Search Results - "Withers, S.G"

Glycosyltransferases: structures, functions, and mechanisms by Lairson, L L, Henrissat, B, Davies, G J, Withers, S G

“…Glycosyltransferases catalyze glycosidic bond formation using sugar donors containing a nucleoside phosphate or a lipid phosphate leaving group. Only two…”
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(369) - A-Antigen Re-Expression and Prevention of Hyperacute Rejection Following In Vivo Enzymatic Treatment in a Model of ABO-Incompatible Transplantation by Erickson, T., Motyka, B., Tao, K., Pearcey, J., Rahfeld, P., Kizhakkedathu, J.N., Cypel, M., Cowan, P.J., Withers, S.G., West, L.J.

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Stereoselective hydrolysis catalyzed by related beta-1,4-glucanases and beta-1,4-xylanases by J Gebler, N R Gilkes, M Claeyssens, D B Wilson, P Béguin, W W Wakarchuk, D G Kilburn, R C Miller, Jr, R A Warren, S G Withers

“…Over 80 beta-1,4-glucanases and beta-1,4-xylanases can be classified into one of eight families on the basis of amino acid sequence similarities in their…”
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Glu280 is the nucleophile in the active site of Clostridium thermocellum CelC, a family A endo-beta-1,4-glucanase by Wang, Q, Tull, D, Meinke, A, Gilkes, N R, Warren, R A, Aebersold, R, Withers, S G

“…A new mechanism-based inactivator of beta-1,4-glucanases, 2',4'-dinitrophenyl-2-deoxy-2-fluoro-beta-D-cellobioside, was synthesized and used to trap the…”
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(731) Enzymatic Removal of A-Antigen in a Mouse Model of ABO-Incompatible (ABOi) Transplantation by Erickson, T., Motyka, B., Xu, L., Tao, K., Pearcey, J., Cypel, M., Kizhakkedathu, J.N., Rahfeld, P., Cowan, P.J., Withers, S.G., West, L.J.

“…The ABO histo-blood group system presents a major barrier in organ transplantation that limits organ availability. A reduction in donor A/B antigens may…”
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Mechanism-Based Covalent Neuraminidase Inhibitors with Broad-Spectrum Influenza Antiviral Activity by Kim, Jin-Hyo, Resende, Ricardo, Wennekes, Tom, Chen, Hong-Ming, Bance, Nicole, Buchini, Sabrina, Watts, Andrew G., Pilling, Pat, Streltsov, Victor A., Petric, Martin, Liggins, Richard, Barrett, Susan, McKimm-Breschkin, Jennifer L., Niikura, Masahiro, Withers, Stephen G.

“…Influenza antiviral agents play important roles in modulating disease severity and in controlling pandemics while vaccines are prepared, but the development of…”
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Suicide inactivation of human prostatic acid phosphatase and a phosphotyrosine phosphatase by Wang, Q, Dechert, U, Jirik, F, Withers, S G

“…4-Difluoromethylphenyl bis(cyclohexylammonium) phosphate was synthesized in 4 steps starting from dibenzyl phosphite and shown to be a time-dependent suicide…”
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Glutamic acid 274 is the nucleophile in the active site of a "retaining" exoglucanase from Cellulomonas fimi by TULL, D, WITHERS, S. G, GILKES, N. R, KILBURN, D. G, ANTONY, R, WARREN, R. A. J, AEBERSOLD, R

“…In addition to its known substrate activity with p-nitrophenyl beta-cellobioside, the exoglucanase from Cellulomonas fimi has been shown to utilize substituted…”
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Region-directed mutagenesis of residues surrounding the active site nucleophile in beta-glucosidase from Agrobacterium faecalis by Trimbur, D.E. (University of British Columbia, Vancouver, BC, Canada), Warren, R.A.J, Withers, S.G

“…The active site nucleophile of the beta-glucosidase of Agrobacterium faecalis has recently been identified by the use of inhibitors. A combination of…”
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Mechanisms of glycosyl transferases and hydrolases by Withers, S.G

