Diacerein reduces joint damage, pain behavior and inhibits transient receptor potential vanilloid 1, matrix metalloproteinase and glial cells in rat spinal cord

Aim To investigate the antinociceptive, antiedematogenic and chondroprotective effects of diacerein (DIA) in a model of joint inflammation induced by complete Freund's adjuvant (CFA), as well as to investigate the involvement of metalloproteinase (MMP)‐9, transient receptor potential vanilloid...

Full description

Saved in:

Bibliographic Details
Published in:	International journal of rheumatic diseases Vol. 20; no. 10; pp. 1337 - 1349
Main Authors:	Silva, Morgana Duarte, Cidral‐Filho, Francisco José, Winkelmann‐Duarte, Elisa Cristina, Cargnin‐Ferreira, Eduardo, Calixto, João B., Dutra, Rafael C., Santos, Adair Roberto Soares
Format:	Journal Article
Language:	English
Published:	England Wiley Subscription Services, Inc 01-10-2017
Subjects:	Analgesics - pharmacology Animals Anthraquinones - pharmacology Antirheumatic Agents - pharmacology Arthritis, Experimental - enzymology Arthritis, Experimental - pathology Arthritis, Experimental - prevention & control Arthritis, Experimental - psychology Behavior, Animal - drug effects Capsaicin receptors Cartilage diacerein Edema Edema - enzymology Edema - pathology Edema - prevention & control Freund's Adjuvant Gelatinase B Glial cells Glial fibrillary acidic protein Horns Hypersensitivity joint inflammation Joints - drug effects Joints - pathology Knee Male Matrix metalloproteinase Matrix Metalloproteinase 9 - metabolism Matrix Metalloproteinase Inhibitors - pharmacology Metalloproteinase MMP‐9 Neuroglia - drug effects Neuroglia - enzymology Neuroglia - pathology Nociceptive Pain - chemically induced Nociceptive Pain - enzymology Nociceptive Pain - pathology Nociceptive Pain - psychology Optical density Pain Pain perception Rats, Wistar Rodents Spinal cord Spinal Cord - drug effects Spinal Cord - enzymology Spinal Cord - pathology Spinal Cord - physiopathology Synovial membrane Thermosensing - drug effects Time Factors Transient receptor potential proteins TRPV Cation Channels - antagonists & inhibitors TRPV Cation Channels - metabolism TRPV1 Vocalization, Animal - drug effects diacerein glial cells joint inflammation pain TRPV1 MMP-9
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Aim To investigate the antinociceptive, antiedematogenic and chondroprotective effects of diacerein (DIA) in a model of joint inflammation induced by complete Freund's adjuvant (CFA), as well as to investigate the involvement of metalloproteinase (MMP)‐9, transient receptor potential vanilloid 1 (TRPV1) and glial cells in DIA′s action mechanism. Methods Complete Freund's adjuvant was injected into the knee joint of male rats. We observed mechanical and cold hypersensitivity, vocalization and spontaneous pain‐related behaviors, as well as edema of the knee. Tissue samples of the knee were stained with Cason`s technique and the thickness of the condilus cartilage was measured. Immunohistochemical analysis was performed on the spinal cord using anti‐GFAP (glial fibrillary acidic protein), anti‐MMP and anti‐TRPV1 antibodies. Sections of the dorsal horns of the spinal cord were captured and an optical density was obtained. Results Complete Freund's adjuvant induced mechanical and thermal hypersensitivity, as well as joint edema and changes in the synovial membrane and cartilage. DIA (30 mg/kg, orally, daily) significantly inhibited mechanical (58 ± 10–87 ± 3%) and thermal (66 ± 12–87 ± 8%) hypersensitivity, vocalization (83 ± 5–41 ± 11%), spontaneous pain score, joint swelling (60 ± 6–40 ± 9%), as well as the histological changes induced by CFA. In addition, DIA inhibited astrocyte activation, and prevented the increase of MMP‐9 and TRPV1 expression in the spinal cord of the animals subjected to CFA injections. Conclusions In short, this study shows that DIA reduces joint damage and hypersensitivity associated with inflammation induced by CFA through the inhibition of astroglial activation and decreases the expression of TRPV1 and MMP‐9 in the rat spinal cord.
ISSN:	1756-1841 1756-185X
DOI:	10.1111/1756-185X.12741