Search Results - "Wittwer, C T"

The LightCycler: a microvolume multisample fluorimeter with rapid temperature control by Wittwer, C T, Ririe, K M, Andrew, R V, David, D A, Gundry, R A, Balis, U J

“…Experimental and commercial microvolume fluorimeters with rapid temperature control are described. Fluorescence optics adopted from flow cytometry were used to…”
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Quantification of low-copy transcripts by continuous SYBR Green I monitoring during amplification by Morrison, T B, Weis, J J, Wittwer, C T

“…Continuous fluorescence observation of amplifying DNA allows rapid and accurate quantification of initial transcript copy number. A simple and generic method…”
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Continuous fluorescence monitoring of rapid cycle DNA amplification by Wittwer, C T, Herrmann, M G, Moss, A A, Rasmussen, R P

“…Rapid cycle DNA amplification was continuously monitored by three different fluorescence techniques. Fluorescence was monitored by (i) the…”
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Real-time fluorescence genotyping of factor V Leiden during rapid-cycle PCR by Lay, Marla J, Wittwer, Carl T

“…A single-step method for factor V Leiden genotyping is presented that uses rapid-cycle PCR and simultaneous fluorescence analysis with resonance energy…”
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Quantifying variant differences in DNA melting curves: Effects of length, melting rate, and curve overlay by Li, M., Palais, R.A., Zhou, L., Wittwer, C.T.

“…High resolution DNA melting of PCR products is a simple technique for sequence variant detection and analysis. However, sensitivity and specificity vary and…”
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Homogeneous Multiplex Genotyping of Hemochromatosis Mutations with Fluorescent Hybridization Probes by Bernard, Philip S., Ajioka, Richard S., Kushner, James P., Wittwer, Carl T.

“…Multiplex polymerase chain reaction amplification and genotyping by fluorescent probe melting temperature ( T m) was used to simultaneously detect multiple…”
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Rapid cycle DNA amplification: time and temperature optimization by Wittwer, C T, Garling, D J

“…Rapid temperature cycling with hot air allows rigorous optimization of the times and temperatures required for each stage of the polymerase chain reaction. A…”
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Homogeneous amplification and variant detection by fluorescent hybridization probes by Bernard, P S, Wittwer, C T

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Quantification of HER2/neu Gene Amplification by Competitive PCR Using Fluorescent Melting Curve Analysis by Lyon, Elaine, Millson, Alison, Lowery, Mary C, Woods, Rachel, Wittwer, Carl T

“…Molecular detection methods for HER2/neu gene amplification include fluorescence in situ hybridization (FISH) and competitive PCR. We designed a quantitative…”
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Detection of chromosomal translocations in formalin-fixed paraffin-embedded (FFPE) leukemic specimens by digital expression profiling by Liew, M., Mao, R., Wittwer, C. T., Salama, M. E.

“…Summary Introduction Detection of chromosomal translocations in formalin‐fixed paraffin‐embedded (FFPE) leukemic samples is important for confirmation of…”
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Oligonucleotide melting temperatures under PCR conditions: nearest-neighbor corrections for Mg(2+), deoxynucleotide triphosphate, and dimethyl sulfoxide concentrations with comparison to alternative empirical formulas by von Ahsen, N, Wittwer, C T, Schütz, E

“…Many techniques in molecular biology depend on the oligonucleotide melting temperature (T(m)), and several formulas have been developed to estimate T(m)…”
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Fully Automated DNA Reaction and Analysis in a Fluidic Capillary Instrument by Swerdlow, Harold, Jones, Barbara J, Wittwer, Carl T

“…A simple, reliable, automated genetic analysis instrument has been designed and prototyped. The system uses novel fluidic technology, coupling thermal cycling,…”
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Fluorescein-Labeled Oligonucleotides for Real-Time PCR: Using the Inherent Quenching of Deoxyguanosine Nucleotides by Crockett, Andrew O., Wittwer, Carl T.

“…Fluorescein-labeled oligonucleotide probes can be used to continuously monitor the polymerase chain reaction. Depending on the sequence, the fluorescence…”
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Automated polymerase chain reaction in capillary tubes with hot air by WITTWER, C. T, FILLMORE, G. C, HILLYARD, D. R

“…We describe a simple, compact, inexpensive thermal cycler that can be used for the polymerase chain reaction. Based on heat transfer with air to samples in…”
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Product Differentiation by Analysis of DNA Melting Curves during the Polymerase Chain Reaction by Ririe, Kirk M., Rasmussen, Randy P., Wittwer, Carl T.

“…A microvolume fluorometer integrated with a thermal cycler was used to acquire DNA melting curves during polymerase chain reaction by fluorescence monitoring…”
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Real-Time Multiplex PCR Assays by Wittwer, Carl T., Herrmann, Mark G., Gundry, Cameron N., Elenitoba-Johnson, Kojo S.J.

“…The ability to multiplex PCR by probe color and melting temperature (Tm) greatly expands the power of real-time analysis. Simple hybridization probes with only…”
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Identification of monoclonal B-cell populations by rapid cycle polymerase chain reaction. A practical screening method for the detection of immunoglobulin gene rearrangements by Segal, GH, Wittwer, CT, Fishleder, AJ, Stoler, MH, Tubbs, RR, Kjeldsberg, CR

“…Alternatives to Southern blot hybridization for gene rearrangement analysis are being studied because of the time, labor, cost, and radioisotopes required for…”
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Antigen expression and polymerase chain reaction amplification of mantle cell lymphomas by MOLOT, R. J, MEEKER, T. C, WITTWER, C. T, PERKINS, S. L, SEGAL, G. H, MASIH, A. S, BRAYLAN, R. C, KJELDSBERG, C. R

“…Flow immunophenotyping, DNA content analysis, and polymerase chain reaction (PCR) amplification for t(11;14) and t(14;18) were performed on 11 cases of typical…”
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Syngeneic Bone Marrow Transplantation Without Conditioning in a Patient With Paroxysmal Nocturnal Hemoglobinuria: In Vivo Evidence That the Mutant Stem Cells Have a Survival Advantage by Endo, Morito, Beatty, Patrick G., Vreeke, Teresa M., Wittwer, Carl T., Singh, Shashi P., Parker, Charles J.

“…A 10-year-old girl with paroxysmal nocturnal hemoglobinuria (PNH) received an infusion of syngeneic bone marrow without preparative marrow ablation or…”
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Minimizing the time required for DNA amplification by efficient heat transfer to small samples by Wittwer, C T, Fillmore, G C, Garling, D J

“…Hot-air temperature cycling of 1- to 10-microliters samples in glass capillary tubes can amplify DNA by the polymerase chain reaction in 15 min or less. A…”
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