A preliminary study of the short circuit current (Isc) responses of sweat gland cells from normal and anhidrotic horses to purinergic and adrenergic agonists

The causal factors of equine anhidrosis have not yet been elucidated but defective electrolyte transport mechanisms in the gland are likely to be involved. To investigate this possibility, experiments were performed on cultured equine sweat gland epithelia from five free‐sweating UK horses (3 intact...

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Bibliographic Details
Published in:	Veterinary dermatology Vol. 18; no. 3; pp. 152 - 160
Main Authors:	Wilson, Darius C. S., Corbett, Alistair D., Steel, Cate, Pannirselvam, Roshni, Bovell, Douglas L.
Format:	Journal Article
Language:	English
Published:	Oxford, UK Blackwell Publishing Ltd 01-06-2007
Subjects:	Adrenergic Agonists - pharmacology Animals Cells, Cultured Dose-Response Relationship, Drug Electrophysiology Epithelial Cells - metabolism Extracellular Space - metabolism Female Horse Diseases - physiopathology Horses - physiology Hypohidrosis - physiopathology Hypohidrosis - veterinary Ion Channels - drug effects Ion Channels - metabolism Ion Transport - drug effects Isoproterenol - pharmacology Male Purines - pharmacology Receptors, Purinergic - metabolism Sweat Glands - cytology Sweat Glands - drug effects Sweating - drug effects
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Summary:	The causal factors of equine anhidrosis have not yet been elucidated but defective electrolyte transport mechanisms in the gland are likely to be involved. To investigate this possibility, experiments were performed on cultured equine sweat gland epithelia from five free‐sweating UK horses (3 intact males, 2 mares, aged 2–4 years) and from three free‐sweating Singapore horses (1 intact male, 2 mares, aged 3–5 years) and three anhidrotic (Singapore) horses (1 intact male, 1 gelding, 1 mare, aged 3–6 years). Cultured cells from each animal were grown on permeable supports and loaded into Ussing chambers to quantify transepithelial resistance and agonist‐induced electrolyte transport by the short circuit current (Isc) technique. Transepithelial resistances across the layers of cultured cells were not significantly different between cells from UK and Singapore free‐sweating horses, but were significantly reduced in anhidrotic animals. Purinergic agonists added to the apical and basolateral aspects of the cultured cells caused similar increases in Isc between the two populations of unaffected cells, but Isc increases were significantly reduced in anhidrotic animals. Beta‐adrenergic agonist stimulation of the anhidrotic cell layers failed to elicit any change in Isc. These pilot results not only confirm earlier conclusions from anatomical findings that failure in the secretory process occurs in anhidrosis but also indicate that both of the known ion transport mechanisms are involved. The trigger for these failures warrants further investigation.
Bibliography:	istex:788E944CBADA629E64C1BD29F8340D0636ABBF55 ark:/67375/WNG-SM7ZVKGH-F ArticleID:VDE585 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0959-4493 1365-3164
DOI:	10.1111/j.1365-3164.2007.00585.x