The problem is exacerbated by a lack…”
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The Effect of Single Mismatches on Primer Extension by Rejali, Nick A, Moric, Endi, Wittwer, Carl T

“…Allele-specific PCR is an important diagnostic tool that identifies single-nucleotide variants by preferential amplification of a particular allele, using…”
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Democratizing the Real-Time PCR by Wittwer, Carl T

“…In 1993, we submitted an NIH technology transfer research grant to add flow cytometry optics to rapid-cycle PCR, using an obsolete flow cytometer from our…”
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High-resolution DNA melting analysis for simple and efficient molecular diagnostics by Reed, Gudrun H, Kent, Jana O, Wittwer, Carl T

“…High-resolution melting of DNA is a simple solution for genotyping, mutation scanning and sequence matching. The melting profile of a PCR product depends on…”
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Portable Nanopore Sequencing for Viral Surveillance by Wittwer, Carl T

“…The bioinformatics were complicated enough that the data required uploading by the Internet to the Cloud and downloading for central processing in the UK…”
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DNA melting analysis by Wittwer, Carl T., Hemmert, Andrew C., Kent, Jana O., Rejali, Nick A.

“…Melting is a fundamental property of DNA that can be monitored by absorbance or fluorescence. PCR conveniently produces enough DNA to be directly monitored on…”
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Simultaneous mutation scanning and genotyping by high-resolution DNA melting analysis by Wittwer, Carl T, Montgomery, Jesse, Palais, Robert, Zhou, Luming

“…This protocol permits the simultaneous mutation scanning and genotyping of PCR products by high-resolution DNA melting analysis. This is achieved using…”
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Sensitivity and Specificity of Single-Nucleotide Polymorphism Scanning by High-Resolution Melting Analysis by Reed, Gudrun H, Wittwer, Carl T

“…Screening for heterozygous sequence changes in PCR products, also known as "mutation scanning", is an important tool for genetic research and clinical…”
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Tietz Textbook of Laboratory Medicine by Rifai, Nader

“…Use THE definitive reference for laboratory medicine and clinical pathology! Tietz Textbook of Laboratory Medicine, 7th Edition provides the guidance necessary…”
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High resolution melting applications for clinical laboratory medicine by Erali, Maria, Voelkerding, Karl V., Wittwer, Carl T.

“…Separation of the two strands of DNA with heat (melting) is a fundamental property of DNA that is conveniently monitored with fluorescence. Conventional…”
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Nearest-neighbour transition-state analysis for nucleic acid kinetics by Rejali, Nick A, Ye, Felix D, Zuiter, Aisha M, Keller, Caroline C, Wittwer, Carl T

“…Abstract We used stopped-flow to monitor hypochromicity for 43 oligonucleotide duplexes to study nucleic acid kinetics and extract transition-state parameters…”
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A "Culture" Shift: Broad Bacterial Detection, Identification, and Antimicrobial Susceptibility Testing Directly from Whole Blood by Andini, Nadya, Hu, Anne, Zhou, Luming, Cogill, Steven, Wang, Tza-Huei, Wittwer, Carl T, Yang, Samuel

“…The time required for bloodstream pathogen detection, identification (ID), and antimicrobial susceptibility testing (AST) does not satisfy the acute needs of…”
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