Search Results - "Wiltschi, Birgit"

Aligning fermentation conditions with non-canonical amino acid addition strategy is essential for Nε-((2-azidoethoxy)carbonyl)-L-lysine uptake and incorporation into the target protein by Hanaee-Ahvaz, Hana, Baumann, Marina Alexandra, Tauer, Christopher, Albrecht, Bernd, Wiltschi, Birgit, Cserjan-Puschmann, Monika, Striedner, Gerald

“…Protein engineering with non-canonical amino acids (ncAAs) holds great promises for diverse applications, however, there are still limitations in the…”
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Fluoro amino acids: A rarity in nature, yet a prospect for protein engineering by Odar, Corinna, Winkler, Margit, Wiltschi, Birgit

“…Fluoro amino acids are highly valuable compounds constantly gaining relevance in diverse fields of the biosciences as well as in the pharmaceutical industry…”
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Engineering cascade biocatalysis in whole cells for bottom-up synthesis of cello-oligosaccharides: flux control over three enzymatic steps enables soluble production by Schwaiger, Katharina N, Voit, Alena, Wiltschi, Birgit, Nidetzky, Bernd

“…Soluble cello-oligosaccharides (COS, β-1,4-D-gluco-oligosaccharides with degree of polymerization DP 2-6) have been receiving increased attention in different…”
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Performance analysis of orthogonal pairs designed for an expanded eukaryotic genetic code by Nehring, Sebastian, Budisa, Nediljko, Wiltschi, Birgit

“…The suppression of amber stop codons with non-canonical amino acids (ncAAs) is used for the site-specific introduction of many unusual functions into proteins…”
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A bispecific, crosslinking lectibody activates cytotoxic T cells and induces cancer cell death by Rosato, Francesca, Pasupuleti, Rajeev, Tomisch, Jana, Meléndez, Ana Valeria, Kolanovic, Dajana, Makshakova, Olga N, Wiltschi, Birgit, Römer, Winfried

“…Aberrant glycosylation patterns play a crucial role in the development of cancer cells as they promote tumor growth and aggressiveness. Lectins recognize…”
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Substituting the catalytic proline of 4-oxalocrotonate tautomerase with non-canonical analogues reveals a finely tuned catalytic system by Lukesch, Michael S., Pavkov-Keller, Tea, Gruber, Karl, Zangger, Klaus, Wiltschi, Birgit

“…The enzyme 4-oxalocrotonate tautomerase shows remarkable catalytic versatility due to the secondary amine of its N-terminal proline moiety. In this work, we…”
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Synthetic biology of proteins: tuning GFPs folding and stability with fluoroproline by Steiner, Thomas, Hess, Petra, Bae, Jae Hyun, Wiltschi, Birgit, Moroder, Luis, Budisa, Nediljko

“…Proline residues affect protein folding and stability via cis/trans isomerization of peptide bonds and by the C(gamma)-exo or -endo puckering of their…”
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Inhibition of the Fungal Fatty Acid Synthase Type I Multienzyme Complex by Johansson, Patrik, Wiltschi, Birgit, Kumari, Preeti, Kessler, Brigitte, Vonrhein, Clemens, Vonck, Janet, Oesterhelt, Dieter, Grininger, Martin

“…Fatty acids are among the major building blocks of living cells, making lipid biosynthesis a potent target for compounds with antibiotic or antineoplastic…”
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Antibody fragments functionalized with non-canonical amino acids preserving structure and functionality - A door opener for new biological and therapeutic applications by Hanaee-Ahvaz, Hana, Cserjan-Puschmann, Monika, Mayer, Florian, Tauer, Christopher, Albrecht, Bernd, Furtmüller, Paul G., Wiltschi, Birgit, Hahn, Rainer, Striedner, Gerald

“…Functionalization of proteins by incorporating reactive non-canonical amino acids (ncAAs) has been widely applied for numerous biological and therapeutic…”
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A Machine Learning Approach for Efficient Selection of Enzyme Concentrations and Its Application for Flux Optimization by Anamya Ajjolli Nagaraja, Philippe Charton, Xavier F. Cadet, Nicolas Fontaine, Mathieu Delsaut, Birgit Wiltschi, Alena Voit, Bernard Offmann, Cedric Damour, Brigitte Grondin-Perez, Frederic Cadet