“…Glycosidases and glycosyl transferases fall into two major mechanistic classes; those that hydrolyse the glycosidic bond with retention of anomeric…”
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Mechanistic Analysis of the Blood Group Antigen-Cleaving endo-β-Galactosidase from Clostridium perfringens by Shaikh, Fathima Aidha, Randriantsoa, Mialy, Withers, Stephen G

“…The A and B antigens are of vital importance in blood transfusion and organ transplantation. The specificity of EABase, an endo-β-galactosidase from C…”
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Identification of the Active Site Nucleophile in Jack Bean α-Mannosidase Using 5-Fluoro-β-l-Gulosyl Fluoride by Howard, Steven, He, Shouming, Withers, Stephen G.

“…Mannosidases play a key role in the processing of glycoproteins and thus are of considerable pharmaceutical interest and indeed have emerged as targets for the…”
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Cloning, Expression, Characterization, and Nucleophile Identification of Family 3, Aspergillus nigerβ-Glucosidase by Dan, Siegel, Marton, Ira, Dekel, Mara, Bravdo, Ben-Ami, He, Shouming, Withers, Stephen G., Shoseyov, Oded

“…The β-glucosidase from Aspergillus niger (CMI CC 324262) was purified, and an N-terminal sequence and two internal sequences were determined. BglI genomic gene…”
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Unequivocal identification of Asp-214 as the catalytic nucleophile of Saccharomyces cerevisiae alpha-glucosidase using 5-fluoro glycosyl fluorides by McCarter, J D, Withers, S G

“…Yeast alpha-glucosidase is a member of a sequence-related family of alpha-glycosidases (Family 13) that includes important digestive alpha-amylases and…”
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Exploring the Cellulose/Xylan Specificity of the β-1,4-Glycanase Cex from Cellulomonas fimi through Crystallography and Mutation by Notenboom, Valerie, Birsan, Camelia, Warren, R. Antony J, Withers, Stephen G, Rose, David R

“…The retaining β-1,4-glycanase Cex from Cellulomonas fimi, a family 10 glycosyl hydrolase, hydrolyzes xylan 40-fold more efficiently than cellulose. To gain…”
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Crystal structure of the catalytic domain of the .beta.-1,4-glycanase Cex from Cellulomonas fimi by White, Andre, Withers, Stephen G, Gilkes, Neil R, Rose, David R

“…beta-1,4-Glycanases, principally cellulases and xylanases, are responsible for the hydrolysis of plant biomass. The bifunctional beta-1,4-xylanase/glucanase…”
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Mechanisms of Cellulases and Xylanases: A Detailed Kinetic Study of the Exo-.beta.-1,4-glycanase from Cellulomonas Fimi by Tull, Dedreia, Withers, Stephen G

“…The exoglucanase/xylanase from Cellulomonas fimi (Cex) has been subjected to a detailed kinetic investigation with a range of aryl beta-D-glycoside substrates…”
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Identification of Glutamic Acid 78 as the Active Site Nucleophile in Bacillus subtilis Xylanase Using Electrospray Tandem Mass Spectrometry by Miao, Shichang, Ziser, Lothar, Aebersold, Ruedi, Withers, Stephen G

“…A new mechanism-based inactivator of beta-1, 4-xylanases, 2',4'-dinitrophenyl 2-deoxy-2-fluoro-beta-xylobioside, has been synthesized and used to trap the…”
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Assignment of Sweet Almond β-Glucosidase as a Family 1 Glycosidase and Identification of Its Active Site Nucleophile by He, Shouming, Withers, Stephen G.

“…Sweet almond β-glucosidase is a well studied glycosidase, having been subjected to numerous kinetic analyses and inhibition studies. However, it is not known…”
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The Acid/Base Catalyst in the Exoglucanase/Xylanase from Cellulomonas fimi Is Glutamic Acid 127: Evidence from Detailed Kinetic Studies of Mutants by MacLeod, Alasdair M, Lindhorst, Thisbe, Withers, Stephen G, Warren, R. Antony J

“…The exoglucanase/xylanase Cex from Cellulomonas fimi hydrolyzes beta-1,4-glycosidic bonds with net retention of anomeric configuration, releasing the…”
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