Search Results - "Wintermeyer, W."

FIDELITY OF AMINOACYL-tRNA SELECTION ON THE RIBOSOME: Kinetic and Structural Mechanisms by Rodnina, Marina V, Wintermeyer, Wolfgang

“…The ribosome discriminates between correct and incorrect aminoacyl-tRNAs (aa-tRNAs), or their complexes with elongation factor Tu (EF-Tu) and GTP, according to…”
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Mechanisms of elongation on the ribosome: dynamics of a macromolecular machine by Wintermeyer, W, Peske, F, Beringer, M, Gromadski, K B, Savelsbergh, A, Rodnina, M V

“…Protein synthesis in the cell is performed on ribosomes, large ribonucleoprotein particles, which in bacteria consist of three RNA molecules and over 50…”
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GTP hydrolysis by EF-G synchronizes tRNA movement on small and large ribosomal subunits by Holtkamp, Wolf, Cunha, Carlos E, Peske, Frank, Konevega, Andrey L, Wintermeyer, Wolfgang, Rodnina, Marina V

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GTP Consumption of Elongation Factor Tu During Translation of Heteropolymeric mRNAs by Rodnina, Marina V., Wintermeyer, Wolfgang

“…The stoichiometry of elongation factor Tu (EF-Tu) and GTP in the complex with aminoacyl-tRNA and the consumption of GTP during peptide bond formation on the…”
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Ribosomal RNA is the target for oxazolidinones, a novel class of translational inhibitors by MATASSOVA, N.B., RODNINA, M.V., ENDERMANN, R., KROLL, H.-P., PLEISS, U., WILD, H., WINTERMEYER, W.

“…Oxazolidinones are antibacterial agents that act primarily against gram-positive bacteria by inhibiting protein synthesis. The binding of oxazolidinones to 70S…”
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Codon‐dependent conformational change of elongation factor Tu preceding GTP hydrolysis on the ribosome by Rodnina, M.V., Fricke, R., Kuhn, L., Wintermeyer, W.

“…The mechanisms by which elongation factor Tu (EF‐Tu) promotes the binding of aminoacyl‐tRNA to the A site of the ribosome and, in particular, how GTP…”
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The ``Allosteric Three-Site Model" of Elongation Cannot Be Confirmed in a Well-Defined Ribosome System from Escherichia coli by Semenkov, Yuri P., Rodnina, Marina V., Wintermeyer, Wolfgang

“…For the functional role of the ribosomal tRNA exit (E) site, two different models have been proposed. It has been suggested that transient E-site binding of…”
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Elongation factor G-induced structural change in helix 34 of 16S rRNA related to translocation on the ribosome by MATASSOVA, NATALIA B., RODNINA, MARINA V., WINTERMEYER, WOLFGANG

“…During the translocation step of the elongation cycle, two tRNAs together with the mRNA move synchronously and rapidly on the ribosome. The movement is…”
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Binding of the 3′ terminus of tRNA to 23S rRNA in the ribosomal exit site actively promotes translocation by Lill, R., Robertson, J.M., Wintermeyer, W.

“…A key event in ribosomal protein synthesis is the translocation of deacylated tRNA, peptidyl tRNA and mRNA, which is catalyzed by elongation factor G (EF‐G)…”
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The G222D mutation in elongation factor Tu inhibits the codon‐induced conformational changes leading to GTPase activation on the ribosome by Vorstenbosch, E., Pape, T., Rodnina, M. V., Kraal, B., Wintermeyer, W.

“…Elongation factor Tu (EF‐Tu) from Escherichia coli carrying the mutation G222D is unable to hydrolyze GTP on the ribosome and to sustain polypeptide synthesis…”
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Truncated Elongation Factor G Lacking the G Domain Promotes Translocation of the 3′End but not of the Anticodon Domain of Peptidyl-tRNA by Borowski, Christian, Rodnina, Marina V., Wintermeyer, Wolfgang

“…The mechanism by which elongation factor G (EF-G) catalyzes the translocation of tRNAs and mRNA on the ribosome is not known. The reaction requires GTP, which…”
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Conformational changes in the bacterial SRP receptor FtsY upon binding of guanine nucleotides and SRP by Jagath, Junutula R, Rodnina, Marina V, Wintermeyer, Wolfgang

“…In cotranslational preprotein targeting in Escherichia coli, the signal recognition particle (SRP) binds to the signal peptide emerging from the ribosome and,…”
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Mechanism of elongation factor G function in tRNA translocation on the ribosome by Wintermeyer, W, Savelsbergh, A, Semenkov, Y P, Katunin, V I, Rodnina, M V

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Purification of fMet-tRNA(fMet) by fast protein liquid chromatography by Rodnina, M V, Semenkov, Y P, Wintermeyer, W

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Form Follows Function: Structure of an Elongation Factor G-Ribosome Complex by Rodnina, Marina V., Wintermeyer, Wolfgang

“…The structure of an elongation factor G-ribosome complex is analyzed for functional information…”
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How ribosomes make peptide bonds by Rodnina, Marina V., Beringer, Malte, Wintermeyer, Wolfgang

“…Ribosomes are molecular machines that synthesize proteins in the cell. Recent biochemical analyses and high-resolution crystal structures of the bacterial…”
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Complete kinetic mechanism of elongation factor Tu-dependent binding of aminoacyl-tRNA to the A site of the E. coli ribosome by Pape, T, Wintermeyer, W, Rodnina, M V

“…The kinetic mechanism of elongation factor Tu (EF-Tu)-dependent binding of Phe-tRNAPhe to the A site of poly(U)-programmed Escherichia coli ribosomes has been…”
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Induced fit in initial selection and proofreading of aminoacyl-tRNA on the ribosome by Pape, Tillmann, Wintermeyer, Wolfgang, Rodnina, Marina

“…The fidelity of aminoacyl‐tRNA (aa‐tRNA) selection by the bacterial ribosome is determined by initial selection before and proofreading after GTP hydrolysis by…”
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Sequence of Steps in Ribosome Recycling as Defined by Kinetic Analysis by Peske, Frank, Rodnina, Marina V., Wintermeyer, Wolfgang

“…After termination of protein synthesis in bacteria, ribosomes are recycled from posttermination complexes by the combined action of elongation factor G (EF-G),…”
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Elongation factor Tu, a GTPase triggered by codon recognition on the ribosome: mechanism and GTP consumption by Rodnina, M V, Pape, T, Fricke, R, Wintermeyer, W

“…The mechanism of elongation factor Tu (EF-Tu) catalyzed aminoacyl-tRNA (aa-tRNA) binding to the A site of the ribosome was studied. Two types of complexes of…”
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