Serum Metabolic Profiling in Inflammatory Bowel Disease

Serum Metabolic Profiling in Inflammatory Bowel Disease

Background The inflammatory bowel diseases (IBD), Crohn’s disease (CD), and ulcerative colitis (UC), are chronic inflammatory conditions of the gastrointestinal tract whose pathogenesis is not completely understood. 1 H nuclear magnetic resonance (NMR) spectroscopy of serum generates comprehensive m...

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Published in:Digestive diseases and sciences Vol. 57; no. 8; pp. 2157 - 2165
Main Authors: Williams, Horace R. T., Willsmore, James D., Cox, I. Jane, Walker, David G., Cobbold, Jeremy F. L., Taylor-Robinson, Simon D., Orchard, Timothy R.
Format: Journal Article
Language:English
Published: Boston Springer US 01-08-2012
Springer
Springer Nature B.V
Subjects:
Adolescent
Adult
Aged
Analysis
Biochemistry
Blood cholesterol
Case-Control Studies
Colitis, Ulcerative - blood
Crohn Disease - blood
Female
Gastroenterology
Gastrointestinal system
Hepatology
Humans
Inflammatory bowel disease
Male
Medical research
Medicine
Medicine & Public Health
Medicine, Experimental
Metabolites
Metabolome
Middle Aged
Nuclear magnetic resonance spectroscopy
Oncology
Original Article
Physiological aspects
Transplant Surgery
Ulcerative colitis
Young Adult
Metabolic profiling
Inflammatory bowel disease
Metabolomics
Metabonomics
Crohn’s disease
Ulcerative colitis
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Summary:Background The inflammatory bowel diseases (IBD), Crohn’s disease (CD), and ulcerative colitis (UC), are chronic inflammatory conditions of the gastrointestinal tract whose pathogenesis is not completely understood. 1 H nuclear magnetic resonance (NMR) spectroscopy of serum generates comprehensive metabolic profiles, reflecting systemic metabolism, which may be altered in disease states. Aim The aim of this study was to use 1 H NMR-based serum metabolic profiling in the investigation of CD patients, UC patients, and controls, potentially to provide insights into disordered metabolism in IBD, and into underlying mechanisms of disease. Methods Serum metabolic profiles were acquired from 67 individuals (24 CD patients, 20 UC patients, and 23 healthy controls). The multivariate pattern-recognition techniques of principal components analysis (PCA) and partial least squares discriminant analysis with orthogonal signal correction (OSC-PLS-DA) were used to investigate differences between cohorts. Results OSC-PLS-DA distinguished CD and UC cohorts with significant predictive accuracy, highlighting differences in lipid and choline metabolism. Metabolic profiles of both CD and UC cohorts, and the combined IBD cohort, differed significantly from controls: metabolites of importance in the OSC-PLS-DA models included lipoproteins (especially HDL cholesterol), choline, N -acetylglycoprotein, and amino acids. Conclusions 1 H NMR-based metabolic profiling has identified distinct differences in serum metabolic phenotype between CD and UC patients, as well as between IBD patients and controls.
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ISSN:0163-2116
1573-2568
DOI:10.1007/s10620-012-2127-2