Search Results - "Wilms, Christian D."
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Calcium Rubies: A Family of Red-Emitting Functionalizable Indicators Suitable for Two-Photon Ca2+ Imaging
Published in Journal of the American Chemical Society (12-09-2012)“…We designed Calcium Rubies, a family of functionalizable BAPTA-based red-fluorescent calcium (Ca2+) indicators as new tools for biological Ca2+ imaging. The…”
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CaRuby-Nano: a novel high affinity calcium probe for dual color imaging
Published in eLife (31-03-2015)“…The great demand for long-wavelength and high signal-to-noise Ca(2+) indicators has led us to develop CaRuby-Nano, a new functionalizable red calcium indicator…”
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Reading out a spatiotemporal population code by imaging neighbouring parallel fibre axons in vivo
Published in Nature communications (09-03-2015)“…The spatiotemporal pattern of synaptic inputs to the dendritic tree is crucial for synaptic integration and plasticity. However, it is not known if input…”
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Photo‐physical properties of Ca2+‐indicator dyes suitable for two‐photon fluorescence‐lifetime recordings
Published in Journal of microscopy (Oxford) (01-03-2007)“…Summary We analyzed the suitability of various Ca2+‐indicator dyes for quantitative two‐photon fluorescence‐lifetime imaging. Although fura‐2, fluo‐3, BTC and…”
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Quantitative two-photon Ca2+ imaging via fluorescence lifetime analysis
Published in Cell calcium (Edinburgh) (01-07-2006)“…Two-photon microscopy (TPM) revolutionized Ca2+ imaging by allowing recordings in the depth of intact tissue and live organisms. A serious limitation in TPM,…”
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Twitching towards the ideal calcium sensor
Published in Nature methods (01-02-2014)“…A new family of genetically encoded ratiometric calcium indicators optimized for imaging of calcium signals in vivo exhibits near-linear fluorescence dynamics…”
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QUAREP‐LiMi: A community‐driven initiative to establish guidelines for quality assessment and reproducibility for instruments and images in light microscopy
Published in Journal of microscopy (Oxford) (01-10-2021)“…A modern day light microscope has evolved from a tool devoted to making primarily empirical observations to what is now a sophisticated , quantitative device…”
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Editorial: Determinants of Synaptic Information Transfer: From Ca2+ Binding Proteins to Ca2+ Signaling Domains
Published in Frontiers in cellular neuroscience (16-03-2016)“…The Editorial on the Research Topic Determinants of Synaptic Information Transfer: From Ca2+ Binding Proteins to Ca2+ Signaling Domains Ca2+ ions are key…”
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Lighting up neural networks using a new generation of genetically encoded calcium sensors
Published in Nature methods (01-12-2009)“…Two improved genetically encoded calcium indicators—based on structure-guided sensor design or on precise subcellular targeting to presynaptic boutons—allow…”
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SpRET: highly sensitive and reliable spectral measurement of absolute FRET efficiency
Published in Microscopy and microanalysis (01-04-2011)“…Contemporary research aims to understand biological processes not only by identifying participating proteins, but also by characterizing the dynamics of their…”
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Twitching towards the ideal calcium sensor: a new family of genetically encoded ratiometric calcium indicators optimized for imaging of calcium signals in vivo exhibits near-linear fluorescence dynamics while minimizing artifacts caused by movement
Published in Nature methods (01-02-2014)Get full text
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Determinants of synaptic information transfer: From Ca2+ binding proteins to Ca2+ signaling domains
Published in Frontiers in cellular neuroscience (01-03-2016)Get full text
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Photo-physical properties of Ca super(2+)-indicator dyes suitable for two-photon fluorescence-lifetime recordings
Published in Journal of microscopy (Oxford) (01-03-2007)“…We analyzed the suitability of various Ca super(2+)-indicator dyes for quantitative two-photon fluorescence-lifetime imaging. Although fura-2, fluo-3, BTC and…”
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Photo‐physical properties of Ca 2+ ‐indicator dyes suitable for two‐photon fluorescence‐lifetime recordings
Published in Journal of microscopy (Oxford) (01-03-2007)“…Summary We analyzed the suitability of various Ca 2+ ‐indicator dyes for quantitative two‐photon fluorescence‐lifetime imaging. Although fura‐2, fluo‐3, BTC…”
Get full text
Journal Article -
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Calcium Rubies: A Family of Red-Emitting Functionalizable Indicators Suitable for Two-Photon Ca 2+ Imaging
Published in Journal of the American Chemical Society (12-09-2012)Get full text
Journal Article -
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Calcium Rubies: a family of red-emitting functionalizable indicators for two-photon Ca2+ imaging
Published in Journal of the American Chemical Society (20-07-2012)“…Calcium Rubies, a family of functionalizable BAPTA-based red-fluorescent calcium (Ca2+) indicators, were designed and synthesized as new tools for…”
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18
Quantitative two-photon Ca super(2+) imaging via fluorescence lifetime analysis
Published in Cell calcium (Edinburgh) (01-07-2006)“…Two-photon microscopy (TPM) revolutionized Ca super(2+) imaging by allowing recordings in the depth of intact tissue and live organisms. A serious limitation…”
Get full text
Journal Article -
19
Quantitative two-photon Ca 2+ imaging via fluorescence lifetime analysis
Published in Cell calcium (Edinburgh) (2006)“…Two-photon microscopy (TPM) revolutionized Ca 2+ imaging by allowing recordings in the depth of intact tissue and live organisms. A serious limitation in TPM,…”
Get full text
Journal Article -
20
QUAREP-LiMi: A community-driven initiative to establish guidelines for quality assessment and reproducibility for instruments and images in light microscopy
Published 27-01-2021“…J. Microsc. 2021;1-18 In April 2020, the QUality Assessment and REProducibility for Instruments and Images in Light Microscopy (QUAREP-LiMi) initiative was…”
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