Synthesis and Characterization of Ceramide-Containing Liposomes as Membrane Models for Different T Cell Subpopulations

A fine balance of regulatory (Treg) and conventional CD4+ T cells (Tconv) is required to prevent harmful immune responses, while at the same time ensuring the development of protective immunity against pathogens. As for many cellular processes, sphingolipid metabolism also crucially modulates the Tr...

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Bibliographic Details
Published in:	Journal of functional biomaterials Vol. 13; no. 3; p. 111
Main Authors:	Eder, Sascha, Hollmann, Claudia, Mandasari, Putri, Wittmann, Pia, Schumacher, Fabian, Kleuser, Burkhard, Fink, Julian, Seibel, Jürgen, Schneider-Schaulies, Jürgen, Stigloher, Christian, Beyersdorf, Niklas, Dembski, Sofia
Format:	Journal Article
Language:	English
Published:	Basel MDPI AG 02-08-2022 MDPI
Subjects:	CD4 antigen Cell culture cell membrane model Cell membranes Ceramide Chromatography Flow cytometry Hand tools Investigations Lipid metabolism Lipids liposome Liposomes Lymphocytes Lymphocytes T Membranes Splenocytes Subpopulations T cell receptors Virology United States > US Germany
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Summary:	A fine balance of regulatory (Treg) and conventional CD4+ T cells (Tconv) is required to prevent harmful immune responses, while at the same time ensuring the development of protective immunity against pathogens. As for many cellular processes, sphingolipid metabolism also crucially modulates the Treg/Tconv balance. However, our understanding of how sphingolipid metabolism is involved in T cell biology is still evolving and a better characterization of the tools at hand is required to advance the field. Therefore, we established a reductionist liposomal membrane model system to imitate the plasma membrane of mouse Treg and Tconv with regards to their ceramide content. We found that the capacity of membranes to incorporate externally added azide-functionalized ceramide positively correlated with the ceramide content of the liposomes. Moreover, we studied the impact of the different liposomal preparations on primary mouse splenocytes in vitro. The addition of liposomes to resting, but not activated, splenocytes maintained viability with liposomes containing high amounts of C16-ceramide being most efficient. Our data thus suggest that differences in ceramide post-incorporation into Treg and Tconv reflect differences in the ceramide content of cellular membranes.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 These authors share the senior author position due to their similarly high conceptional input into this study and the writing of the manuscript. These authors contributed equally to this work.
ISSN:	2079-4983 2079-4983
DOI:	10.3390/jfb13030111