Search Results - "Wiseman, Paul W."

Improved genetically encoded near-infrared fluorescent calcium ion indicators for in vivo imaging by Qian, Yong, Cosio, Danielle M Orozco, Piatkevich, Kiryl D, Aufmkolk, Sarah, Su, Wan-Chi, Celiker, Orhan T, Schohl, Anne, Murdock, Mitchell H, Aggarwal, Abhi, Chang, Yu-Fen, Wiseman, Paul W, Ruthazer, Edward S, Boyden, Edward S, Campbell, Robert E

“…Near-infrared (NIR) genetically encoded calcium ion (Ca2+) indicators (GECIs) can provide advantages over visible wavelength fluorescent GECIs in terms of…”
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CaMKII Triggers the Diffusional Trapping of Surface AMPARs through Phosphorylation of Stargazin by Opazo, Patricio, Labrecque, Simon, Tigaret, Cezar M., Frouin, Arnaud, Wiseman, Paul W., De Koninck, Paul, Choquet, Daniel

“…The Ca 2+/calmodulin-dependent protein kinase II (CaMKII) is critically required for the synaptic recruitment of AMPA-type glutamate receptors (AMPARs) during…”
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Cholesterol Modulates CFTR Confinement in the Plasma Membrane of Primary Epithelial Cells by Abu-Arish, Asmahan, Pandzic, Elvis, Goepp, Julie, Matthes, Elizabeth, Hanrahan, John W., Wiseman, Paul W.

“…The cystic fibrosis transmembrane conductance regulator (CFTR) is a plasma-membrane anion channel that, when mutated, causes the disease cystic fibrosis…”
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Spatiotemporal Image Correlation Spectroscopy (STICS) Theory, Verification, and Application to Protein Velocity Mapping in Living CHO Cells by Hebert, Benedict, Costantino, Santiago, Wiseman, Paul W.

“…We introduce a new extension of image correlation spectroscopy (ICS) and image cross-correlation spectroscopy (ICCS) that relies on complete analysis of both…”
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Modular actin nano-architecture enables podosome protrusion and mechanosensing by van den Dries, Koen, Nahidiazar, Leila, Slotman, Johan A., Meddens, Marjolein B. M., Pandzic, Elvis, Joosten, Ben, Ansems, Marleen, Schouwstra, Joost, Meijer, Anke, Steen, Raymond, Wijers, Mietske, Fransen, Jack, Houtsmuller, Adriaan B., Wiseman, Paul W., Jalink, Kees, Cambi, Alessandra

“…Basement membrane transmigration during embryonal development, tissue homeostasis and tumor invasion relies on invadosomes, a collective term for invadopodia…”
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Chemo-mechanical diffusion waves explain collective dynamics of immune cell podosomes by Gong, Ze, van den Dries, Koen, Migueles-Ramírez, Rodrigo A., Wiseman, Paul W., Cambi, Alessandra, Shenoy, Vivek B.

“…Immune cells, such as macrophages and dendritic cells, can utilize podosomes, mechanosensitive actin-rich protrusions, to generate forces, migrate, and patrol…”
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Advances in image correlation spectroscopy: measuring number densities, aggregation states, and dynamics of fluorescently labeled macromolecules in cells by Kolin, David L, Wiseman, Paul W

“…A brief historical outline of fluorescence fluctuation correlation techniques is presented, followed by an in-depth review of the theory and development of…”
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STICCS Reveals Matrix-Dependent Adhesion Slipping and Gripping in Migrating Cells by Toplak, Tim, Pandzic, Elvis, Chen, Lingfeng, Vicente-Manzanares, Miguel, Horwitz, Alan Rick, Wiseman, Paul W.

“…Two-color spatio-temporal image cross-correlation spectroscopy (STICCS) is a new, to our knowledge, image analysis method that calculates space-time…”
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Nonspecific binding of common anti-CFTR antibodies in ciliated cells of human airway epithelium by Sato, Yukiko, Mustafina, Kamila R., Luo, Yishan, Martini, Carolina, Thomas, David Y., Wiseman, Paul W., Hanrahan, John W.

