Antigen quantification as in vitro alternative for potency testing of inactivated viral poultry vaccines

Routine batch control of licensed inactivated viral vaccines for poultry usually includes a potency assay as a measure of vaccine efficacy. Potency assays often consist of vaccination-challenge experiments in the target species or in laboratory animals. Instead of measuring the protection of vaccina...

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Bibliographic Details
Published in:	The Veterinary quarterly Vol. 22; no. 4; pp. 223 - 227
Main Authors:	Maas, R.A, Winter, M.P.M. de, Venema, S, Oei, H.L, Claassen, I.J.T.M
Format:	Journal Article
Language:	English
Published:	England Taylor & Francis Group 01-10-2000
Subjects:	Animals ANTIGENE ANTIGENOS ANTIGENS Antigens, Viral - analysis AVES DE CORRAL Dose-Response Relationship, Immunologic ELISA Enzyme-Linked Immunosorbent Assay - methods Enzyme-Linked Immunosorbent Assay - veterinary FOWLS HAEMAGGLUTINATION HN Protein - analysis HN Protein - immunology ID Lelystad, Institute for Animal Science and Health ID-Lelystad, Instituut voor Dierhouderij en Diergezondheid IMMUNISATION IMMUNIZATION IN VITRO INFECTIOUS BRONCHITIS VIRUS Infectious bronchitis virus - immunology INFECTIOUS BURSAL DISEASE VIRUS Infectious bursal disease virus - immunology INMUNIZACION MICROBIAL PROTEINS NEWCASTLE DISEASE VIRUS Newcastle disease virus - enzymology Newcastle disease virus - immunology POTENCY POULTRY Poultry Diseases - immunology Poultry Diseases - prevention & control Poultry Diseases - virology PROTEINAS MICROBIANAS PROTEINE MICROBIENNE Sensitivity and Specificity SEROCONVERSION SIALIDASE Specific Pathogen-Free Organisms TEST ELISA Time Factors VACCIN VACCINATION VACCINES Vaccines, Inactivated - immunology Vaccines, Inactivated - standards VACUNA VACUNACION Viral Fusion Proteins - analysis Viral Fusion Proteins - immunology Viral Vaccines - immunology Viral Vaccines - standards VIRUS BURSITE INFECTIEUSE VIRUS BURSITIS INFECCIOSA VIRUS DE LA ENFERMEDAD DE NEWCASTLE VIRUS MALADIE DE NEWCASTLE VOLAILLE
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Summary:	Routine batch control of licensed inactivated viral vaccines for poultry usually includes a potency assay as a measure of vaccine efficacy. Potency assays often consist of vaccination-challenge experiments in the target species or in laboratory animals. Instead of measuring the protection of vaccinated animals against virulent pathogens, the serological response after vaccination can be quantified for some vaccines. In vitro antigen quantification assays would be attractive alternatives for the current potency assays because the time and costs involved could be greatly reduced and animal use could be avoided. Such in vitro assays will only be acceptable when the correlation between results and efficacy or potency has been demonstrated convincingly. The results of our studies on antigen quantification assays indicate that, in principle, quantification of viral antigens from inactivated oil-adjuvanted vaccines is feasible and reproducible using specially developed antigen capture ELISAs in combination with specific software for statistical analysis of the ELISA data. We have developed methods to quantify the haemagglutination-neuraminidase (HN) and fusion (F) proteins of Newcastle disease virus (NDV), the viral protein 3 (VP3) of the infectious bursal disease virus (IBDV), and the spike-1 (S1) protein of the infectious bronchitis virus (IBV). Vaccination experiments with inactivated ND vaccines indicate that the in vitro quantified HN- or F-proteins of NDV are reliable indicators of the serological response after vaccination.
Bibliography:	L73 2001002935 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0165-2176 1875-5941
DOI:	10.1080/01652176.2000.9695063