“…The metabolic engineering of pathways has been used extensively to produce molecules of interest on an industrial scale. Methods like gene regulation or…”
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Natural history and experimental evolution of the genetic code by Wiltschi, Birgit, Budisa, Nediljko

“…The standard genetic code is a set of rules that relates the 20 canonical amino acids in proteins to groups of three bases in the mRNA. It evolved from a more…”
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Residue-Specific Incorporation of the Non-Canonical Amino Acid Norleucine Improves Lipase Activity on Synthetic Polyesters by Haernvall, Karolina, Fladischer, Patrik, Schoeffmann, Heidemarie, Zitzenbacher, Sabine, Pavkov-Keller, Tea, Gruber, Karl, Schick, Michael, Yamamoto, Motonori, Kuenkel, Andreas, Ribitsch, Doris, Guebitz, Georg M, Wiltschi, Birgit

“…Environmentally friendly functionalization and recycling processes for synthetic polymers have recently gained momentum, and enzymes play a central role in…”
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One, two, many: Strategies to alter the number of carbohydrate binding sites of lectins by Tobola, Felix, Wiltschi, Birgit

“…Carbohydrates are more than an energy-storage. They are ubiquitously found on cells and most proteins, where they encode biological information. Lectins bind…”
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Incorporation of non-canonical amino acids into proteins in yeast by Wiltschi, Birgit

“…•More than 30 non-canonical amino acids have been added to the genetic code of yeasts.•Photocrosslinker amino acids are applied in protein–protein interaction…”
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Evaluation of bicinchoninic acid as a ligand for copper(I)-catalyzed azide-alkyne bioconjugations by Christen, Erik H, Gübeli, Raphael J, Kaufmann, Beate, Merkel, Lars, Schoenmakers, Ronald, Budisa, Nediljko, Fussenegger, Martin, Weber, Wilfried, Wiltschi, Birgit

“…The Cu(I)-catalyzed cycloaddition of terminal azides and alkynes (click chemistry) represents a highly specific reaction for the functionalization of…”
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Biocatalytic Friedel–Crafts Acylation and Fries Reaction by Schmidt, Nina G., Pavkov‐Keller, Tea, Richter, Nina, Wiltschi, Birgit, Gruber, Karl, Kroutil, Wolfgang

“…The Friedel–Crafts acylation is commonly used for the synthesis of aryl ketones, and a biocatalytic version, which may benefit from the chemo‐ and…”
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Fast Protein Modification in the Nanomolar Concentration Range Using an Oxalyl Amide as Latent Thioester by Snella, Benoît, Grain, Benjamin, Vicogne, Jérôme, Capet, Frédéric, Wiltschi, Birgit, Melnyk, Oleg, Agouridas, Vangelis

“…We show that latent oxalyl thioester surrogates are a powerful means to modify peptides and proteins in highly dilute conditions in purified aqueous media or…”
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Enzymes revolutionize the bioproduction of value-added compounds: From enzyme discovery to special applications by Wiltschi, Birgit, Cernava, Tomislav, Dennig, Alexander, Galindo Casas, Meritxell, Geier, Martina, Gruber, Steffen, Haberbauer, Marianne, Heidinger, Petra, Herrero Acero, Enrique, Kratzer, Regina, Luley-Goedl, Christiane, Müller, Christina A., Pitzer, Julia, Ribitsch, Doris, Sauer, Michael, Schmölzer, Katharina, Schnitzhofer, Wolfgang, Sensen, Christoph W., Soh, Jung, Steiner, Kerstin, Winkler, Christoph K., Winkler, Margit, Wriessnegger, Tamara

“…Competitive sustainable production in industry demands new and better biocatalysts, optimized bioprocesses and cost-effective product recovery. Our review…”
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Genetic code expansion in E. coli enables production of a functional ‘ready-to-click’ T cell receptor-specific scFv by Pasupuleti, Rajeev, Rosato, Francesca, Kolanovic, Dajana, Makshakova, Olga N., Römer, Winfried, Wiltschi, Birgit

“…Antibody-based cancer therapies have been evolving at a rapid pace in the pharmaceutical market. Bispecific antibody-drug conjugates that engage immune cells…”
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Protein Engineering mit nicht-kanonischen Aminosäuren by Hotz, Natascha, Marzluf, Tanja, Wiltschi, Birgit

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