“…There is evidence that the cystic fibrosis transmembrane conductance regulator (CFTR) anion channel is highly expressed at the apical pole of ciliated cells in…”
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Actomyosin-dependent dynamic spatial patterns of cytoskeletal components drive mesoscale podosome organization by Meddens, Marjolein B. M., Pandzic, Elvis, Slotman, Johan A., Guillet, Dominique, Joosten, Ben, Mennens, Svenja, Paardekooper, Laurent M., Houtsmuller, Adriaan B., van den Dries, Koen, Wiseman, Paul W., Cambi, Alessandra

“…Podosomes are cytoskeletal structures crucial for cell protrusion and matrix remodelling in osteoclasts, activated endothelial cells, macrophages and dendritic…”
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Magnitude and Direction of Vesicle Dynamics in Growing Pollen Tubes Using Spatiotemporal Image Correlation Spectroscopy and Fluorescence Recovery after Photobleaching by Bove, Jérôme, Vaillancourt, Benoit, Kroeger, Jens, Hepler, Peter K, Wiseman, Paul W, Geitmann, Anja

“…The delivery of cell wall material and membrane to growing plant cell surfaces requires the spatial and temporal coordination of secretory vesicle trafficking…”
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The integrin-ligand interaction regulates adhesion and migration through a molecular clutch by Chen, Lingfeng, Vicente-Manzanares, Miguel, Potvin-Trottier, Laurent, Wiseman, Paul W, Horwitz, Alan Rick

“…Adhesive and migratory behavior can be cell type, integrin, and substrate dependent. We have compared integrin and substrate differences using three integrin…”
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Stoichiometry of molecular complexes at adhesions in living cells by Digman, Michelle A, Wiseman, Paul W, Choi, Colin, Horwitz, Alan R, Gratton, Enrico

“…We describe a method to detect molecular complexes and measure their stoichiometry in living cells from simultaneous fluctuations of the fluorescence intensity…”
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Molecular Flow Quantified beyond the Diffraction Limit by Spatiotemporal Image Correlation of Structured Illumination Microscopy Data by Ashdown, George W., Cope, Andrew, Wiseman, Paul W., Owen, Dylan M.

“…We combine total internal reflection fluorescence structured illumination microscopy with spatiotemporal image correlation spectroscopy to quantify the flow…”
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Activity-Dependent Netrin-1 Secretion Drives Synaptic Insertion of GluA1-Containing AMPA Receptors in the Hippocampus by Glasgow, Stephen D., Labrecque, Simon, Beamish, Ian V., Aufmkolk, Sarah, Gibon, Julien, Han, Dong, Harris, Stephanie N., Dufresne, Paul, Wiseman, Paul W., McKinney, R. Anne, Séguéla, Philippe, De Koninck, Paul, Ruthazer, Edward S., Kennedy, Timothy E.

“…Dynamic trafficking of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid glutamate receptors (AMPARs) to synapses is critical for activity-dependent…”
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Actin-myosin network reorganization breaks symmetry at the cell rear to spontaneously initiate polarized cell motility by Yam, Patricia T, Wilson, Cyrus A, Ji, Lin, Hebert, Benedict, Barnhart, Erin L, Dye, Natalie A, Wiseman, Paul W, Danuser, Gaudenz, Theriot, Julie A

“…We have analyzed the spontaneous symmetry breaking and initiation of actin-based motility in keratocytes (fish epithelial cells). In stationary keratocytes,…”
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Spatial Intensity Distribution Analysis Reveals Abnormal Oligomerization of Proteins in Single Cells by Godin, Antoine G., Rappaz, Benjamin, Potvin-Trottier, Laurent, Kennedy, Timothy E., De Koninck, Yves, Wiseman, Paul W.

“…Knowledge of membrane receptor organization is essential for understanding the initial steps in cell signaling and trafficking mechanisms, but quantitative…”
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Revealing protein oligomerization and densities in situ using spatial intensity distribution analysis by Godin, Antoine G., Costantino, Santiago, Lorenzo, Louis-Etienne, Swift, Jody L., Sergeev, Mikhail, Ribeiro-da-Silva, Alfredo, De Koninck, Yves, Wiseman, Paul W., Snyder, Solomon H.

“…Measuring protein interactions is key to understanding cell signaling mechanisms, but quantitative analysis of these interactions in situ has remained a major…”
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Fluctuation Imaging Spiced Up with a Piece of PIE by Wiseman, Paul W.

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Detecting Protein Complexes in Living Cells from Laser Scanning Confocal Image Sequences by the Cross Correlation Raster Image Spectroscopy Method by Digman, Michelle A., Wiseman, Paul W., Horwitz, Alan R., Gratton, Enrico

“…We describe a general method for detecting molecular complexes based on the analysis of single molecule fluorescence fluctuations from laser scanning confocal…”
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